################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 946 GTCTAC GT^[AC][GT]AC AflIII 494 ACATGT A^C[GA][CT]GT AlwI 695 GGATC GGATC(4/5) Asp700I 72 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 595 GAGCTC G[AT]GC[AT]^C BalI 473 TGGCCA TGG^CCA BanII 595 GAGCTC G[GA]GC[CT]^C BclI 299 TGATCA T^GATCA BcnI 650 CCCGG CC^[GC]GG BglI 544 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 807 GCTGAGC GC^T[ACGT]AGC BpmI 319 CTCCAG CTGGAG(16/14) Bpu1102I 807 GCTGAGC GC^T[ACGT]AGC BsaI 961 GGTCTC GGTCTC(1/5) BsgI 784 CTGCAC GTGCAG(16/14) BsiHKAI 595 GAGCTC G[AT]GC[AT]^C BsmAI 962 GTCTC GTCTC(1/5) BsmFI 44 GGGAC GGGAC(10/14) BsmI 405 GAATGC GAATGC(1/-1) BspDI 28 ATCGAT AT^CGAT BspLU11I 494 ACATGT A^CATGT BspMI 798 GCAGGT ACCTGC(4/8) BsrDI 794 CATTGC GCAATG(2/0) BsrFI 339 GCCGGC [GA]^CCGG[CT] CelII 807 GCTGAGC GC^T[ACGT]AGC Cfr10I 339 GCCGGC [GA]^CCGG[CT] ClaI 28 ATCGAT AT^CGAT DraII 779 AGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 476 CCACGG C^C[GA][CT]GG EarI 153 GAAGAG CTCTTC(1/4) EcoO109I 779 AGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 299 TGATCA T^GATCA HaeII 510 AGCGCC [GA]GCGC^[CT] Ksp632I 153 GAAGAG CTCTTC(1/4) MluNI 473 TGGCCA TGG^CCA MscI 473 TGGCCA TGG^CCA MseI 23 TTAA T^TAA NaeI 339 GCCGGC GCC^GGC NciI 650 CCCGG CC^[GC]GG NgoMIV 339 GCCGGC G^CCGGC PleI 223 GACTC GAGTC(4/5) PpuMI 779 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 196 CAGCTG CAG^CTG SacI 595 GAGCTC GAGCT^C SapI 153 GAAGAGC GCTCTTC(1/4) SfcI 327 CTACAG C^T[GA][CT]AG TaqI 29 TCGA T^CGA TfiI 608 GAATC G^A[AT]TC Tru9I 23 TTAA T^TAA TthHB8I 29 TCGA T^CGA XmnI 72 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 196 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 707 CAGCTCCTG BsrBI 118 GAGCGG CCGCTC(-3/-3) 810 GAGCGG BstAPI 759 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 798 GCAGGTCATGC BstF5I 161 GGATG GGATG(2/0) 716 GGATG Cac8I 339 GCCGGC GC[ACGT]^[ACGT]GC 786 GCACGC EaeI 473 TGGCCA [CT]^GGCC[GA] 662 CGGCCA EcoT14I 137 CCAAGG C^C[AT][AT]GG 518 CCTTGG FokI 161 GGATG GGATG(9/13) 716 GGATG HapII 340 CCGG C^CGG 651 CCGG HincII 388 GTCAAC GT[CT]^[GA]AC 498 GTTGAC HindII 388 GTCAAC GT[CT]^[GA]AC 498 GTTGAC HinfI 223 GACTC G^A[ACGT]TC 608 GAATC HpaII 340 CCGG C^CGG 651 CCGG MnlI 822 CCTC CCTC(7/6) 4 GAGG MspI 340 CCGG C^CGG 651 CCGG NspI 272 GCATGT [GA]CATG^[CT] 494 ACATGT SmlI 748 CTTGAG C^T[CT][GA]AG 934 CTTGAG StyI 137 CCAAGG C^C[AT][AT]GG 518 CCTTGG Tsp509I 755 AATT ^AATT 872 AATT XcmI 137 CCAAGGTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 234 CCACCCCGACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AvaII BanI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstXI CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeIII HhaI HinP1I HphI ItaI MboI MboII MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI TspRI