(Linear) (Six-base) MAPSORT of: KIEE6306.seq Check: 9084 from: 1 to: 966 KIEE6306.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 947 966 Size: 947 19 AflIII A'CryG_T Cuts at: 0 494 966 Size: 494 472 AleI CACnn'nnGTG Cuts at: 0 187 469 481 966 Size: 187 282 12 485 Fragments arranged by size: 485 282 187 12 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 884 916 966 Size: 884 32 50 Fragments arranged by size: 884 50 32 AlwNI CAG_nnn'CTG Cuts at: 0 201 712 966 Size: 201 511 254 Fragments arranged by size: 511 254 201 BanI G'GyrC_C Cuts at: 0 181 534 834 966 Size: 181 353 300 132 Fragments arranged by size: 353 300 181 132 BanII G_rGCy'C Cuts at: 0 599 966 Size: 599 367 BclI T'GATC_A Cuts at: 0 299 966 Size: 299 667 BglI GCCn_nnn'nGGC Cuts at: 0 550 966 Size: 550 416 BlpI GC'TnA_GC Cuts at: 0 808 966 Size: 808 158 Bme1580I G_kGCm'C Cuts at: 0 176 539 966 Size: 176 363 427 Fragments arranged by size: 427 363 176 BmrI ACTGGGnnnn_n' Cuts at: 0 141 254 966 Size: 141 113 712 Fragments arranged by size: 712 141 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 966 Size: 304 662 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 955 966 Size: 769 186 11 Fragments arranged by size: 769 186 11 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 884 914 966 Size: 884 30 52 Fragments arranged by size: 884 52 30 BseYI C'CCAG_C Cuts at: 0 530 966 Size: 530 436 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 769 966 Size: 769 197 BsiHKAI G_wGCw'C Cuts at: 0 599 966 Size: 599 367 BsmI GAATG_Cn' Cuts at: 0 411 966 Size: 411 555 Bsp1286I G_dGCh'C Cuts at: 0 176 539 599 966 Size: 176 363 60 367 Fragments arranged by size: 367 363 176 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 789 966 Size: 789 177 BsrBI CCG'CTC Cuts at: 0 120 812 966 Size: 120 692 154 Fragments arranged by size: 692 154 120 BsrDI GCAATG_nn' Cuts at: 0 793 966 Size: 793 173 BsrFI r'CCGG_y Cuts at: 0 339 966 Size: 339 627 BstAPI GCAn_nnn'nTGC Cuts at: 0 765 804 966 Size: 765 39 162 Fragments arranged by size: 765 162 39 BstXI CCAn_nnnn'nTGG Cuts at: 0 144 289 471 483 966 Size: 144 145 182 12 483 Fragments arranged by size: 483 182 145 144 12 BtgI C'CryG_G Cuts at: 0 476 966 Size: 476 490 BtsI GCAGTG_nn' Cuts at: 0 364 464 966 Size: 364 100 502 Fragments arranged by size: 502 364 100 ClaI AT'CG_AT Cuts at: 0 29 966 Size: 29 937 EaeI y'GGCC_r Cuts at: 0 473 662 966 Size: 473 189 304 Fragments arranged by size: 473 304 189 EarI CTCTTCn'nnn_ Cuts at: 0 148 966 Size: 148 818 EcoICRI GAG'CTC Cuts at: 0 597 966 Size: 597 369 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 966 Size: 304 662 EcoO109I rG'GnC_Cy Cuts at: 0 780 966 Size: 780 186 HaeII r_GCGC'y Cuts at: 0 514 966 Size: 514 452 HincII GTy'rAC Cuts at: 0 390 500 966 Size: 390 110 466 Fragments arranged by size: 466 390 110 MscI TGG'CCA Cuts at: 0 475 966 Size: 475 491 MslI CAynn'nnrTG Cuts at: 0 187 469 481 493 829 864 966 Size: 187 282 12 12 336 35 102 Fragments arranged by size: 336 282 187 102 35 12 12 MspA1I CmG'CkG Cuts at: 0 198 530 547 966 Size: 198 332 17 419 Fragments arranged by size: 419 332 198 17 NaeI GCC'GGC Cuts at: 0 341 966 Size: 341 625 NgoMIV G'CCGG_C Cuts at: 0 339 966 Size: 339 627 NspI r_CATG'y Cuts at: 0 276 498 966 Size: 276 222 468 Fragments arranged by size: 468 276 222 PciI A'CATG_T Cuts at: 0 494 966 Size: 494 472 PfoI T'CCnGG_A Cuts at: 0 649 966 Size: 649 317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 884 916 966 Size: 884 32 50 Fragments arranged by size: 884 50 32 PpuMI rG'GwC_Cy Cuts at: 0 780 966 Size: 780 186 PvuII CAG'CTG Cuts at: 0 198 966 Size: 198 768 SacI G_AGCT'C Cuts at: 0 599 966 Size: 599 367 SapI GCTCTTCn'nnn_ Cuts at: 0 148 966 Size: 148 818 SfcI C'TryA_G Cuts at: 0 327 966 Size: 327 639 SmlI C'TyrA_G Cuts at: 0 748 934 966 Size: 748 186 32 Fragments arranged by size: 748 186 32 StyI C'CwwG_G Cuts at: 0 137 518 966 Size: 137 381 448 Fragments arranged by size: 448 381 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 199 351 522 691 966 Size: 199 152 171 169 275 Fragments arranged by size: 275 199 171 169 152 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 599 631 966 Size: 599 32 335 Fragments arranged by size: 599 335 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 144 241 966 Size: 144 97 725 Fragments arranged by size: 725 144 97 XmnI GAAnn'nnTTC Cuts at: 0 76 966 Size: 76 890 Enzymes that do cut: AccI AflIII AleI AloI AlwNI BanI BanII BclI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HincII MscI MslI MspA1I NaeI NgoMIV NspI PciI PfoI PpiI PpuMI PvuII SacI SapI SfcI SmlI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI