(Linear) (Six-base) MAPSORT of: KIEE3926.seq Check: 2765 from: 1 to: 1653 KIEE3926.orf With 182 enzymes: SgfI * March 22, 2012 16:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 374 468 1653 Size: 374 94 1185 Fragments arranged by size: 1185 374 94 AatII G_ACGT'C Cuts at: 0 1174 1653 Size: 1174 479 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 703 1038 1653 Size: 226 477 335 615 Fragments arranged by size: 615 477 335 226 AleI CACnn'nnGTG Cuts at: 0 1063 1653 Size: 1063 590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1081 1653 Size: 1081 572 AlwNI CAG_nnn'CTG Cuts at: 0 726 807 1245 1568 1653 Size: 726 81 438 323 85 Fragments arranged by size: 726 438 323 85 81 ApaI G_GGCC'C Cuts at: 0 1183 1253 1653 Size: 1183 70 400 Fragments arranged by size: 1183 400 70 ApaLI G'TGCA_C Cuts at: 0 1303 1653 Size: 1303 350 AvaI C'yCGr_G Cuts at: 0 640 814 1653 Size: 640 174 839 Fragments arranged by size: 839 640 174 BanI G'GyrC_C Cuts at: 0 333 392 1065 1291 1653 Size: 333 59 673 226 362 Fragments arranged by size: 673 362 333 226 59 BanII G_rGCy'C Cuts at: 0 38 853 1183 1253 1653 Size: 38 815 330 70 400 Fragments arranged by size: 815 400 330 70 38 BbeI G_GCGC'C Cuts at: 0 337 1295 1653 Size: 337 958 358 Fragments arranged by size: 958 358 337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1377 1411 1653 Size: 1377 34 242 Fragments arranged by size: 1377 242 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1346 1653 Size: 1346 307 BglI GCCn_nnn'nGGC Cuts at: 0 290 1187 1248 1653 Size: 290 897 61 405 Fragments arranged by size: 897 405 290 61 Bme1580I G_kGCm'C Cuts at: 0 395 1070 1183 1213 1253 1307 1493 1653 Size: 395 675 113 30 40 54 186 160 Fragments arranged by size: 675 395 186 160 113 54 40 30 BmgBI CAC'GTC Cuts at: 0 417 543 1653 Size: 417 126 1110 Fragments arranged by size: 1110 417 126 BmrI ACTGGGnnnn_n' Cuts at: 0 1368 1533 1653 Size: 1368 165 120 Fragments arranged by size: 1368 165 120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 447 619 1653 Size: 214 233 172 1034 Fragments arranged by size: 1034 233 214 172 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1293 1653 Size: 1293 360 BsaAI yAC'GTr Cuts at: 0 246 354 1644 1653 Size: 246 108 1290 9 Fragments arranged by size: 1290 246 108 9 BsaHI Gr'CG_yC Cuts at: 0 334 1171 1292 1577 1653 Size: 334 837 121 285 76 Fragments arranged by size: 837 334 285 121 76 BsaWI w'CCGG_w Cuts at: 0 566 1653 Size: 566 1087 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 970 1000 1450 1480 1499 1529 1653 Size: 970 30 450 30 19 30 124 Fragments arranged by size: 970 450 124 30 30 30 19 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 46 616 679 1653 Size: 43 3 570 63 974 Fragments arranged by size: 974 570 63 43 3 BseYI C'CCAG_C Cuts at: 0 846 1355 1549 1583 1653 Size: 846 509 194 34 70 Fragments arranged by size: 846 509 194 70 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 97 226 253 376 700 793 927 Size: 40 57 129 27 123 324 93 134 Cuts at: 927 1653 Size: 726 Fragments arranged by size: 726 324 134 129 123 93 57 40 27 BsiEI CG_ry'CG Cuts at: 0 1386 1653 Size: 1386 267 BsiHKAI G_wGCw'C Cuts at: 0 38 1307 1653 Size: 38 1269 346 Fragments arranged by size: 1269 346 38 BsiWI C'GTAC_G Cuts at: 0 147 1653 Size: 147 1506 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1162 1653 Size: 1162 491 Bsp1286I G_dGCh'C Cuts at: 0 38 395 853 1070 1183 1213 1253 1307 Size: 38 357 458 217 113 30 40 54 Cuts at: 1307 1493 1653 Size: 186 160 Fragments arranged by size: 458 357 217 186 160 113 54 40 38 30 BspEI T'CCGG_A Cuts at: 0 566 1653 Size: 566 1087 BspHI T'CATG_A Cuts at: 0 578 893 1653 Size: 578 315 760 Fragments arranged by size: 760 578 315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 374 468 746 949 1653 Size: 374 94 278 203 704 Fragments arranged by size: 704 374 278 203 94 BsrBI CCG'CTC Cuts at: 0 1466 1515 1653 Size: 1466 49 138 Fragments arranged by size: 1466 138 49 BsrFI r'CCGG_y Cuts at: 0 258 593 1062 1653 Size: 258 335 469 591 Fragments arranged by size: 591 469 335 258 BsrGI T'GTAC_A Cuts at: 0 632 1653 Size: 632 1021 BssHII G'CGCG_C Cuts at: 0 515 1653 Size: 515 1138 BssSI C'ACGA_G Cuts at: 0 945 1653 Size: 945 708 BstAPI GCAn_nnn'nTGC Cuts at: 0 723 807 1365 1653 Size: 723 84 558 288 Fragments arranged by size: 723 558 288 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 222 1653 Size: 222 1431 BstYI r'GATC_y Cuts at: 0 548 1653 Size: 548 1105 BtgI C'CryG_G Cuts at: 0 288 387 1430 1440 1653 Size: 288 99 1043 10 213 Fragments arranged by size: 1043 288 213 99 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1313 1653 Size: 1313 340 BtsI GCAGTG_nn' Cuts at: 0 270 462 1653 Size: 270 192 1191 Fragments arranged by size: 1191 270 192 DraIII CAC_nnn'GTG Cuts at: 0 276 534 1275 1644 1653 Size: 276 258 741 369 9 Fragments arranged by size: 741 369 276 258 9 EaeI y'GGCC_r Cuts at: 0 482 975 1010 1191 1383 1433 1446 1470 Size: 482 493 35 181 192 50 13 24 Cuts at: 1470 1593 1653 Size: 123 60 Fragments arranged by size: 493 482 192 181 123 60 50 35 24 13 EagI C'GGCC_G Cuts at: 0 1383 1653 Size: 1383 270 EarI CTCTTCn'nnn_ Cuts at: 0 1000 1653 Size: 1000 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 424 1135 1338 1653 Size: 19 405 711 203 315 Fragments arranged by size: 711 405 315 203 19 EcoICRI GAG'CTC Cuts at: 0 36 1653 Size: 36 1617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 226 447 619 703 1038 1653 Size: 214 12 221 172 84 335 615 Fragments arranged by size: 615 335 221 214 172 84 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 403 661 763 1653 Size: 403 258 102 890 Fragments arranged by size: 890 403 258 102 EcoO109I rG'GnC_Cy Cuts at: 0 735 756 932 1048 1180 1249 1250 1653 Size: 735 21 176 116 132 69 1 403 Fragments arranged by size: 735 403 176 132 116 69 21 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 39 1653 Size: 39 1614 HaeII r_GCGC'y Cuts at: 0 268 337 657 808 1295 1653 Size: 268 69 320 151 487 358 Fragments arranged by size: 487 358 320 268 151 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1216 1248 1653 Size: 1159 32 25 32 405 Fragments arranged by size: 1159 405 32 32 25 KasI G'GCGC_C Cuts at: 0 333 1291 1653 Size: 333 958 362 Fragments arranged by size: 958 362 333 MscI TGG'CCA Cuts at: 0 1012 1435 1653 Size: 1012 423 218 Fragments arranged by size: 1012 423 218 MslI CAynn'nnrTG Cuts at: 0 1063 1653 Size: 1063 590 MspA1I CmG'CkG Cuts at: 0 81 140 290 300 389 512 630 723 Size: 81 59 150 10 89 123 118 93 Cuts at: 723 861 1029 1220 1653 Size: 138 168 191 433 Fragments arranged by size: 433 191 168 150 138 123 118 93 89 81 59 10 NaeI GCC'GGC Cuts at: 0 260 1653 Size: 260 1393 NarI GG'CG_CC Cuts at: 0 334 1292 1653 Size: 334 958 361 Fragments arranged by size: 958 361 334 NcoI C'CATG_G Cuts at: 0 1440 1653 Size: 1440 213 NgoMIV G'CCGG_C Cuts at: 0 258 1653 Size: 258 1395 NotI GC'GGCC_GC Cuts at: 0 1383 1653 Size: 1383 270 NspI r_CATG'y Cuts at: 0 828 1653 Size: 828 825 PasI CC'CwG_GG Cuts at: 0 1245 1653 Size: 1245 408 PflMI CCAn_nnn'nTGG Cuts at: 0 726 1653 Size: 726 927 PmlI CAC'GTG Cuts at: 0 246 354 1653 Size: 246 108 1299 Fragments arranged by size: 1299 246 108 PpuMI rG'GwC_Cy Cuts at: 0 735 756 932 1653 Size: 735 21 176 721 Fragments arranged by size: 735 721 176 21 PspOMI G'GGCC_C Cuts at: 0 1179 1249 1653 Size: 1179 70 404 Fragments arranged by size: 1179 404 70 PspXI vC'TCGA_Gb Cuts at: 0 640 1653 Size: 640 1013 PstI C_TGCA'G Cuts at: 0 371 704 787 860 1079 1258 1653 Size: 371 333 83 73 219 179 395 Fragments arranged by size: 395 371 333 219 179 83 73 PvuII CAG'CTG Cuts at: 0 81 630 723 861 1029 1653 Size: 81 549 93 138 168 624 Fragments arranged by size: 624 549 168 138 93 81 SacI G_AGCT'C Cuts at: 0 38 1653 Size: 38 1615 SacII CC_GC'GG Cuts at: 0 291 390 1653 Size: 291 99 1263 Fragments arranged by size: 1263 291 99 SapI GCTCTTCn'nnn_ Cuts at: 0 1000 1653 Size: 1000 653 ScaI AGT'ACT Cuts at: 0 496 1504 1653 Size: 496 1008 149 Fragments arranged by size: 1008 496 149 SfcI C'TryA_G Cuts at: 0 367 507 700 783 856 1075 1254 1608 Size: 367 140 193 83 73 219 179 354 Cuts at: 1608 1653 Size: 45 Fragments arranged by size: 367 354 219 193 179 140 83 73 45 SfoI GGC'GCC Cuts at: 0 335 1293 1653 Size: 335 958 360 Fragments arranged by size: 958 360 335 SgrAI Cr'CCGG_yG Cuts at: 0 258 1062 1653 Size: 258 804 591 Fragments arranged by size: 804 591 258 SmlI C'TyrA_G Cuts at: 0 640 1308 1653 Size: 640 668 345 Fragments arranged by size: 668 640 345 SphI G_CATG'C Cuts at: 0 828 1653 Size: 828 825 StuI AGG'CCT Cuts at: 0 172 1653 Size: 172 1481 StyI C'CwwG_G Cuts at: 0 131 1440 1653 Size: 131 1309 213 Fragments arranged by size: 1309 213 131 TatI w'GTAC_w Cuts at: 0 494 632 1502 1653 Size: 494 138 870 151 Fragments arranged by size: 870 494 151 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 518 550 970 1002 1499 1517 1531 1549 Size: 518 32 420 32 497 18 14 18 Cuts at: 1549 1653 Size: 104 Fragments arranged by size: 518 497 420 104 32 32 18 18 14 Tth111I GACn'n_nGTC Cuts at: 0 1173 1653 Size: 1173 480 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 843 1269 1653 Size: 843 426 384 Fragments arranged by size: 843 426 384 XhoI C'TCGA_G Cuts at: 0 640 1653 Size: 640 1013 XmnI GAAnn'nnTTC Cuts at: 0 22 1653 Size: 22 1631 ZraI GAC'GTC Cuts at: 0 1172 1653 Size: 1172 481 Enzymes that do cut: AarI AatII AcuI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BciVI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI ScaI SfcI SfoI SgrAI SmlI SphI StuI StyI TatI TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI BsmI BsrDI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI TaqII XbaI XmaI