################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 209 CGCG CG^CG AcsI 99 GAATTC [GA]^AATT[CT] ApoI 99 GAATTC [GA]^AATT[CT] AspHI 11 GTGCTC G[AT]GC[AT]^C AvaI 269 CCCGGG C^[CT]CG[GA]G AvaII 30 GGTCC G^G[AT]CC BanII 138 GAGCCC G[GA]GC[CT]^C BbvCI 143 CCTCAGC CCTCAGC(-5/-2) BglI 197 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 251 AGATCT A^GATCT BpmI 259 CTGGAG CTGGAG(16/14) BsiHKAI 11 GTGCTC G[AT]GC[AT]^C BsoBI 269 CCCGGG C^[CT]CG[GA]G BspMI 123 ACCTGC ACCTGC(4/8) BstUI 209 CGCG CG^CG BstYI 251 AGATCT [GA]^GATC[CT] CfoI 210 GCGC GCG^C DpnII 252 GATC ^GATC DsaI 163 CCGTGG C^C[GA][CT]GG EcoRI 99 GAATTC G^AATTC HapII 270 CCGG C^CGG HhaI 210 GCGC GCG^C HinP1I 210 GCGC G^CGC HinfI 47 GAGTC G^A[ACGT]TC HpaII 270 CCGG C^CGG MaeIII 64 GTGAC ^GT[ACGT]AC MboI 252 GATC ^GATC MflI 251 AGATCT [GA]^GATC[CT] MslI 158 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 146 CAGCTG C[AC]G^C[GT]G MspI 270 CCGG C^CGG MvnI 209 CGCG CG^CG NdeII 252 GATC ^GATC NlaIII 158 CATG CATG^ PleI 47 GAGTC GAGTC(4/5) PstI 183 CTGCAG CTGCA^G PvuII 146 CAGCTG CAG^CTG Sau3AI 252 GATC ^GATC SexAI 4 ACCAGGT A^CC[AT]GGT SfaNI 311 GCATC GCATC(5/9) SfcI 183 CTGCAG C^T[GA][CT]AG SmaI 269 CCCGGG CCC^GGG Tsp45I 64 GTGAC ^GT[GC]AC Tsp509I 100 AATT ^AATT XhoII 251 AGATCT [GA]^GATC[CT] XmaCI 269 CCCGGG C^CCGGG XmaI 269 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 86 AGCT AG^CT 147 AGCT BcnI 269 CCCGG CC^[GC]GG 270 CCGGG BmyI 11 GTGCTC G[AGT]GC[ACT]^C 138 GAGCCC BsmFI 58 GTCCC GGGAC(10/14) 232 GTCCC Bsp1286I 11 GTGCTC G[AGT]GC[ACT]^C 138 GAGCCC BsrI 258 ACTGG ACTGG(1/-1) 61 CCAGT BstF5I 312 CATCC GGATG(2/0) 228 GGATG EaeI 160 TGGCCG [CT]^GGCC[GA] 327 CGGCCA FauI 194 CCCGC CCCGC(4/6) 308 CCCGC FokI 312 CATCC GGATG(9/13) 228 GGATG NciI 269 CCCGG CC^[GC]GG 270 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI Sau96I ScrFI TseI TspRI