################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BglII BlpI BpmI Bpu1102I BsaHI BsaI BsgI BsiEI BsiWI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MflI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 1134 TCCGGA T^CCGGA AfaI 347 GTAC GT^AC Alw44I 931 GTGCAC G^TGCAC AlwI 1108 GATCC GGATC(4/5) AlwNI 1094 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 815 GGGCCC GGGCC^C ApaLI 931 GTGCAC G^TGCAC AseI 909 ATTAAT AT^TAAT AsnI 909 ATTAAT AT^TAAT AvrII 936 CCTAGG C^CTAGG BalI 584 TGGCCA TGG^CCA BanI 574 GGTGCC G^G[CT][GA]CC BanII 815 GGGCCC G[GA]GC[CT]^C BbsI 96 GAAGAC GAAGAC(2/6) BcnI 350 CCCGG CC^[GC]GG BglI 577 GCCCTCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 936 CCTAGG C^CTAGG BpuAI 96 GAAGAC GAAGAC(2/6) BsaAI 232 TACGTG [CT]AC^GT[GA] BsaBI 354 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1134 TCCGGA [AT]^CCGG[AT] BseAI 1134 TCCGGA T^CCGGA BseRI 868 GAGGAG GAGGAG(10/8) BsmFI 1096 GTCCC GGGAC(10/14) BsmI 791 GAATGC GAATGC(1/-1) BspEI 1134 TCCGGA T^CCGGA BspHI 521 TCATGA T^CATGA BspMI 927 GCAGGT ACCTGC(4/8) BstXI 880 CCATCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 49 GCGC GCG^C DdeI 1266 CTCAG C^T[ACGT]AG DsaI 567 CCATGG C^C[GA][CT]GG EaeI 584 TGGCCA [CT]^GGCC[GA] EcoT22I 244 ATGCAT ATGCA^T FauI 267 GCGGG CCCGC(4/6) HhaI 49 GCGC GCG^C HinP1I 49 GCGC G^CGC HphI 1125 GGTGA GGTGA(8/7) MamI 354 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1195 CAATTG C^AATTG MluNI 584 TGGCCA TGG^CCA MroI 1134 TCCGGA T^CCGGA MscI 584 TGGCCA TGG^CCA MspA1I 1268 CAGCTG C[AC]G^C[GT]G MunI 1195 CAATTG C^AATTG NciI 350 CCCGG CC^[GC]GG NcoI 567 CCATGG C^CATGG NsiI 244 ATGCAT ATGCA^T PleI 1305 GAGTC GAGTC(4/5) PshAI 543 GACGTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 909 ATTAAT AT^TAAT PvuII 1268 CAGCTG CAG^CTG RcaI 521 TCATGA T^CATGA RsaI 347 GTAC GT^AC SmlI 495 CTCAAG C^T[CT][GA]AG TaqI 459 TCGA T^CGA Tsp45I 124 GTCAC ^GT[GC]AC TthHB8I 459 TCGA T^CGA XcmI 983 CCAATGTAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 462 AAATTT [GA]^AATT[CT] 1251 AAATTT ApoI 462 AAATTT [GA]^AATT[CT] 1251 AAATTT AspHI 931 GTGCAC G[AT]GC[AT]^C 1157 GTGCTC BclI 338 TGATCA T^GATCA 960 TGATCA BsiHKAI 931 GTGCAC G[AT]GC[AT]^C 1157 GTGCTC BsmBI 541 GAGACG CGTCTC(1/5) 716 GAGACG BssSI 580 CTCGTG CACGAG(-5/-1) 863 CTCGTG BstF5I 881 CATCC GGATG(2/0) 80 GGATG Cfr13I 815 GGGCC G^G[ACGT]CC 816 GGCCC FbaI 338 TGATCA T^GATCA 960 TGATCA FokI 881 CATCC GGATG(9/13) 80 GGATG HaeIII 585 GGCC GG^CC 816 GGCC HapII 351 CCGG C^CGG 1135 CCGG HindIII 639 AAGCTT A^AGCTT 874 AAGCTT HpaII 351 CCGG C^CGG 1135 CCGG MspI 351 CCGG C^CGG 1135 CCGG NlaIV 574 GGTGCC GG[ACGT]^[ACGT]CC 815 GGGCCC Sau96I 815 GGGCC G^G[ACGT]CC 816 GGCCC SfaNI 472 GATGC GCATC(5/9) 422 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI BbvI BciVI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI DpnII EcoRII EcoT14I Fnu4HI HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI