(Circular) (Six-base) MAPSORT of: pFN21AE6306.seq Check: 3572 from: 1 to: 5671 pFN21AE6306 5671 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2952 3014 1937 Size: 1015 62 4594 Fragments arranged by size: 4594 1015 62 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3734 5118 5386 1632 Size: 2102 1384 268 1917 Fragments arranged by size: 2102 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 2499 4570 5547 1203 Size: 1296 2071 977 1327 Fragments arranged by size: 2071 1327 1296 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 2192 2474 2486 1166 Size: 1026 282 12 4351 Fragments arranged by size: 4351 1026 282 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2889 2921 2889 Size: 32 5639 AlwNI CAG_nnn'CTG Cuts at: 2206 2717 3414 4986 2206 Size: 511 697 1572 2891 Fragments arranged by size: 2891 1572 697 511 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2980 3206 3358 5467 1493 Size: 1487 226 152 2109 1697 Fragments arranged by size: 2109 1697 1487 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2996 1870 Size: 73 1053 4545 Fragments arranged by size: 4545 1053 73 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2186 2539 2839 2992 4447 Size: 325 217 627 399 353 300 153 1455 Cuts at: 4447 618 Size: 1842 Fragments arranged by size: 1842 1455 627 399 353 325 300 217 153 BanII G_rGCy'C Cuts at: 1391 2604 2990 1391 Size: 1213 386 4072 Fragments arranged by size: 4072 1213 386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 2304 1548 Size: 756 4915 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2555 4282 Size: 107 122 71 631 605 337 546 1727 Cuts at: 4282 136 Size: 1525 Fragments arranged by size: 1727 1525 631 605 546 337 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 2813 3088 2813 Size: 275 5396 Bme1580I G_kGCm'C Cuts at: 1790 2181 2544 3732 4888 1790 Size: 391 363 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 391 363 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2146 2259 3402 Size: 416 53 83 399 24 729 113 1143 Cuts at: 3402 4360 442 Size: 958 1753 Fragments arranged by size: 1753 1143 958 729 416 399 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 4331 1239 Size: 1070 2022 2579 Fragments arranged by size: 2579 2022 1070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 2960 3796 4661 4959 2774 Size: 186 836 865 298 3486 Fragments arranged by size: 3486 865 836 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2972 4334 882 Size: 617 1473 1362 2219 Fragments arranged by size: 2219 1473 1362 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2889 2919 1178 Size: 30 165 30 1486 30 3930 Fragments arranged by size: 3930 1486 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 2535 4874 5370 2535 Size: 2339 496 2836 Fragments arranged by size: 2836 2339 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2774 1163 Size: 573 1038 4060 Fragments arranged by size: 4060 1038 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2604 3732 3817 4888 2604 Size: 1128 85 1071 3387 Fragments arranged by size: 3387 1128 1071 85 BsmI GAATG_Cn' Cuts at: 2416 3182 3275 2416 Size: 766 93 4812 Fragments arranged by size: 4812 766 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2544 2604 2990 3732 3817 4888 Size: 399 391 363 60 386 742 85 1071 Cuts at: 4888 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 391 386 363 85 60 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2794 3026 844 Size: 1950 232 3489 Fragments arranged by size: 3489 1950 232 BsrBI CCG'CTC Cuts at: 1394 1823 2125 2817 3558 1394 Size: 429 302 692 741 3507 Fragments arranged by size: 3507 741 692 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 2798 4166 4340 65 Size: 1183 1550 1368 174 1396 Fragments arranged by size: 1550 1396 1368 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2344 4315 4502 1700 Size: 110 534 1971 187 2869 Fragments arranged by size: 2869 1971 534 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 2770 2809 794 Size: 1976 39 3656 Fragments arranged by size: 3656 1976 39 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2294 2476 2488 2149 Size: 145 182 12 5332 Fragments arranged by size: 5332 182 145 12 BstYI r'GATC_y Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666 Size: 1029 367 385 17 1452 86 12 346 Cuts at: 5666 1972 Size: 1977 Fragments arranged by size: 1977 1452 1029 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 513 Size: 552 939 477 3703 Fragments arranged by size: 3703 939 552 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2369 2469 3187 3981 4001 2369 Size: 100 718 794 20 4039 Fragments arranged by size: 4039 794 718 100 20 ClaI AT'CG_AT Cuts at: 1554 2034 1554 Size: 480 5191 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2478 2667 3118 Size: 54 1425 50 275 18 648 189 451 Cuts at: 3118 4007 5636 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 648 451 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 3601 1353 Size: 800 1448 3423 Fragments arranged by size: 3423 1448 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4258 4632 4778 387 Size: 1064 138 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2602 2602 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 3734 4331 5118 5386 1239 Size: 393 677 1425 597 787 268 1524 Fragments arranged by size: 1524 1425 787 677 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2785 2785 Size: 5671 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2519 4818 1678 Size: 841 2299 2531 Fragments arranged by size: 2531 2299 841 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373 Size: 32 135 32 2740 32 42 32 2626 Fragments arranged by size: 2740 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2395 2505 3015 3261 669 Size: 988 281 457 110 510 246 3079 Fragments arranged by size: 3079 988 510 457 281 246 110 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2480 10 Size: 54 1425 343 648 3201 Fragments arranged by size: 3201 1425 648 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2474 2486 2498 2834 2869 3629 Size: 648 1026 282 12 12 336 35 760 Cuts at: 3629 3988 4147 518 Size: 359 159 2042 Fragments arranged by size: 2042 1026 760 648 359 336 282 159 35 12 12 MspA1I CmG'CkG Cuts at: 1606 2203 2535 2552 3087 3764 4912 5157 1606 Size: 597 332 17 535 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 597 535 332 245 17 NaeI GCC'GGC Cuts at: 2346 2346 Size: 5671 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4180 Fragments arranged by size: 4180 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NgoMIV G'CCGG_C Cuts at: 2344 2344 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2281 2503 3029 4574 1132 Size: 97 1052 222 526 1545 2229 Fragments arranged by size: 2229 1545 1052 526 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 2499 4570 2499 Size: 2071 3600 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PfoI T'CCnGG_A Cuts at: 2654 2654 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2889 2921 3740 3772 5253 5285 2889 Size: 32 819 32 1481 32 3275 Fragments arranged by size: 3275 1481 819 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2785 2785 Size: 5671 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 3023 830 Size: 2193 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2203 2203 Size: 5671 SacI G_AGCT'C Cuts at: 2604 2604 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SapI GCTCTTCn'nnn_ Cuts at: 2153 2153 Size: 5671 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2332 3019 4154 4835 5026 826 Size: 219 1287 687 1135 681 191 1471 Fragments arranged by size: 1471 1287 1135 687 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 2939 3775 4676 4938 820 Size: 197 926 810 186 836 901 262 1553 Fragments arranged by size: 1553 926 901 836 810 262 197 186 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5671 StyI C'CwwG_G Cuts at: 513 1065 2004 2142 2523 3110 513 Size: 552 939 138 381 587 3074 Fragments arranged by size: 3074 939 587 552 381 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2356 2527 2696 3698 3715 3868 Size: 1255 624 152 171 169 1002 17 153 Cuts at: 3868 4053 4498 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1002 624 445 185 171 169 153 152 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2604 2636 5631 5663 2604 Size: 32 2995 32 2612 Fragments arranged by size: 2995 2612 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2246 2149 Size: 97 5574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 2081 3800 2081 Size: 1719 3952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI