################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbsI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsmI BspDI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MseI MunI NdeI NheI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1170 GACGTC GACGT^C AccIII 566 TCCGGA T^CCGGA Alw44I 1303 GTGCAC G^TGCAC AlwI 549 GATCC GGATC(4/5) ApaLI 1303 GTGCAC G^TGCAC Asp700I 18 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1388 GCAAGATTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1335 GTATCC GTATCC(6/5) BsaWI 566 TCCGGA [AT]^CCGG[AT] BseAI 566 TCCGGA T^CCGGA BsiEI 1383 CGGCCG CG[GA][CT]^CG BsiWI 147 CGTACG C^GTACG BsmBI 1168 GAGACG CGTCTC(1/5) Bsp1407I 632 TGTACA T^GTACA BspEI 566 TCCGGA T^CCGGA BsrGI 632 TGTACA T^GTACA BssHII 515 GCGCGC G^CGCGC BssSI 945 CACGAG CACGAG(-5/-1) BstF5I 882 GGATG GGATG(2/0) BstXI 215 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 548 AGATCC [GA]^GATC[CT] DdeI 968 CTAAG C^T[ACGT]AG EagI 1383 CGGCCG C^GGCCG EarI 994 CTCTTC CTCTTC(1/4) EclXI 1383 CGGCCG C^GGCCG Eco52I 1383 CGGCCG C^GGCCG FokI 882 GGATG GGATG(9/13) Ksp632I 994 CTCTTC CTCTTC(1/4) MflI 548 AGATCC [GA]^GATC[CT] MroI 566 TCCGGA T^CCGGA MslI 1059 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 258 GCCGGC GCC^GGC NcoI 1440 CCATGG C^CATGG NgoMIV 258 GCCGGC G^CCGGC NotI 1382 GCGGCCGC GC^GGCCGC NspI 824 GCATGC [GA]CATG^[CT] PaeR7I 640 CTCGAG C^TCGAG PflFI 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 720 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 643 GAGTC GAGTC(4/5) SacI 34 GAGCTC GAGCT^C SapI 993 GCTCTTC GCTCTTC(1/4) SphI 824 GCATGC GCATG^C SspBI 632 TGTACA T^GTACA StuI 170 AGGCCT AGG^CCT TfiI 1392 GATTC G^A[AT]TC Tsp45I 1645 GTGAC ^GT[GC]AC Tsp509I 10 AATT ^AATT Tth111I 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 720 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 640 CTCGAG C^TCGAG XhoII 548 AGATCC [GA]^GATC[CT] XmnI 18 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### ApaI 1179 GGGCCC GGGCC^C 1249 GGGCCC AspHI 34 GAGCTC G[AT]GC[AT]^C 1303 GTGCAC AvaI 640 CTCGAG C^[CT]CG[GA]G 814 CTCGGG BalI 1010 TGGCCA TGG^CCA 1433 TGGCCA BbeI 333 GGCGCC GGCGC^C 1291 GGCGCC BbrPI 244 CACGTG CAC^GTG 352 CACGTG BsiHKAI 34 GAGCTC G[AT]GC[AT]^C 1303 GTGCAC BsmAI 1168 GAGAC GTCTC(1/5) 1565 GAGAC BsoBI 640 CTCGAG C^[CT]CG[GA]G 814 CTCGGG BspHI 578 TCATGA T^CATGA 893 TCATGA BsrBI 1464 CCGCTC CCGCTC(-3/-3) 1513 CCGCTC DpnII 549 GATC ^GATC 972 GATC EcoT14I 131 CCAAGG C^C[AT][AT]GG 1440 CCATGG HgaI 360 GACGC GACGC(5/10) 1576 GACGC HinfI 643 GAGTC G^A[ACGT]TC 1392 GATTC HphI 1222 GGTGA GGTGA(8/7) 212 TCACC KasI 333 GGCGCC G^GCGCC 1291 GGCGCC KspI 288 CCGCGG CCGC^GG 387 CCGCGG MaeIII 1418 GTTAC ^GT[ACGT]AC 1645 GTGAC MboI 549 GATC ^GATC 972 GATC MluNI 1010 TGGCCA TGG^CCA 1433 TGGCCA MscI 1010 TGGCCA TGG^CCA 1433 TGGCCA NarI 333 GGCGCC GG^CGCC 1291 GGCGCC NdeII 549 GATC ^GATC 972 GATC PmaCI 244 CACGTG CAC^GTG 352 CACGTG PmlI 244 CACGTG CAC^GTG 352 CACGTG RcaI 578 TCATGA T^CATGA 893 TCATGA SacII 288 CCGCGG CCGC^GG 387 CCGCGG Sau3AI 549 GATC ^GATC 972 GATC ScaI 494 AGTACT AGT^ACT 1502 AGTACT SfoI 333 GGCGCC GGC^GCC 1291 GGCGCC SgrAI 257 CGCCGGCG C[GA]^CCGG[CT]G 1061 CGCCGGTG SmlI 640 CTCGAG C^T[CT][GA]AG 1308 CTCAAG StyI 131 CCAAGG C^C[AT][AT]GG 1440 CCATGG TaqI 26 TCGA T^CGA 641 TCGA TthHB8I 26 TCGA T^CGA 641 TCGA XcmI 836 CCAAGAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1262 CCACGCAGCACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DraIII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI TseI TspRI