(Circular) (Six-base) MAPSORT of: pFN21AE4422.seq Check: 6375 from: 1 to: 6016 pFN21AE4422 6016 bp With 182 enzymes: SgfI * March 28, 2012 13:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2156 2156 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3359 1937 Size: 217 1205 4594 Fragments arranged by size: 4594 1205 217 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 4079 5463 5731 1632 Size: 1046 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2774 3123 4915 5892 1203 Size: 1571 349 1792 977 1327 Fragments arranged by size: 1792 1571 1327 977 349 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2120 3251 1184 Size: 936 1131 3949 Fragments arranged by size: 3949 1131 936 AlwNI CAG_nnn'CTG Cuts at: 3759 5331 3759 Size: 1572 4444 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2213 2272 2492 2786 2820 3325 3551 3703 Size: 720 59 220 294 34 505 226 152 Cuts at: 3703 5812 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 720 505 294 226 220 152 59 34 AseI AT'TA_AT Cuts at: 160 1032 2448 2649 4570 160 Size: 872 1416 201 1921 1606 Fragments arranged by size: 1921 1606 1416 872 201 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 3341 1870 Size: 73 1398 4545 Fragments arranged by size: 4545 1398 73 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2635 3337 4792 618 Size: 325 217 627 848 702 1455 1842 Fragments arranged by size: 1842 1455 848 702 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 3335 1391 Size: 693 1251 4072 Fragments arranged by size: 4072 1251 693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 928 Size: 971 573 4472 Fragments arranged by size: 4472 971 573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4207 4241 1903 Size: 34 50 34 2186 34 3678 Fragments arranged by size: 3678 2186 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3321 3908 5118 3321 Size: 587 1210 4219 Fragments arranged by size: 4219 1210 587 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2976 4077 5233 1790 Size: 1186 1101 1156 2573 Fragments arranged by size: 2573 1186 1156 1101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 3747 4705 Size: 416 53 83 399 24 1778 552 958 Cuts at: 4705 442 Size: 1753 Fragments arranged by size: 1778 1753 958 552 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4676 1239 Size: 3437 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4141 5006 5304 4141 Size: 865 298 4853 Fragments arranged by size: 4853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2028 4679 882 Size: 617 529 2651 2219 Fragments arranged by size: 2651 2219 617 529 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5791 Fragments arranged by size: 5791 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6016 BseYI C'CCAG_C Cuts at: 2029 5219 5715 2029 Size: 3190 496 2330 Fragments arranged by size: 3190 2330 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2706 1163 Size: 573 970 4473 Fragments arranged by size: 4473 970 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2146 3466 4227 5255 1540 Size: 275 186 145 1320 761 1028 2301 Fragments arranged by size: 2301 1320 1028 761 275 186 145 BsiHKAI G_wGCw'C Cuts at: 4077 4162 5233 4077 Size: 85 1071 4860 Fragments arranged by size: 4860 1071 85 BsmI GAATG_Cn' Cuts at: 3527 3620 3527 Size: 93 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2976 3335 4077 4162 5233 1391 Size: 399 294 892 359 742 85 1071 2174 Fragments arranged by size: 2174 1071 892 742 399 359 294 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2156 3371 844 Size: 1312 1215 3489 Fragments arranged by size: 3489 1312 1215 BsrBI CCG'CTC Cuts at: 1394 1823 3903 1394 Size: 429 2080 3507 Fragments arranged by size: 3507 2080 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 2115 96 Size: 2019 3997 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 3262 794 Size: 2468 3548 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 2282 1184 Size: 1098 4918 BstYI r'GATC_y Cuts at: 1972 3346 3713 4098 4115 5567 5653 5665 6011 Size: 1374 367 385 17 1452 86 12 346 Cuts at: 6011 1972 Size: 1977 Fragments arranged by size: 1977 1452 1374 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2155 2155 Size: 6016 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5464 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2568 2988 3532 4326 4346 2568 Size: 420 544 794 20 4238 Fragments arranged by size: 4238 794 544 420 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DraIII CAC_nnn'GTG Cuts at: 3181 3181 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2529 3463 4352 Size: 54 1425 50 275 18 699 934 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 934 889 699 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3946 1353 Size: 2593 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2678 4079 4676 5463 5731 1239 Size: 393 1046 1401 597 787 268 1524 Fragments arranged by size: 1524 1401 1046 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2407 2660 2407 Size: 253 5763 EcoO109I rG'GnC_Cy Cuts at: 2352 2352 Size: 6016 EcoRI G'AATT_C Cuts at: 2492 3325 3703 2492 Size: 833 378 4805 Fragments arranged by size: 4805 833 378 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2590 5163 1678 Size: 912 2573 2531 Fragments arranged by size: 2573 2531 912 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2734 2766 4657 4689 4731 Size: 32 135 32 1162 32 1891 32 42 Cuts at: 4731 4763 1373 Size: 32 2626 Fragments arranged by size: 2626 1891 1162 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3360 3606 669 Size: 988 281 1221 201 246 3079 Fragments arranged by size: 3079 1221 988 281 246 201 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 2009 2738 3615 2009 Size: 729 877 4410 Fragments arranged by size: 4410 877 729 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3097 5130 5314 1305 Size: 1792 2033 184 2007 Fragments arranged by size: 2033 2007 1792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 10 Size: 54 1425 343 699 3495 Fragments arranged by size: 3495 1425 699 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3087 3233 3974 4333 4492 518 Size: 648 1921 146 741 359 159 2042 Fragments arranged by size: 2042 1921 741 648 359 159 146 MspA1I CmG'CkG Cuts at: 1606 2363 3432 4109 5257 5502 1606 Size: 757 1069 677 1148 245 2120 Fragments arranged by size: 2120 1148 1069 757 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 2778 3058 3127 3238 3374 4919 1132 Size: 97 1549 280 69 111 136 1545 2229 Fragments arranged by size: 2229 1549 1545 280 136 111 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 2774 3123 4915 2774 Size: 349 1792 3875 Fragments arranged by size: 3875 1792 349 PflMI CCAn_nnn'nTGG Cuts at: 1108 2234 1108 Size: 1126 4890 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2352 2352 Size: 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PstI C_TGCA'G Cuts at: 830 2464 3368 830 Size: 1634 904 3478 Fragments arranged by size: 3478 1634 904 PvuI CG_AT'CG Cuts at: 2001 2146 2001 Size: 145 5871 PvuII CAG'CTG Cuts at: 2363 2363 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SanDI GG'GwC_CC Cuts at: 2352 2352 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2460 3297 3364 4499 5180 5371 826 Size: 219 1415 837 67 1135 681 191 1471 Fragments arranged by size: 1471 1415 1135 837 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4120 5021 5283 820 Size: 197 926 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 2201 3940 5 Size: 47 2149 1739 2081 Fragments arranged by size: 2149 2081 1739 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2130 3108 3455 513 Size: 552 1065 978 347 3074 Fragments arranged by size: 3074 1065 978 552 347 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2307 4043 4060 4213 4398 4843 325 Size: 1255 727 1736 17 153 185 445 1498 Fragments arranged by size: 1736 1498 1255 727 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 4262 5974 Size: 274 80 33 51 1443 138 2147 1712 Cuts at: 5974 96 Size: 138 Fragments arranged by size: 2147 1712 1443 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 5976 6008 3054 Size: 32 2890 32 3062 Fragments arranged by size: 3062 2890 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2058 1316 Size: 742 5274 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3182 3182 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 2868 4145 2868 Size: 1277 4739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI