################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AvrII BbeI BbrPI BbsI BbvCI BcgI BclI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsoBI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FbaI FseI HaeII Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 2310 GTCTAC GT^[AC][GT]AC AccII 80 CGCG CG^CG AflII 1551 CTTAAG C^TTAAG AhdI 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2317 GAGCTC G[AT]GC[AT]^C AviII 932 TGCGCA TGC^GCA BciVI 963 GTATCC GTATCC(6/5) BcnI 1911 CCGGG CC^[GC]GG BfrI 1551 CTTAAG C^TTAAG BglI 2035 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2770 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2770 GCTCAGC GC^T[ACGT]AGC BsaWI 2071 ACCGGA [AT]^CCGG[AT] BsiHKAI 2317 GAGCTC G[AT]GC[AT]^C BsmBI 1679 GAGACG CGTCTC(1/5) BspDI 937 ATCGAT AT^CGAT BspHI 84 TCATGA T^CATGA BspMI 65 ACCTGC ACCTGC(4/8) BsrBI 609 CCGCTC CCGCTC(-3/-3) BsrDI 117 GCAATG GCAATG(2/0) BstBI 881 TTCGAA TT^CGAA BstUI 80 CGCG CG^CG BstXI 1028 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2770 GCTCAGC GC^T[ACGT]AGC ClaI 937 ATCGAT AT^CGAT DraI 336 TTTAAA TTT^AAA DraII 2873 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 1279 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 792 GACATTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 6 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 66 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2873 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 1800 ATGCAT ATGCA^T FauI 777 GCGGG CCCGC(4/6) FspI 932 TGCGCA TGC^GCA HindIII 524 AAGCTT A^AGCTT MspA1I 188 CAGCTG C[AC]G^C[GT]G MvnI 80 CGCG CG^CG NciI 1911 CCGGG CC^[GC]GG NsiI 1800 ATGCAT ATGCA^T NspV 881 TTCGAA TT^CGAA PpuMI 2873 GGGACCT [GA]G^G[AT]CC[CT] PvuII 188 CAGCTG CAG^CTG RcaI 84 TCATGA T^CATGA SacI 2317 GAGCTC GAGCT^C SfcI 740 CTACAG C^T[GA][CT]AG SfiI 2034 GGCCACGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 881 TTCGAA TT^CGAA XbaI 2859 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 1768 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1790 CAGTGGCTG BalI 1811 TGGCCA TGG^CCA 2033 TGGCCA BamHI 2074 GGATCC G^GATCC 2521 GGATCC BanI 1287 GGCACC G^G[CT][GA]CC 1690 GGTGCC BpmI 910 CTCCAG CTGGAG(16/14) 1615 CTGGAG BsgI 22 GTGCAG GTGCAG(16/14) 1382 GTGCAG BssSI 349 CACGAG CACGAG(-5/-1) 1783 CACGAG EaeI 1811 TGGCCA [CT]^GGCC[GA] 2033 TGGCCA HapII 1911 CCGG C^CGG 2072 CCGG HgaI 473 GACGC GACGC(5/10) 81 GCGTC HincII 499 GTCAAC GT[CT]^[GA]AC 951 GTCAAC HindII 499 GTCAAC GT[CT]^[GA]AC 951 GTCAAC HpaII 1911 CCGG C^CGG 2072 CCGG MfeI 227 CAATTG C^AATTG 1634 CAATTG MluNI 1811 TGGCCA TGG^CCA 2033 TGGCCA MscI 1811 TGGCCA TGG^CCA 2033 TGGCCA MslI 619 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1029 CATCCTGGTG MspI 1911 CCGG C^CGG 2072 CCGG MunI 227 CAATTG C^AATTG 1634 CAATTG NspI 1361 ACATGC [GA]CATG^[CT] 2330 GCATGT PflMI 1814 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2428 CCAAGGTTTGG SapI 1596 GAAGAGC GCTCTTC(1/4) 2319 GCTCTTC StuI 1859 AGGCCT AGG^CCT 2624 AGGCCT Van91I 1814 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2428 CCAAGGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BanII BbvI BfaI BglII BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI