Restriction map for
pFN21AE6314
(Circular) (Six-base) MAPSORT of: pFN21AE6314.seq Check: 5466 from: 1 to: 5752
pFN21AE6314 5752 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2353 3095 1937
Size: 416 742 4594
Fragments arranged by size:
4594 742 416
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2139 2222 2516 3815 5199 5467 1632
Size: 507 83 294 1299 1384 268 1917
Fragments arranged by size:
1917 1384 1299 507 294 268 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2284 3011 3061 3287 3439 5548 1493
Size: 791 727 50 226 152 2109 1697
Fragments arranged by size:
2109 1697 791 727 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3077 1870
Size: 73 1134 4545
Fragments arranged by size:
4545 1134 73
AvrII C'CTAG_G
Cuts at: 2428 2464 2428
Size: 36 5716
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2888 2918 3073 4528
Size: 325 217 627 298 803 30 155 1455
Cuts at: 4528 618
Size: 1842
Fragments arranged by size:
1842 1455 803 627 325 298 217 155
30
BanII G_rGCy'C
Cuts at: 1391 2274 3071 1391
Size: 883 797 4072
Fragments arranged by size:
4072 883 797
BbeI G_GCGC'C
Cuts at: 2892 2892
Size: 5752
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2733 2963 928
Size: 971 834 230 3717
Fragments arranged by size:
3717 971 834 230
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 2569 1548
Size: 1021 4731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4363 136
Size: 107 122 71 631 605 337 2354 1525
Fragments arranged by size:
2354 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2293 3033 5747 2293
Size: 740 2714 2298
Fragments arranged by size:
2714 2298 740
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2281 2428 2460 3813 4969 1790
Size: 300 191 147 32 1353 1156 2573
Fragments arranged by size:
2573 1353 1156 300 191 147 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2822 4412 1239
Size: 1583 1590 2579
Fragments arranged by size:
2579 1590 1583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2889 3559 3941 4576
Size: 53 83 186 936 1356 670 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1356 936 670 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2042 4185 4583 4857 5004 5415 2042
Size: 2143 398 274 147 411 2379
Fragments arranged by size:
2379 2143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 1178
Size: 30 165 30 975 30 4522
Fragments arranged by size:
4522 975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2033 2036 2274 1186
Size: 844 3 3 238 4664
Fragments arranged by size:
4664 844 238 3 3
BseYI C'CCAG_C
Cuts at: 4955 5451 4955
Size: 496 5256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 2786 1163
Size: 573 963 87 4129
Fragments arranged by size:
4129 963 573 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2274 2281 2428 2460 3071 3813
Size: 399 300 184 7 147 32 611 742
Cuts at: 3813 3898 4969 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 399 300 184 147
85 32 7
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 2724 4556 2724
Size: 1832 3920
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2255 2846 2255
Size: 591 5161
BstYI r'GATC_y
Cuts at: 1972 2293 3033 3082 3449 3834 3851 5303 5389
Size: 321 740 49 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 740 385 367 346 321 86
49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2645 2645
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2248 513
Size: 552 939 244 4017
Fragments arranged by size:
4017 939 552 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2449 2484 529
Size: 35 1885 35 3797
Fragments arranged by size:
3797 1885 35 35
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2085 2085
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3199 4088 5717
Size: 54 1425 50 275 18 1369 889 1629
Cuts at: 5717 8
Size: 43
Fragments arranged by size:
1629 1425 1369 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2406 3682 1353
Size: 762 291 1276 3423
Fragments arranged by size:
3423 1276 762 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 4339 4713 4859 387
Size: 1064 138 438 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2222 2516 2822 3815 4412 5199
Size: 393 507 83 294 306 993 597 787
Cuts at: 5199 5467 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 597 507 393 306 294
268 83
EcoO109I rG'GnC_Cy
Cuts at: 2657 2657
Size: 5752
EcoRI G'AATT_C
Cuts at: 3011 3061 3439 3011
Size: 50 378 5324
Fragments arranged by size:
5324 378 50
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2892 4899 1678
Size: 1214 2007 2531
Fragments arranged by size:
2531 2007 1214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KasI G'GCGC_C
Cuts at: 2888 2888
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2760 2949 3710 4069 4228 518
Size: 648 1362 232 189 761 359 159 2042
Fragments arranged by size:
2042 1362 761 648 359 232 189 159
MspA1I CmG'CkG
Cuts at: 1606 3168 3845 4993 5238 1606
Size: 1562 677 1148 245 2120
Fragments arranged by size:
2120 1562 1148 677 245
NarI GG'CG_CC
Cuts at: 2889 2889
Size: 5752
NcoI C'CATG_G
Cuts at: 513 1065 2004 2248 513
Size: 552 939 244 4017
Fragments arranged by size:
4017 939 552 244
NdeI CA'TA_TG
Cuts at: 387 2950 387
Size: 2563 3189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2657 2657
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2890 2890
Size: 5752
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 2218 3676 5
Size: 47 2166 1458 2081
Fragments arranged by size:
2166 2081 1458 47
StuI AGG'CCT
Cuts at: 1517 2692 1517
Size: 1175 4577
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2428 2464 2537 2604 3191
Size: 552 939 244 180 36 73 67 587
Cuts at: 3191 513
Size: 3074
Fragments arranged by size:
3074 939 587 552 244 180 73 67
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2589 2589
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 2166 2710 3881 2166
Size: 544 1171 4037
Fragments arranged by size:
4037 1171 544
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI