(Circular) (Six-base) MAPSORT of: pFN21AE4325.seq Check: 36 from: 1 to: 5689 pFN21AE4325 5689 bp With 182 enzymes: SgfI * March 28, 2012 14:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 2945 3816 4189 1563 Size: 1382 871 373 3063 Fragments arranged by size: 3063 1382 871 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2897 3752 5136 5404 1632 Size: 764 501 855 1384 268 1917 Fragments arranged by size: 1917 1384 855 764 501 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 2986 4520 2986 Size: 1534 4155 AhdI GACnn_n'nnGTC Cuts at: 2972 4418 2972 Size: 1446 4243 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2161 1184 Size: 977 4712 AlwNI CAG_nnn'CTG Cuts at: 2245 2374 3432 5004 2245 Size: 129 1058 1572 2930 Fragments arranged by size: 2930 1572 1058 129 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2683 2998 3224 3376 5485 1493 Size: 1190 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 2141 4243 160 Size: 872 1109 2102 1606 Fragments arranged by size: 2102 1606 1109 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2110 3014 1870 Size: 73 130 37 904 4545 Fragments arranged by size: 4545 904 130 73 37 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2210 3010 4465 618 Size: 325 217 627 423 800 1455 1842 Fragments arranged by size: 1842 1455 800 627 423 325 217 BanII G_rGCy'C Cuts at: 1391 2862 3008 1391 Size: 1471 146 4072 Fragments arranged by size: 4072 1471 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2418 5684 2418 Size: 3266 2423 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2213 2524 3750 4906 1790 Size: 423 311 1226 1156 2573 Fragments arranged by size: 2573 1226 1156 423 311 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2510 4349 1239 Size: 1271 1839 2579 Fragments arranged by size: 2579 1839 1271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2577 3814 4679 4977 2577 Size: 1237 865 298 3289 Fragments arranged by size: 3289 1237 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 2417 5683 2417 Size: 3266 2423 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2093 2986 4122 4520 4794 4941 5352 2093 Size: 893 1136 398 274 147 411 2430 Fragments arranged by size: 2430 1136 893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 1178 Size: 30 165 30 1311 30 4123 Fragments arranged by size: 4123 1311 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 1186 Size: 887 4802 BseYI C'CCAG_C Cuts at: 2870 4892 5388 2870 Size: 2022 496 3171 Fragments arranged by size: 3171 2022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2524 2862 3008 3750 3835 4906 Size: 399 423 311 338 146 742 85 1071 Cuts at: 4906 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 423 399 338 311 146 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2986 4333 4520 1700 Size: 110 1176 1347 187 2869 Fragments arranged by size: 2869 1347 1176 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 2701 1184 Size: 1517 4172 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2649 2011 Size: 638 5051 BstYI r'GATC_y Cuts at: 1972 2328 2412 2418 2514 3019 3386 3771 3788 Size: 356 84 6 96 505 367 385 17 Cuts at: 3788 5240 5326 5338 5684 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 505 385 367 356 346 96 86 84 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 3205 3999 4019 3205 Size: 794 20 4875 Fragments arranged by size: 4875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2505 2993 2505 Size: 488 5201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654 Size: 54 1425 50 275 18 1306 889 1629 Cuts at: 5654 8 Size: 43 Fragments arranged by size: 1629 1425 1306 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2510 2897 3752 4349 5136 5404 Size: 393 764 114 387 855 597 787 268 Cuts at: 5404 1239 Size: 1524 Fragments arranged by size: 1524 855 787 764 597 393 387 268 114 EcoNI CCTnn'n_nnAGG Cuts at: 2180 2180 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2023 2023 Size: 5689 EcoRI G'AATT_C Cuts at: 2683 2998 3376 2683 Size: 315 378 4996 Fragments arranged by size: 4996 378 315 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2635 2635 Size: 5689 FspI TGC'GCA Cuts at: 2034 4195 2034 Size: 2161 3528 FspAI rTGC'GCAy Cuts at: 2034 2034 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2958 2990 4330 4362 4404 Size: 32 135 32 1386 32 1340 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1386 1340 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3647 4006 4165 518 Size: 648 843 1638 359 159 2042 Fragments arranged by size: 2042 1638 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2318 2741 3105 3782 4930 5175 1606 Size: 712 423 364 677 1148 245 2120 Fragments arranged by size: 2120 1148 712 677 423 364 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PfoI T'CCnGG_A Cuts at: 2678 2678 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2156 2234 1613 Size: 543 78 5068 Fragments arranged by size: 5068 543 78 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2609 2641 2609 Size: 32 5657 PstI C_TGCA'G Cuts at: 830 2184 3041 830 Size: 1354 857 3478 Fragments arranged by size: 3478 1354 857 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2318 2318 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2606 3937 5649 2606 Size: 1331 1712 2646 Fragments arranged by size: 2646 1712 1331 SexAI A'CCwGG_T Cuts at: 2648 2648 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2180 2579 3037 4172 4853 5044 826 Size: 219 1135 399 458 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 681 458 399 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2112 3016 1872 Size: 240 904 4545 Fragments arranged by size: 4545 904 240 SmlI C'TyrA_G Cuts at: 820 1017 1943 2592 3793 4694 4956 820 Size: 197 926 649 1201 901 262 1553 Fragments arranged by size: 1553 1201 926 901 649 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2918 1517 Size: 1401 4288 StyI C'CwwG_G Cuts at: 513 1065 3128 513 Size: 552 2063 3074 Fragments arranged by size: 3074 2063 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 2791 3716 3733 3886 4071 4516 Size: 1255 557 654 925 17 153 185 445 Cuts at: 4516 325 Size: 1498 Fragments arranged by size: 1498 1255 925 654 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2604 3935 5647 Size: 274 80 33 51 1443 627 1331 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1331 627 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2112 2144 2839 2871 5649 5681 2112 Size: 32 695 32 2778 32 2120 Fragments arranged by size: 2778 2120 695 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2110 3014 1870 Size: 240 904 4545 Fragments arranged by size: 4545 904 240 XmnI GAAnn'nnTTC Cuts at: 2606 3818 2606 Size: 1212 4477 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI