(Circular) (Six-base) MAPSORT of: pFN21AE4426.seq Check: 1221 from: 1 to: 6034 pFN21AE4426 6034 bp With 182 enzymes: SgfI * March 28, 2012 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2923 2923 Size: 6034 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3377 1937 Size: 1440 4594 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3142 4097 5481 5749 1632 Size: 1510 955 1384 268 1917 Fragments arranged by size: 1917 1510 1384 955 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 2943 3128 1166 Size: 1777 185 4072 Fragments arranged by size: 4072 1777 185 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AlwNI CAG_nnn'CTG Cuts at: 3104 3777 5349 3104 Size: 673 1572 3789 Fragments arranged by size: 3789 1572 673 ApaI G_GGCC'C Cuts at: 2824 2824 Size: 6034 ApaLI G'TGCA_C Cuts at: 2936 4091 5247 2936 Size: 1155 1156 3723 Fragments arranged by size: 3723 1156 1155 ApoI r'AATT_y Cuts at: 1493 2467 3256 3343 3569 3721 5830 1493 Size: 974 789 87 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 789 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 2915 4588 160 Size: 872 1883 1673 1606 Fragments arranged by size: 1883 1673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 3359 1870 Size: 73 1416 4545 Fragments arranged by size: 4545 1416 73 AvrII C'CTAG_G Cuts at: 2941 2941 Size: 6034 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3355 4810 618 Size: 325 217 627 792 776 1455 1842 Fragments arranged by size: 1842 1455 792 776 627 325 217 BanII G_rGCy'C Cuts at: 1391 2824 3353 1391 Size: 1433 529 4072 Fragments arranged by size: 4072 1433 529 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2108 928 Size: 971 209 4854 Fragments arranged by size: 4854 971 209 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4225 4259 1903 Size: 34 2288 34 3678 Fragments arranged by size: 3678 2288 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2352 2762 2790 3926 5136 2352 Size: 410 28 1136 1210 3250 Fragments arranged by size: 3250 1210 1136 410 28 BclI T'GATC_A Cuts at: 1548 2343 2965 1548 Size: 795 622 4617 Fragments arranged by size: 4617 795 622 BfrBI ATG'CAT Cuts at: 2251 2251 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4645 136 Size: 107 122 71 631 605 916 2057 1525 Fragments arranged by size: 2057 1525 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2584 2824 2940 4095 5251 1790 Size: 794 240 116 1155 1156 2573 Fragments arranged by size: 2573 1156 1155 794 240 116 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2634 2818 3209 Size: 416 53 83 399 24 1217 184 391 Cuts at: 3209 3765 4723 442 Size: 556 958 1753 Fragments arranged by size: 1753 1217 958 556 416 399 391 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4694 1239 Size: 3455 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2485 4159 5024 5322 2485 Size: 1674 865 298 3197 Fragments arranged by size: 3197 1674 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4697 882 Size: 617 3198 2219 Fragments arranged by size: 3198 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2239 3825 493 Size: 711 1035 1586 2702 Fragments arranged by size: 2702 1586 1035 711 BsaBI GATnn'nnATC Cuts at: 2363 6028 2363 Size: 3665 2369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3139 4467 4865 5139 5286 5697 3139 Size: 1328 398 274 147 411 3476 Fragments arranged by size: 3476 1328 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5809 Fragments arranged by size: 5809 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2888 1186 Size: 1702 4332 BseYI C'CCAG_C Cuts at: 5237 5733 5237 Size: 496 5538 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3484 4245 5273 1540 Size: 275 186 1483 761 1028 2301 Fragments arranged by size: 2301 1483 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2940 3166 4095 4180 5251 2940 Size: 226 929 85 1071 3723 Fragments arranged by size: 3723 1071 929 226 85 BsmI GAATG_Cn' Cuts at: 2802 3545 3638 2802 Size: 743 93 5198 Fragments arranged by size: 5198 743 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2540 2715 2540 Size: 175 5859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2584 2824 2940 3166 3353 4095 4180 Size: 399 794 240 116 226 187 742 85 Cuts at: 4180 5251 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 794 742 399 240 226 187 116 85 BspEI T'CCGG_A Cuts at: 3139 5697 3139 Size: 2558 3476 BspHI T'CATG_A Cuts at: 2526 3923 5653 2526 Size: 1397 1730 2907 Fragments arranged by size: 2907 1730 1397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2923 3389 844 Size: 2079 466 3489 Fragments arranged by size: 3489 2079 466 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4529 4703 65 Size: 1183 3281 174 1396 Fragments arranged by size: 3281 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4678 4865 1700 Size: 110 2868 187 2869 Fragments arranged by size: 2869 2868 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 2585 2868 4094 5106 2585 Size: 283 1226 1012 3513 Fragments arranged by size: 3513 1226 1012 283 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6034 BstXI CCAn_nnnn'nTGG Cuts at: 2892 2892 Size: 6034 BstYI r'GATC_y Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029 Size: 1392 367 385 17 1452 86 12 346 Cuts at: 6029 1972 Size: 1977 Fragments arranged by size: 1977 1452 1392 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2572 513 Size: 552 939 568 3975 Fragments arranged by size: 3975 939 568 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4344 4364 3550 Size: 794 20 5220 Fragments arranged by size: 5220 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 3481 4370 Size: 54 1425 50 275 18 759 892 889 Cuts at: 4370 5999 8 Size: 1629 43 Fragments arranged by size: 1629 1425 892 889 759 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3481 1537 Size: 275 1669 4090 Fragments arranged by size: 4090 1669 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3964 1353 Size: 2611 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4621 4995 5141 387 Size: 1064 138 3032 374 146 1280 Fragments arranged by size: 3032 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3142 4097 4694 5481 5749 1239 Size: 393 1510 955 597 787 268 1524 Fragments arranged by size: 1524 1510 955 787 597 393 268 EcoRI G'AATT_C Cuts at: 3343 3721 3343 Size: 378 5656 FspI TGC'GCA Cuts at: 4540 4540 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 5181 1678 Size: 3503 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 4675 4707 4749 Size: 32 135 32 712 32 2359 32 42 Cuts at: 4749 4781 1373 Size: 32 2626 Fragments arranged by size: 2626 2359 712 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3378 3624 669 Size: 988 281 1440 246 3079 Fragments arranged by size: 3079 1440 988 281 246 HindIII A'AGCT_T Cuts at: 748 2644 2879 748 Size: 1896 235 3903 Fragments arranged by size: 3903 1896 235 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3200 3633 3200 Size: 433 5601 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2823 5148 5332 1305 Size: 1518 2325 184 2007 Fragments arranged by size: 2325 2007 1518 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3453 Fragments arranged by size: 3453 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2256 2577 2688 2943 3128 3992 4351 Size: 648 1090 321 111 255 185 864 359 Cuts at: 4351 4510 518 Size: 159 2042 Fragments arranged by size: 2042 1090 864 648 359 321 255 185 159 111 MspA1I CmG'CkG Cuts at: 1606 3275 3450 4127 5275 5520 1606 Size: 1669 175 677 1148 245 2120 Fragments arranged by size: 2120 1669 1148 677 245 175 NcoI C'CATG_G Cuts at: 513 1065 2004 2572 513 Size: 552 939 568 3975 Fragments arranged by size: 3975 939 568 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NsiI A_TGCA'T Cuts at: 2253 2253 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 3392 4937 1132 Size: 97 2163 1545 2229 Fragments arranged by size: 2229 2163 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6034 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6034 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2552 1613 Size: 939 5095 PsiI TTA'TAA Cuts at: 2445 3604 2445 Size: 1159 4875 PspOMI G'GGCC_C Cuts at: 2820 2820 Size: 6034 PstI C_TGCA'G Cuts at: 830 3386 830 Size: 2556 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 3275 3275 Size: 6034 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 2119 2422 3031 3382 4517 5198 5389 Size: 219 1074 303 609 351 1135 681 191 Cuts at: 5389 826 Size: 1471 Fragments arranged by size: 1471 1135 1074 681 609 351 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3361 1872 Size: 1489 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2500 4138 5039 5301 820 Size: 197 926 557 1638 901 262 1553 Fragments arranged by size: 1638 1553 926 901 557 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 3958 5 Size: 47 3906 2081 Fragments arranged by size: 3906 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2004 2572 2788 2941 3193 3473 513 Size: 552 939 568 216 153 252 280 3074 Fragments arranged by size: 3074 939 568 552 280 252 216 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4061 4078 4231 4416 4861 325 Size: 1255 2481 17 153 185 445 1498 Fragments arranged by size: 2481 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4280 5992 96 Size: 274 80 33 51 1443 2303 1712 138 Fragments arranged by size: 2303 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5994 6026 2000 Size: 32 3962 32 2008 Fragments arranged by size: 3962 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6034 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2995 2995 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 3359 1870 Size: 1489 4545 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI