################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NciI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 347 GTAGAC GT^[AC][GT]AC AcyI 883 GGCGCC G[GA]^CG[CT]C AfaI 495 GTAC GT^AC AocI 639 CCTAAGG CC^T[ACGT]AGG AvaII 652 GGACC G^G[AT]CC BanII 265 GGGCTC G[GA]GC[CT]^C BbeI 883 GGCGCC GGCGC^C BclI 564 TGATCA T^GATCA BpmI 832 CTCCAG CTGGAG(16/14) BsaHI 883 GGCGCC G[GA]^CG[CT]C BsaWI 37 ACCGGA [AT]^CCGG[AT] BsmAI 778 GAGAC GTCTC(1/5) BssKI 353 CCAGG ^CC[ACGT]GG BstBI 718 TTCGAA TT^CGAA BstNI 353 CCAGG CC^[AT]GG Bsu36I 639 CCTAAGG CC^T[ACGT]AGG Cac8I 634 GCTGGC GC[ACGT]^[ACGT]GC CfoI 884 GCGC GCG^C Cfr13I 652 GGACC G^G[ACGT]CC DdeI 640 CTAAG C^T[ACGT]AG DraII 651 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 75 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 243 CCATGG C^C[GA][CT]GG Eco81I 639 CCTAAGG CC^T[ACGT]AGG EcoO109I 651 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1006 GAATTC G^AATTC EcoRII 353 CCAGG ^CC[AT]GG FbaI 564 TGATCA T^GATCA HaeII 883 GGCGCC [GA]GCGC^[CT] HapII 38 CCGG C^CGG HhaI 884 GCGC GCG^C Hin1I 883 GGCGCC G[GA]^CG[CT]C HinP1I 884 GCGC G^CGC HpaII 38 CCGG C^CGG HphI 775 GGTGA GGTGA(8/7) KasI 883 GGCGCC G^GCGCC MaeII 88 ACGT A^CGT MaeIII 733 GTGAC ^GT[ACGT]AC MspI 38 CCGG C^CGG MvaI 353 CCAGG CC^[AT]GG NarI 883 GGCGCC GG^CGCC NcoI 243 CCATGG C^CATGG NdeI 944 CATATG CA^TATG NspV 718 TTCGAA TT^CGAA PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT] RsaI 495 GTAC GT^AC Sau96I 652 GGACC G^G[ACGT]CC ScrFI 353 CCAGG CC^[ACGT]GG SfoI 883 GGCGCC GGC^GCC SfuI 718 TTCGAA TT^CGAA SspI 211 AATATT AAT^ATT StuI 685 AGGCCT AGG^CCT TaqI 719 TCGA T^CGA Tsp45I 733 GTGAC ^GT[GC]AC TthHB8I 719 TCGA T^CGA XcmI 577 CCAATTCCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 7 GCGG AcsI 279 GAATTT [GA]^AATT[CT] 1006 GAATTC AlwI 890 GATCC GGATC(4/5) 590 GGATC ApoI 279 GAATTT [GA]^AATT[CT] 1006 GAATTC Asp700I 157 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 701 GAAAGTATTC AvrII 423 CCTAGG C^CTAGG 459 CCTAGG BbsI 721 GAAGAC GAAGAC(2/6) 951 GAAGAC BglII 288 AGATCT A^GATCT 1028 AGATCT BlnI 423 CCTAGG C^CTAGG 459 CCTAGG BpuAI 721 GAAGAC GAAGAC(2/6) 951 GAAGAC BsgI 673 GTGCAG GTGCAG(16/14) 760 GTGCAG BstXI 243 CCATGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 834 CCAGCGAATTGG BstYI 288 AGATCT [GA]^GATC[CT] 1028 AGATCT EarI 115 GAAGAG CTCTTC(1/4) 406 GAAGAG Ksp632I 115 GAAGAG CTCTTC(1/4) 406 GAAGAG MflI 288 AGATCT [GA]^GATC[CT] 1028 AGATCT NlaIII 77 CATG CATG^ 244 CATG SfaNI 367 GATGC GCATC(5/9) 920 GCATC XhoII 288 AGATCT [GA]^GATC[CT] 1028 AGATCT XmnI 157 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 701 GAAAGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AluI BanI BbvI BfaI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BstF5I DpnII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MboI MboII MnlI MseI MslI MwoI NdeII NlaIV PleI Sau3AI StyI TfiI Tru9I TseI Tsp509I TspRI