(Linear) (Six-base) MAPSORT of: KIEE6707.seq Check: 4017 from: 1 to: 3729 KIEE6707.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 307 3729 Size: 307 3422 AatII G_ACGT'C Cuts at: 0 2834 3729 Size: 2834 895 AccI GT'mk_AC Cuts at: 0 2864 2981 3389 3729 Size: 2864 117 408 340 Fragments arranged by size: 2864 408 340 117 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1927 3054 3729 Size: 1927 1127 675 Fragments arranged by size: 1927 1127 675 AfeI AGC'GCT Cuts at: 0 2488 3729 Size: 2488 1241 AhdI GACnn_n'nnGTC Cuts at: 0 1515 3729 Size: 1515 2214 AleI CACnn'nnGTG Cuts at: 0 2773 3729 Size: 2773 956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1028 3542 3729 Size: 1028 2514 187 Fragments arranged by size: 2514 1028 187 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2731 2763 3729 Size: 2731 32 966 Fragments arranged by size: 2731 966 32 AlwNI CAG_nnn'CTG Cuts at: 0 1174 1327 1365 2588 2883 3158 3327 3471 Size: 1174 153 38 1223 295 275 169 144 Cuts at: 3471 3729 Size: 258 Fragments arranged by size: 1223 1174 295 275 258 169 153 144 38 ApaI G_GGCC'C Cuts at: 0 155 554 666 1212 2825 3634 3658 3729 Size: 155 399 112 546 1613 809 24 71 Fragments arranged by size: 1613 809 546 399 155 112 71 24 ApoI r'AATT_y Cuts at: 0 316 3729 Size: 316 3413 AvaI C'yCGr_G Cuts at: 0 154 811 1016 1921 2215 2530 2928 3411 Size: 154 657 205 905 294 315 398 483 Cuts at: 3411 3534 3543 3624 3657 3729 Size: 123 9 81 33 72 Fragments arranged by size: 905 657 483 398 315 294 205 154 123 81 72 33 9 BamHI G'GATC_C Cuts at: 0 2897 3729 Size: 2897 832 BanI G'GyrC_C Cuts at: 0 856 1437 1588 1654 1744 2025 2367 2395 Size: 856 581 151 66 90 281 342 28 Cuts at: 2395 2908 3136 3729 Size: 513 228 593 Fragments arranged by size: 856 593 581 513 342 281 228 151 90 66 28 BanII G_rGCy'C Cuts at: 0 155 199 554 666 1212 1372 1492 1922 Size: 155 44 355 112 546 160 120 430 Cuts at: 1922 1930 1979 2222 2825 3517 3634 3658 3729 Size: 8 49 243 603 692 117 24 71 Fragments arranged by size: 692 603 546 430 355 243 160 155 120 117 112 71 49 44 24 8 BbeI G_GCGC'C Cuts at: 0 1592 3140 3729 Size: 1592 1548 589 Fragments arranged by size: 1592 1548 589 BbsI GAAGACnn'nnnn_ Cuts at: 0 366 1080 3060 3729 Size: 366 714 1980 669 Fragments arranged by size: 1980 714 669 366 BbvCI CC'TCA_GC Cuts at: 0 1262 3729 Size: 1262 2467 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3218 3252 3729 Size: 3218 34 477 Fragments arranged by size: 3218 477 34 BglI GCCn_nnn'nGGC Cuts at: 0 1381 2143 3729 Size: 1381 762 1586 Fragments arranged by size: 1586 1381 762 BglII A'GATC_T Cuts at: 0 2171 3729 Size: 2171 1558 BlpI GC'TnA_GC Cuts at: 0 706 1366 2800 3729 Size: 706 660 1434 929 Fragments arranged by size: 1434 929 706 660 Bme1580I G_kGCm'C Cuts at: 0 155 225 531 554 666 729 1212 1442 Size: 155 70 306 23 112 63 483 230 Cuts at: 1442 1747 2267 2372 2825 3634 3658 3729 Size: 305 520 105 453 809 24 71 Fragments arranged by size: 809 520 483 453 306 305 230 155 112 105 71 70 63 24 23 BmgBI CAC'GTC Cuts at: 0 2604 3147 3729 Size: 2604 543 582 Fragments arranged by size: 2604 582 543 BmrI ACTGGGnnnn_n' Cuts at: 0 534 606 1141 1807 2107 2598 2941 3729 Size: 534 72 535 666 300 491 343 788 Fragments arranged by size: 788 666 535 534 491 343 300 72 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 336 1050 1812 3729 Size: 336 714 762 1917 Fragments arranged by size: 1917 762 714 336 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1349 2161 2319 2368 2377 2503 2867 Size: 1196 153 812 158 49 9 126 364 Cuts at: 2867 3729 Size: 862 Fragments arranged by size: 1196 862 812 364 158 153 126 49 9 Bpu10I CC'TnA_GC Cuts at: 0 382 1262 1486 1916 3729 Size: 382 880 224 430 1813 Fragments arranged by size: 1813 880 430 382 224 BsaI GGTCTCn'nnnn_ Cuts at: 0 328 2073 3374 3729 Size: 328 1745 1301 355 Fragments arranged by size: 1745 1301 355 328 BsaAI yAC'GTr Cuts at: 0 192 1422 3729 Size: 192 1230 2307 Fragments arranged by size: 2307 1230 192 BsaHI Gr'CG_yC Cuts at: 0 1334 1589 2831 3137 3260 3729 Size: 1334 255 1242 306 123 469 Fragments arranged by size: 1334 1242 469 306 255 123 BsaWI w'CCGG_w Cuts at: 0 1800 2735 3729 Size: 1800 935 994 Fragments arranged by size: 1800 994 935 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 181 211 956 986 1324 1354 1679 1709 Size: 181 30 745 30 338 30 325 30 Cuts at: 1709 2476 2506 2840 2870 3729 Size: 767 30 334 30 859 Fragments arranged by size: 859 767 745 338 334 325 181 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 106 411 751 1096 1956 2365 2680 3522 Size: 106 305 340 345 860 409 315 842 Cuts at: 3522 3729 Size: 207 Fragments arranged by size: 860 842 409 345 340 315 305 207 106 BseYI C'CCAG_C Cuts at: 0 59 758 803 1254 1886 1897 2038 2157 Size: 59 699 45 451 632 11 141 119 Cuts at: 2157 2438 2452 2967 3399 3634 3729 Size: 281 14 515 432 235 95 Fragments arranged by size: 699 632 515 451 432 281 235 141 119 95 59 45 14 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1282 1378 3352 3729 Size: 1282 96 1974 377 Fragments arranged by size: 1974 1282 377 96 BsiEI CG_ry'CG Cuts at: 0 2195 3202 3729 Size: 2195 1007 527 Fragments arranged by size: 2195 1007 527 BsiHKAI G_wGCw'C Cuts at: 0 826 1372 3729 Size: 826 546 2357 Fragments arranged by size: 2357 826 546 BsmI GAATG_Cn' Cuts at: 0 273 2665 3031 3729 Size: 273 2392 366 698 Fragments arranged by size: 2392 698 366 273 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1325 3105 3270 3729 Size: 1325 1780 165 459 Fragments arranged by size: 1780 1325 459 165 Bsp1286I G_dGCh'C Cuts at: 0 155 199 225 531 554 666 729 826 Size: 155 44 26 306 23 112 63 97 Cuts at: 826 1212 1372 1442 1492 1747 1922 1930 1979 Size: 386 160 70 50 255 175 8 49 Cuts at: 1979 2222 2267 2372 2825 3517 3634 3658 3729 Size: 243 45 105 453 692 117 24 71 Fragments arranged by size: 692 453 386 306 255 243 175 160 155 117 112 105 97 71 70 63 50 49 45 44 26 24 23 8 BspEI T'CCGG_A Cuts at: 0 1800 2735 3729 Size: 1800 935 994 Fragments arranged by size: 1800 994 935 BspHI T'CATG_A Cuts at: 0 2870 3729 Size: 2870 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 237 307 353 1662 2715 3729 Size: 237 70 46 1309 1053 1014 Fragments arranged by size: 1309 1053 1014 237 70 46 BsrBI CCG'CTC Cuts at: 0 386 2643 3729 Size: 386 2257 1086 Fragments arranged by size: 2257 1086 386 BsrDI GCAATG_nn' Cuts at: 0 1010 1604 3317 3729 Size: 1010 594 1713 412 Fragments arranged by size: 1713 1010 594 412 BsrFI r'CCGG_y Cuts at: 0 134 1657 3197 3358 3729 Size: 134 1523 1540 161 371 Fragments arranged by size: 1540 1523 371 161 134 BssHII G'CGCG_C Cuts at: 0 2963 3729 Size: 2963 766 BstXI CCAn_nnnn'nTGG Cuts at: 0 39 1905 2469 3381 3678 3729 Size: 39 1866 564 912 297 51 Fragments arranged by size: 1866 912 564 297 51 39 BstYI r'GATC_y Cuts at: 0 122 2171 2477 2732 2897 3729 Size: 122 2049 306 255 165 832 Fragments arranged by size: 2049 832 306 255 165 122 BstZ17I GTA'TAC Cuts at: 0 3390 3729 Size: 3390 339 Bsu36I CC'TnA_GG Cuts at: 0 872 1406 1649 3729 Size: 872 534 243 2080 Fragments arranged by size: 2080 872 534 243 BtgI C'CryG_G Cuts at: 0 1592 1770 3048 3140 3255 3587 3729 Size: 1592 178 1278 92 115 332 142 Fragments arranged by size: 1592 1278 332 178 142 115 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2232 3729 Size: 2232 1497 BtsI GCAGTG_nn' Cuts at: 0 223 446 792 928 1555 2045 2418 3466 Size: 223 223 346 136 627 490 373 1048 Cuts at: 3466 3729 Size: 263 Fragments arranged by size: 1048 627 490 373 346 263 223 223 136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1587 1622 3729 Size: 1587 35 2107 Fragments arranged by size: 2107 1587 35 DraIII CAC_nnn'GTG Cuts at: 0 183 3222 3729 Size: 183 3039 507 Fragments arranged by size: 3039 507 183 DrdI GACnn_nn'nnGTC Cuts at: 0 2065 3729 Size: 2065 1664 EaeI y'GGCC_r Cuts at: 0 128 767 842 1562 1595 1907 2192 2312 Size: 128 639 75 720 33 312 285 120 Cuts at: 2312 2420 2644 3199 3356 3729 Size: 108 224 555 157 373 Fragments arranged by size: 720 639 555 373 312 285 224 157 128 120 108 75 33 EagI C'GGCC_G Cuts at: 0 2192 3199 3729 Size: 2192 1007 530 Fragments arranged by size: 2192 1007 530 EarI CTCTTCn'nnn_ Cuts at: 0 248 461 728 1037 1291 2089 2188 2578 Size: 248 213 267 309 254 798 99 390 Cuts at: 2578 3729 Size: 1151 Fragments arranged by size: 1151 798 390 309 267 254 248 213 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1362 1366 2586 2671 3658 3729 Size: 1362 4 1220 85 987 71 Fragments arranged by size: 1362 1220 987 85 71 4 EcoICRI GAG'CTC Cuts at: 0 1370 3729 Size: 1370 2359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1349 1927 2161 2319 2368 2377 2503 Size: 1196 153 578 234 158 49 9 126 Cuts at: 2503 2867 3054 3729 Size: 364 187 675 Fragments arranged by size: 1196 675 578 364 234 187 158 153 126 49 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 2623 2694 3331 3729 Size: 2623 71 637 398 Fragments arranged by size: 2623 637 398 71 EcoO109I rG'GnC_Cy Cuts at: 0 471 550 551 876 1208 1233 1324 1821 Size: 471 79 1 325 332 25 91 497 Cuts at: 1821 2822 3538 3630 3631 3654 3729 Size: 1001 716 92 1 23 75 Fragments arranged by size: 1001 716 497 471 332 325 92 91 79 75 25 23 1 1 FseI GG_CCGG'CC Cuts at: 0 3201 3729 Size: 3201 528 FspI TGC'GCA Cuts at: 0 1272 2119 2992 3601 3729 Size: 1272 847 873 609 128 Fragments arranged by size: 1272 873 847 609 128 FspAI rTGC'GCAy Cuts at: 0 2992 3729 Size: 2992 737 HaeII r_GCGC'y Cuts at: 0 1429 1592 2490 2539 2811 3140 3400 3729 Size: 1429 163 898 49 272 329 260 329 Fragments arranged by size: 1429 898 329 329 272 260 163 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 392 424 820 852 1324 1356 1501 1533 Size: 392 32 396 32 472 32 145 32 Cuts at: 1533 2284 2316 2656 2688 3729 Size: 751 32 340 32 1041 Fragments arranged by size: 1041 751 472 396 392 340 145 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1588 3136 3729 Size: 1588 1548 593 Fragments arranged by size: 1588 1548 593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1505 2774 3729 Size: 1505 1269 955 Fragments arranged by size: 1505 1269 955 MscI TGG'CCA Cuts at: 0 130 769 1564 1597 1909 2422 3729 Size: 130 639 795 33 312 513 1307 Fragments arranged by size: 1307 795 639 513 312 130 33 MslI CAynn'nnrTG Cuts at: 0 2773 3017 3524 3729 Size: 2773 244 507 205 Fragments arranged by size: 2773 507 244 205 MspA1I CmG'CkG Cuts at: 0 113 164 1304 2011 2652 2883 3050 3504 Size: 113 51 1140 707 641 231 167 454 Cuts at: 3504 3615 3729 Size: 111 114 Fragments arranged by size: 1140 707 641 454 231 167 114 113 111 51 NaeI GCC'GGC Cuts at: 0 1659 3199 3729 Size: 1659 1540 530 Fragments arranged by size: 1659 1540 530 NarI GG'CG_CC Cuts at: 0 1589 3137 3729 Size: 1589 1548 592 Fragments arranged by size: 1589 1548 592 NgoMIV G'CCGG_C Cuts at: 0 1657 3197 3729 Size: 1657 1540 532 Fragments arranged by size: 1657 1540 532 NspI r_CATG'y Cuts at: 0 606 753 1005 1271 3529 3729 Size: 606 147 252 266 2258 200 Fragments arranged by size: 2258 606 266 252 200 147 PasI CC'CwG_GG Cuts at: 0 1220 2286 2696 3729 Size: 1220 1066 410 1033 Fragments arranged by size: 1220 1066 1033 410 PflMI CCAn_nnn'nTGG Cuts at: 0 765 1174 2588 3729 Size: 765 409 1414 1141 Fragments arranged by size: 1414 1141 765 409 PfoI T'CCnGG_A Cuts at: 0 262 410 1313 2480 3729 Size: 262 148 903 1167 1249 Fragments arranged by size: 1249 1167 903 262 148 PmlI CAC'GTG Cuts at: 0 192 1422 3729 Size: 192 1230 2307 Fragments arranged by size: 2307 1230 192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1030 1062 3729 Size: 1030 32 2667 Fragments arranged by size: 2667 1030 32 PpuMI rG'GwC_Cy Cuts at: 0 471 1821 3538 3729 Size: 471 1350 1717 191 Fragments arranged by size: 1717 1350 471 191 PspOMI G'GGCC_C Cuts at: 0 151 550 662 1208 2821 3630 3654 3729 Size: 151 399 112 546 1613 809 24 75 Fragments arranged by size: 1613 809 546 399 151 112 75 24 PspXI vC'TCGA_Gb Cuts at: 0 2215 3624 3729 Size: 2215 1409 105 Fragments arranged by size: 2215 1409 105 PstI C_TGCA'G Cuts at: 0 248 2327 3323 3729 Size: 248 2079 996 406 Fragments arranged by size: 2079 996 406 248 PvuII CAG'CTG Cuts at: 0 2652 3504 3615 3729 Size: 2652 852 111 114 Fragments arranged by size: 2652 852 114 111 SacI G_AGCT'C Cuts at: 0 1372 3729 Size: 1372 2357 SacII CC_GC'GG Cuts at: 0 3051 3729 Size: 3051 678 SanDI GG'GwC_CC Cuts at: 0 1821 3538 3729 Size: 1821 1717 191 Fragments arranged by size: 1821 1717 191 SbfI CC_TGCA'GG Cuts at: 0 248 3729 Size: 248 3481 SexAI A'CCwGG_T Cuts at: 0 50 3729 Size: 50 3679 SfcI C'TryA_G Cuts at: 0 244 2323 2679 3319 3451 3729 Size: 244 2079 356 640 132 278 Fragments arranged by size: 2079 640 356 278 244 132 SfoI GGC'GCC Cuts at: 0 1590 3138 3729 Size: 1590 1548 591 Fragments arranged by size: 1590 1548 591 SmaI CCC'GGG Cuts at: 0 156 813 3659 3729 Size: 156 657 2846 70 Fragments arranged by size: 2846 657 156 70 SmlI C'TyrA_G Cuts at: 0 2215 3624 3729 Size: 2215 1409 105 Fragments arranged by size: 2215 1409 105 SphI G_CATG'C Cuts at: 0 606 753 1005 1271 3529 3729 Size: 606 147 252 266 2258 200 Fragments arranged by size: 2258 606 266 252 200 147 StuI AGG'CCT Cuts at: 0 676 1411 1624 1847 3729 Size: 676 735 213 223 1882 Fragments arranged by size: 1882 735 676 223 213 StyI C'CwwG_G Cuts at: 0 233 1094 1412 3122 3554 3729 Size: 233 861 318 1710 432 175 Fragments arranged by size: 1710 861 432 318 233 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 480 1233 1464 2394 3301 3684 3729 Size: 480 753 231 930 907 383 45 Fragments arranged by size: 930 907 753 480 383 231 45 TatI w'GTAC_w Cuts at: 0 455 2522 3729 Size: 455 2067 1207 Fragments arranged by size: 2067 1207 455 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 181 213 956 988 2397 2429 2476 2508 Size: 181 32 743 32 1409 32 47 32 Cuts at: 2508 2532 2564 2840 2872 3729 Size: 24 32 276 32 857 Fragments arranged by size: 1409 857 743 276 181 47 32 32 32 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 0 2833 3148 3729 Size: 2833 315 581 Fragments arranged by size: 2833 581 315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2343 2466 3729 Size: 2343 123 1263 Fragments arranged by size: 2343 1263 123 XhoI C'TCGA_G Cuts at: 0 2215 3624 3729 Size: 2215 1409 105 Fragments arranged by size: 2215 1409 105 XmaI C'CCGG_G Cuts at: 0 154 811 3657 3729 Size: 154 657 2846 72 Fragments arranged by size: 2846 657 154 72 ZraI GAC'GTC Cuts at: 0 2832 3729 Size: 2832 897 Enzymes that do cut: AarI AatII AccI AcuI AfeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SbfI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AflII AflIII AgeI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BpuEI BsaBI BsiWI BsrGI BssSI BstAPI BstBI BstEII ClaI DraI EcoRI EcoRV FalI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII SalI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XmnI