################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspHI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KspI MaeII MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 54 GGTACC G^GTACC AccI 757 GTCGAC GT^[AC][GT]AC AciI 459 CCGC CCGC(-3/-1) Asp718I 54 GGTACC G^GTACC AspI 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 1135 GTATCC GTATCC(6/5) BfaI 1024 CTAG C^TAG BglI 592 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 266 AGATCT A^GATCT BsaBI 1062 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 169 GAGGAG GAGGAG(10/8) BsmFI 438 GGGAC GGGAC(10/14) BsmI 1289 GAATGC GAATGC(1/-1) BsrDI 587 GCAATG GCAATG(2/0) BsrFI 1250 ACCGGC [GA]^CCGG[CT] BstAPI 1044 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 919 GGTGACC G^GT[ACGT]ACC BstF5I 501 CATCC GGATG(2/0) BstPI 919 GGTGACC G^GT[ACGT]ACC BstYI 266 AGATCT [GA]^GATC[CT] Cfr10I 1250 ACCGGC [GA]^CCGG[CT] DraII 392 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 392 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 919 GGTGACC G^GT[ACGT]ACC EcoT22I 1042 ATGCAT ATGCA^T FauI 458 CCCGC CCCGC(4/6) FokI 501 CATCC GGATG(9/13) KpnI 54 GGTACC GGTAC^C MaeI 1024 CTAG C^TAG MaeIII 920 GTGAC ^GT[ACGT]AC MamI 1062 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 266 AGATCT [GA]^GATC[CT] NsiI 1042 ATGCAT ATGCA^T NspI 311 GCATGT [GA]CATG^[CT] PflFI 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SalI 757 GTCGAC G^TCGAC SmaI 412 CCCGGG CCC^GGG TaqI 758 TCGA T^CGA Tsp45I 920 GTGAC ^GT[GC]AC Tth111I 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 758 TCGA T^CGA XhoII 266 AGATCT [GA]^GATC[CT] XmaCI 412 CCCGGG C^CCGGG XmaI 412 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 391 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1112 CAGCTACTG AseI 670 ATTAAT AT^TAAT 687 ATTAAT AsnI 670 ATTAAT AT^TAAT 687 ATTAAT AvaI 412 CCCGGG C^[CT]CG[GA]G 638 CTCGGG AvaII 900 GGTCC G^G[AT]CC 1248 GGACC BalI 206 TGGCCA TGG^CCA 320 TGGCCA BanII 379 GGGCTC G[GA]GC[CT]^C 415 GGGCTC BsaI 88 GAGACC GGTCTC(1/5) 448 GAGACC BsgI 124 GTGCAG GTGCAG(16/14) 812 GTGCAG BsoBI 412 CCCGGG C^[CT]CG[GA]G 638 CTCGGG BspHI 611 TCATGA T^CATGA 1265 TCATGA EarI 142 GAAGAG CTCTTC(1/4) 187 GAAGAG HincII 726 GTTGAC GT[CT]^[GA]AC 757 GTCGAC HindII 726 GTTGAC GT[CT]^[GA]AC 757 GTCGAC Ksp632I 142 GAAGAG CTCTTC(1/4) 187 GAAGAG MluNI 206 TGGCCA TGG^CCA 320 TGGCCA MscI 206 TGGCCA TGG^CCA 320 TGGCCA PshBI 670 ATTAAT AT^TAAT 687 ATTAAT RcaI 611 TCATGA T^CATGA 1265 TCATGA SfaNI 336 GATGC GCATC(5/9) 1041 GATGC XcmI 887 CCACCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 950 CCACAGACTTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AfaI AluI ApoI BanI BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstXI Cac8I Cfr13I DdeI DpnII EaeI EcoNI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp509I TspRI