Restriction map for pFN21AE4426
 (Circular) (Six-base) MAPSORT of: pFN21AE4426.seq  Check: 1221  from: 1  to: 6034



pFN21AE4426  6034 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 13:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2923    2923 
   Size:       6034
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3844     278 
   Size:         53      83     186     936    2308    2468

  Fragments arranged by size: 

                2468    2308     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3377    1937 
   Size:       1440    4594
 
Acc65I G'GTAC_C

Cuts at:   3355    3355 
   Size:       6034
 
AclI AA'CG_TT

Cuts at:   1563    4161    4534    1563 
   Size:       2598     373    3063

  Fragments arranged by size: 

                3063    2598     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3142    4097    5481    5749    1632 
   Size:       1510     955    1384     268    1917

  Fragments arranged by size: 

                1917    1510    1384     955     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5837
 
AflIII A'CryG_T

Cuts at:   1203    4933    5910    1203 
   Size:       3730     977    1327

  Fragments arranged by size: 

                3730    1327     977
 
AgeI A'CCGG_T

Cuts at:   4865    4865 
   Size:       6034
 
AhdI GACnn_n'nnGTC

Cuts at:   4763    4763 
   Size:       6034
 
AleI CACnn'nnGTG

Cuts at:   1166    2943    3128    1166 
   Size:       1777     185    4072

  Fragments arranged by size: 

                4072    1777     185
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6034
 
AlwNI CAG_nnn'CTG

Cuts at:   3104    3777    5349    3104 
   Size:        673    1572    3789

  Fragments arranged by size: 

                3789    1572     673
 
ApaI G_GGCC'C

Cuts at:   2824    2824 
   Size:       6034
 
ApaLI G'TGCA_C

Cuts at:   2936    4091    5247    2936 
   Size:       1155    1156    3723

  Fragments arranged by size: 

                3723    1156    1155
 
ApoI r'AATT_y

Cuts at:   1493    2467    3256    3343    3569    3721    5830    1493 
   Size:        974     789      87     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     974     789     226     152      87
 
AseI AT'TA_AT

Cuts at:    160    1032    2915    4588     160 
   Size:        872    1883    1673    1606

  Fragments arranged by size: 

                1883    1673    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6034
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3359    1870 
   Size:         73    1416    4545

  Fragments arranged by size: 

                4545    1416      73
 
AvrII C'CTAG_G

Cuts at:   2941    2941 
   Size:       6034
 
BamHI G'GATC_C

Cuts at:   3364    3731    3364 
   Size:        367    5667
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2579    3355    4810     618 
   Size:        325     217     627     792     776    1455    1842

  Fragments arranged by size: 

                1842    1455     792     776     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2824    3353    1391 
   Size:       1433     529    4072

  Fragments arranged by size: 

                4072    1433     529
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2108     928 
   Size:        971     209    4854

  Fragments arranged by size: 

                4854     971     209
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4225    4259    1903 
   Size:         34    2288      34    3678

  Fragments arranged by size: 

                3678    2288      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2352    2762    2790    3926    5136    2352 
   Size:        410      28    1136    1210    3250

  Fragments arranged by size: 

                3250    1210    1136     410      28
 
BclI T'GATC_A

Cuts at:   1548    2343    2965    1548 
   Size:        795     622    4617

  Fragments arranged by size: 

                4617     795     622
 
BfrBI ATG'CAT

Cuts at:   2251    2251 
   Size:       6034
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2588    4645     136 
   Size:        107     122      71     631     605     916    2057    1525

  Fragments arranged by size: 

                2057    1525     916     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   6029    6029 
   Size:       6034
 
BlpI GC'TnA_GC

Cuts at:   3451    3451 
   Size:       6034
 
Bme1580I G_kGCm'C

Cuts at:   1790    2584    2824    2940    4095    5251    1790 
   Size:        794     240     116    1155    1156    2573

  Fragments arranged by size: 

                2573    1156    1155     794     240     116
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6034
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2634    2818    3209 
   Size:        416      53      83     399      24    1217     184     391

Cuts at:   3209    3765    4723     442 
   Size:        556     958    1753

  Fragments arranged by size: 

                1753    1217     958     556     416     399     391     184
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6034
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4694    1239 
   Size:       3455    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2485    4159    5024    5322    2485 
   Size:       1674     865     298    3197

  Fragments arranged by size: 

                3197    1674     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4697     882 
   Size:        617    3198    2219

  Fragments arranged by size: 

                3198    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2239    3825     493 
   Size:        711    1035    1586    2702

  Fragments arranged by size: 

                2702    1586    1035     711
 
BsaBI GATnn'nnATC

Cuts at:   2363    6028    2363 
   Size:       3665    2369
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3841    4223    4858     275 
   Size:         53      83     186     936    2308     382     635    1451

  Fragments arranged by size: 

                2308    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3139    4467    4865    5139    5286    5697    3139 
   Size:       1328     398     274     147     411    3476

  Fragments arranged by size: 

                3476    1328     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5809

  Fragments arranged by size: 

                5809     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2888    1186 
   Size:       1702    4332
 
BseYI C'CCAG_C

Cuts at:   5237    5733    5237 
   Size:        496    5538
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5461
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3484    4245    5273    1540 
   Size:        275     186    1483     761    1028    2301

  Fragments arranged by size: 

                2301    1483    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2940    3166    4095    4180    5251    2940 
   Size:        226     929      85    1071    3723

  Fragments arranged by size: 

                3723    1071     929     226      85
 
BsmI GAATG_Cn'

Cuts at:   2802    3545    3638    2802 
   Size:        743      93    5198

  Fragments arranged by size: 

                5198     743      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2540    2715    2540 
   Size:        175    5859
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2584    2824    2940    3166    3353    4095    4180 
   Size:        399     794     240     116     226     187     742      85

Cuts at:   4180    5251    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     794     742     399     240     226     187
                 116      85
 
BspEI T'CCGG_A

Cuts at:   3139    5697    3139 
   Size:       2558    3476
 
BspHI T'CATG_A

Cuts at:   2526    3923    5653    2526 
   Size:       1397    1730    2907

  Fragments arranged by size: 

                2907    1730    1397
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2923    3389     844 
   Size:       2079     466    3489

  Fragments arranged by size: 

                3489    2079     466
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3921    1394 
   Size:        429    2098    3507

  Fragments arranged by size: 

                3507    2098     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4529    4703      65 
   Size:       1183    3281     174    1396

  Fragments arranged by size: 

                3281    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4678    4865    1700 
   Size:        110    2868     187    2869

  Fragments arranged by size: 

                2869    2868     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6034
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6034
 
BssSI C'ACGA_G

Cuts at:   2585    2868    4094    5106    2585 
   Size:        283    1226    1012    3513

  Fragments arranged by size: 

                3513    1226    1012     283
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6034
 
BstBI TT'CG_AA

Cuts at:   4838    4838 
   Size:       6034
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6034
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2892    2892 
   Size:       6034
 
BstYI r'GATC_y

Cuts at:   1972    3364    3731    4116    4133    5585    5671    5683    6029 
   Size:       1392     367     385      17    1452      86      12     346

Cuts at:   6029    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1392     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2572     513 
   Size:        552     939     568    3975

  Fragments arranged by size: 

                3975     939     568     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5295

  Fragments arranged by size: 

                5295     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3550    4344    4364    3550 
   Size:        794      20    5220

  Fragments arranged by size: 

                5220     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6034
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5999
 
DraI TTT'AAA

Cuts at:   3338    3338 
   Size:       6034
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5041     809 
   Size:        507    3725    1802

  Fragments arranged by size: 

                3725    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2589    3481    4370 
   Size:         54    1425      50     275      18     759     892     889

Cuts at:   4370    5999       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     892     889     759     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3481    1537 
   Size:        275    1669    4090

  Fragments arranged by size: 

                4090    1669     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3964    1353 
   Size:       2611    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4621    4995    5141     387 
   Size:       1064     138    3032     374     146    1280

  Fragments arranged by size: 

                3032    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3142    4097    4694    5481    5749    1239 
   Size:        393    1510     955     597     787     268    1524

  Fragments arranged by size: 

                1524    1510     955     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   3343    3721    3343 
   Size:        378    5656
 
FspI TGC'GCA

Cuts at:   4540    4540 
   Size:       6034
 
HaeII r_GCGC'y

Cuts at:   1678    5181    1678 
   Size:       3503    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2284    2316    4675    4707    4749 
   Size:         32     135      32     712      32    2359      32      42

Cuts at:   4749    4781    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2359     712     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3378    3624     669 
   Size:        988     281    1440     246    3079

  Fragments arranged by size: 

                3079    1440     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2644    2879     748 
   Size:       1896     235    3903

  Fragments arranged by size: 

                3903    1896     235
 
HpaI GTT'AAC

Cuts at:   3624    3624 
   Size:       6034
 
KpnI G_GTAC'C

Cuts at:   3359    3359 
   Size:       6034
 
MfeI C'AATT_G

Cuts at:   3200    3633    3200 
   Size:        433    5601
 
MluI A'CGCG_T

Cuts at:   5910    5910 
   Size:       6034
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2823    5148    5332    1305 
   Size:       1518    2325     184    2007

  Fragments arranged by size: 

                2325    2007    1518     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2591      10 
   Size:         54    1425     343     759    3453

  Fragments arranged by size: 

                3453    1425     759     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2256    2577    2688    2943    3128    3992    4351 
   Size:        648    1090     321     111     255     185     864     359

Cuts at:   4351    4510     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1090     864     648     359     321     255     185
                 159     111
 
MspA1I CmG'CkG

Cuts at:   1606    3275    3450    4127    5275    5520    1606 
   Size:       1669     175     677    1148     245    2120

  Fragments arranged by size: 

                2120    1669    1148     677     245     175
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2572     513 
   Size:        552     939     568    3975

  Fragments arranged by size: 

                3975     939     568     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6034
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6034
 
NotI GC'GGCC_GC

Cuts at:   3481    3481 
   Size:       6034
 
NsiI A_TGCA'T

Cuts at:   2253    2253 
   Size:       6034
 
NspI r_CATG'y

Cuts at:   1132    1229    3392    4937    1132 
   Size:         97    2163    1545    2229

  Fragments arranged by size: 

                2229    2163    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6034
 
PciI A'CATG_T

Cuts at:   4933    4933 
   Size:       6034
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6034
 
PmeI GTTT'AAAC

Cuts at:   3338    3338 
   Size:       6034
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4103    4135    5616    5648    4103 
   Size:         32    1481      32    4489

  Fragments arranged by size: 

                4489    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2552    1613 
   Size:        939    5095
 
PsiI TTA'TAA

Cuts at:   2445    3604    2445 
   Size:       1159    4875
 
PspOMI G'GGCC_C

Cuts at:   2820    2820 
   Size:       6034
 
PstI C_TGCA'G

Cuts at:    830    3386     830 
   Size:       2556    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6034
 
PvuII CAG'CTG

Cuts at:   3275    3275 
   Size:       6034
 
SalI G'TCGA_C

Cuts at:   1936    3376    1936 
   Size:       1440    4594
 
SbfI CC_TGCA'GG

Cuts at:   3386    3386 
   Size:       6034
 
ScaI AGT'ACT

Cuts at:   4282    5994    4282 
   Size:       1712    4322
 
SfcI C'TryA_G

Cuts at:    826    1045    2119    2422    3031    3382    4517    5198    5389 
   Size:        219    1074     303     609     351    1135     681     191

Cuts at:   5389     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135    1074     681     609     351     303     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6034
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6034
 
SmaI CCC'GGG

Cuts at:   1872    3361    1872 
   Size:       1489    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2500    4138    5039    5301     820 
   Size:        197     926     557    1638     901     262    1553

  Fragments arranged by size: 

                1638    1553     926     901     557     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3825     493 
   Size:       3332    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6034
 
SphI G_CATG'C

Cuts at:   1132    3392    1132 
   Size:       2260    3774
 
SspI AAT'ATT

Cuts at:      5      52    3958       5 
   Size:         47    3906    2081

  Fragments arranged by size: 

                3906    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6034
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2572    2788    2941    3193    3473     513 
   Size:        552     939     568     216     153     252     280    3074

  Fragments arranged by size: 

                3074     939     568     552     280     252     216     153
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4061    4078    4231    4416    4861     325 
   Size:       1255    2481      17     153     185     445    1498

  Fragments arranged by size: 

                2481    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4280    5992      96 
   Size:        274      80      33      51    1443    2303    1712     138

  Fragments arranged by size: 

                2303    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2000    2032    5994    6026    2000 
   Size:         32    3962      32    2008

  Fragments arranged by size: 

                3962    2008      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6034
 
XbaI T'CTAG_A

Cuts at:   3370    3370 
   Size:       6034
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2995    2995 
   Size:       6034
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6034
 
XmaI C'CCGG_G

Cuts at:   1870    3359    1870 
   Size:       1489    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4163    4163 
   Size:       6034
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3842     276 
   Size:         53      83     186     936    2308    2468

  Fragments arranged by size: 

                2468    2308     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI     BaeI     BbeI    BbvCI     BplI   Bpu10I
    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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