(Circular) (Six-base) MAPSORT of: pFN21AE6413.seq Check: 5438 from: 1 to: 6037 pFN21AE6413 6037 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2763 3380 1937 Size: 826 617 4594 Fragments arranged by size: 4594 826 617 Acc65I G'GTAC_C Cuts at: 2059 3358 2059 Size: 1299 4738 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2423 4100 5484 5752 1632 Size: 791 1677 1384 268 1917 Fragments arranged by size: 1917 1677 1384 791 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 4936 5913 1203 Size: 3733 977 1327 Fragments arranged by size: 3733 1327 977 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 2050 1166 Size: 884 5153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2144 2244 1184 Size: 960 100 4977 Fragments arranged by size: 4977 960 100 AlwNI CAG_nnn'CTG Cuts at: 2401 3122 3780 5352 2401 Size: 721 658 1572 3086 Fragments arranged by size: 3086 1572 721 658 ApaLI G'TGCA_C Cuts at: 4094 5250 4094 Size: 1156 4881 ApoI r'AATT_y Cuts at: 1493 2584 2652 2848 3346 3572 3724 5833 1493 Size: 1091 68 196 498 226 152 2109 1697 Fragments arranged by size: 2109 1697 1091 498 226 196 152 68 AseI AT'TA_AT Cuts at: 160 1032 2676 2693 4591 160 Size: 872 1644 17 1898 1606 Fragments arranged by size: 1898 1644 1606 872 17 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2643 3362 1870 Size: 73 474 226 719 4545 Fragments arranged by size: 4545 719 474 226 73 BamHI G'GATC_C Cuts at: 3367 3734 3367 Size: 367 5670 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2059 2800 3358 4813 Size: 325 217 627 227 45 741 558 1455 Cuts at: 4813 618 Size: 1842 Fragments arranged by size: 1842 1455 741 627 558 325 227 217 45 BanII G_rGCy'C Cuts at: 1391 2388 2424 3356 1391 Size: 997 36 932 4072 Fragments arranged by size: 4072 997 932 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2293 2743 2949 928 Size: 971 394 450 206 4016 Fragments arranged by size: 4016 971 450 394 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4228 4262 1903 Size: 34 2291 34 3678 Fragments arranged by size: 3678 2291 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3151 3929 5139 3151 Size: 778 1210 4049 Fragments arranged by size: 4049 1210 778 BclI T'GATC_A Cuts at: 1548 1548 Size: 6037 BfrBI ATG'CAT Cuts at: 3049 3049 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2603 4648 136 Size: 107 122 71 631 605 931 2045 1525 Fragments arranged by size: 2045 1525 931 631 605 122 107 71 BglII A'GATC_T Cuts at: 2271 6032 2271 Size: 3761 2276 BlpI GC'TnA_GC Cuts at: 3454 3454 Size: 6037 Bme1580I G_kGCm'C Cuts at: 1790 2805 3188 4098 5254 1790 Size: 1015 383 910 1156 2573 Fragments arranged by size: 2573 1156 1015 910 383 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2070 2486 3768 Size: 416 53 83 399 24 653 416 1282 Cuts at: 3768 4726 442 Size: 958 1753 Fragments arranged by size: 1753 1282 958 653 416 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2476 2474 Size: 2 6035 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4697 1239 Size: 3458 2579 Bpu10I CC'TnA_GC Cuts at: 3059 3059 Size: 6037 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4162 5027 5325 4162 Size: 865 298 4874 Fragments arranged by size: 4874 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 2447 4700 882 Size: 617 588 360 2253 2219 Fragments arranged by size: 2253 2219 617 588 360 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 3071 6031 3071 Size: 2960 3077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3844 4226 4861 275 Size: 53 83 186 936 2311 382 635 1451 Fragments arranged by size: 2311 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4470 4868 5142 5289 5700 4470 Size: 398 274 147 411 4807 Fragments arranged by size: 4807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5812 Fragments arranged by size: 5812 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 1186 Size: 1003 5034 BseYI C'CCAG_C Cuts at: 2098 2344 5240 5736 2098 Size: 246 2896 496 2399 Fragments arranged by size: 2896 2399 496 246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2838 1163 Size: 573 414 688 4362 Fragments arranged by size: 4362 688 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3487 4248 5276 1540 Size: 275 186 1486 761 1028 2301 Fragments arranged by size: 2301 1486 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4098 4183 5254 4098 Size: 85 1071 4881 Fragments arranged by size: 4881 1071 85 BsmI GAATG_Cn' Cuts at: 3300 3548 3641 3300 Size: 248 93 5696 Fragments arranged by size: 5696 248 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2388 2424 2805 3188 3356 4098 4183 Size: 399 598 36 381 383 168 742 85 Cuts at: 4183 5254 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 598 399 383 381 168 85 36 BspEI T'CCGG_A Cuts at: 5700 5700 Size: 6037 BspHI T'CATG_A Cuts at: 2616 3270 3926 5656 2616 Size: 654 656 1730 2997 Fragments arranged by size: 2997 1730 656 654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3392 844 Size: 2548 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3924 1394 Size: 429 2101 3507 Fragments arranged by size: 3507 2101 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2599 4532 4706 65 Size: 1183 1351 1933 174 1396 Fragments arranged by size: 1933 1396 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3255 4681 4868 1700 Size: 110 1445 1426 187 2869 Fragments arranged by size: 2869 1445 1426 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 3776 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 2924 1184 Size: 1740 4297 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2899 2902 2962 3001 2011 Size: 888 3 60 39 5047 Fragments arranged by size: 5047 888 60 39 3 BstYI r'GATC_y Cuts at: 1972 2271 3367 3734 4119 4136 5588 5674 5686 Size: 299 1096 367 385 17 1452 86 12 Cuts at: 5686 6032 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1096 385 367 346 299 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5485 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5298 Fragments arranged by size: 5298 683 56 BtsI GCAGTG_nn' Cuts at: 3201 3553 4347 4367 3201 Size: 352 794 20 4871 Fragments arranged by size: 4871 794 352 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2325 3257 Size: 54 1425 50 275 18 381 114 932 Cuts at: 3257 3484 4373 6002 8 Size: 227 889 1629 43 Fragments arranged by size: 1629 1425 932 889 381 275 227 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3484 1537 Size: 275 1672 4090 Fragments arranged by size: 4090 1672 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2142 2187 3967 1353 Size: 789 45 1780 3423 Fragments arranged by size: 3423 1780 789 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4624 4998 5144 387 Size: 1064 138 3035 374 146 1280 Fragments arranged by size: 3035 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 4100 4697 5484 5752 1239 Size: 393 791 1677 597 787 268 1524 Fragments arranged by size: 1677 1524 791 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2438 2486 2045 Size: 393 48 5596 Fragments arranged by size: 5596 393 48 EcoO109I rG'GnC_Cy Cuts at: 2398 2398 Size: 6037 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 FspI TGC'GCA Cuts at: 4543 4543 Size: 6037 HaeII r_GCGC'y Cuts at: 1678 5184 1678 Size: 3506 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4678 4710 4752 4784 1373 Size: 32 135 32 3106 32 42 32 2626 Fragments arranged by size: 3106 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 2764 3381 3627 669 Size: 988 281 795 31 617 246 3079 Fragments arranged by size: 3079 988 795 617 281 246 31 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 2063 3362 2063 Size: 1299 4738 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3350 5151 5335 1305 Size: 2045 1801 184 2007 Fragments arranged by size: 2045 2007 1801 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 2327 10 Size: 54 1425 343 381 114 3720 Fragments arranged by size: 3720 1425 381 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2050 2798 2900 3248 3995 4354 Size: 648 843 41 748 102 348 747 359 Cuts at: 4354 4513 518 Size: 159 2042 Fragments arranged by size: 2042 843 748 747 648 359 348 159 102 41 MspA1I CmG'CkG Cuts at: 1606 3453 4130 5278 5523 1606 Size: 1847 677 1148 245 2120 Fragments arranged by size: 2120 1847 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5485 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NsiI A_TGCA'T Cuts at: 3051 3051 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2320 3395 4940 1132 Size: 97 1091 1075 1545 2229 Fragments arranged by size: 2229 1545 1091 1075 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6037 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2466 2498 4106 4138 5619 5651 2466 Size: 32 1608 32 1481 32 2852 Fragments arranged by size: 2852 1608 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PstI C_TGCA'G Cuts at: 830 3389 830 Size: 2559 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 SalI G'TCGA_C Cuts at: 1936 2762 3379 1936 Size: 826 617 4594 Fragments arranged by size: 4594 826 617 SbfI CC_TGCA'GG Cuts at: 3389 3389 Size: 6037 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SfcI C'TryA_G Cuts at: 826 1045 3385 4520 5201 5392 826 Size: 219 2340 1135 681 191 1471 Fragments arranged by size: 2340 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 2419 3364 1872 Size: 547 945 4545 Fragments arranged by size: 4545 945 547 SmlI C'TyrA_G Cuts at: 820 1017 1943 4141 5042 5304 820 Size: 197 926 2198 901 262 1553 Fragments arranged by size: 2198 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SrfI GCCC'GGGC Cuts at: 2419 2419 Size: 6037 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6037 StyI C'CwwG_G Cuts at: 513 1065 2139 2232 2322 2881 3476 513 Size: 552 1074 93 90 559 595 3074 Fragments arranged by size: 3074 1074 595 559 552 93 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 4064 4081 4234 4419 4864 325 Size: 1255 1332 1152 17 153 185 445 1498 Fragments arranged by size: 1498 1332 1255 1152 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4283 5995 Size: 274 80 33 51 1443 807 1499 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 1712 1499 1443 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 2747 1316 Size: 1431 4606 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2899 2962 2899 Size: 63 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2417 3362 1870 Size: 547 945 4545 Fragments arranged by size: 4545 945 547 XmnI GAAnn'nnTTC Cuts at: 4166 4166 Size: 6037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI