(Circular) (Six-base) MAPSORT of: pFN21AE5301.seq Check: 666 from: 1 to: 5485 pFN21AE5301 5485 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2318 2318 Size: 5485 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3548 4932 5200 1632 Size: 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 3228 4800 3228 Size: 1572 3913 ApaI G_GGCC'C Cuts at: 2599 2599 Size: 5485 ApaLI G'TGCA_C Cuts at: 2263 3542 4698 2263 Size: 1279 1156 3050 Fragments arranged by size: 3050 1279 1156 ApoI r'AATT_y Cuts at: 1493 2357 2794 3020 3172 5281 1493 Size: 864 437 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 437 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2810 1870 Size: 73 159 708 4545 Fragments arranged by size: 4545 708 159 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2177 2469 2772 2806 Size: 325 217 627 363 27 292 303 34 Cuts at: 2806 4261 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 363 325 303 292 217 34 27 BanII G_rGCy'C Cuts at: 1391 2199 2599 2804 1391 Size: 808 400 205 4072 Fragments arranged by size: 4072 808 400 205 BbeI G_GCGC'C Cuts at: 2154 2154 Size: 5485 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2547 2740 Size: 107 122 71 631 605 487 388 193 Cuts at: 2740 4096 136 Size: 1356 1525 Fragments arranged by size: 1525 1356 631 605 487 388 193 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2180 2267 2295 2599 2775 3546 4702 1790 Size: 390 87 28 304 176 771 1156 2573 Fragments arranged by size: 2573 1156 771 390 304 176 87 28 BmgBI CAC'GTC Cuts at: 1318 2290 1318 Size: 972 4513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4174 442 Size: 416 53 83 399 24 1799 958 1753 Fragments arranged by size: 1799 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2591 4145 1239 Size: 1352 1554 2579 Fragments arranged by size: 2579 1554 1352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3610 4475 4773 3610 Size: 865 298 4322 Fragments arranged by size: 4322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2099 2151 3292 3674 Size: 53 83 186 936 566 52 1141 382 Cuts at: 3674 4309 275 Size: 635 1451 Fragments arranged by size: 1451 1141 936 635 566 382 186 83 53 52 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 1178 Size: 30 165 30 1236 30 3994 Fragments arranged by size: 3994 1236 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 1186 Size: 862 4623 BseYI C'CCAG_C Cuts at: 2443 4688 5184 2443 Size: 2245 496 2744 Fragments arranged by size: 2744 2245 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 2523 1163 Size: 573 593 194 4125 Fragments arranged by size: 4125 593 573 194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2199 2267 2493 3546 3631 4702 2199 Size: 68 226 1053 85 1071 2982 Fragments arranged by size: 2982 1071 1053 226 85 68 BsmI GAATG_Cn' Cuts at: 2383 2584 2996 3089 2383 Size: 201 412 93 4779 Fragments arranged by size: 4779 412 201 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2199 2267 2295 2493 2599 2775 Size: 399 390 19 68 28 198 106 176 Cuts at: 2775 2804 3546 3631 4702 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 198 176 106 85 68 29 28 19 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2318 2840 844 Size: 1232 242 522 3489 Fragments arranged by size: 3489 1232 522 242 BsrBI CCG'CTC Cuts at: 1394 1823 3372 1394 Size: 429 1549 3507 Fragments arranged by size: 3507 1549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3980 4154 65 Size: 1183 2732 174 1396 Fragments arranged by size: 2732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2061 2684 2708 4129 4316 1700 Size: 110 230 21 623 24 1421 187 2869 Fragments arranged by size: 2869 1421 623 230 187 110 24 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2055 2145 2172 2295 2319 2367 513 Size: 552 990 90 27 123 24 48 3631 Fragments arranged by size: 3631 990 552 123 90 48 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 2445 2729 3001 3795 3815 2445 Size: 284 272 794 20 4115 Fragments arranged by size: 4115 794 284 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2356 2789 2356 Size: 433 5052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2932 3821 Size: 54 1425 50 275 18 309 793 889 Cuts at: 3821 5450 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 793 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3415 1353 Size: 2062 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2300 4072 4446 4592 387 Size: 1064 138 711 1772 374 146 1280 Fragments arranged by size: 1772 1280 1064 711 374 146 138 EcoICRI GAG'CTC Cuts at: 2197 2197 Size: 5485 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2591 3548 4145 4932 5200 1239 Size: 393 959 957 597 787 268 1524 Fragments arranged by size: 1524 959 957 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2030 Size: 5485 EcoO109I rG'GnC_Cy Cuts at: 2595 2596 2751 2595 Size: 1 155 5329 Fragments arranged by size: 5329 155 1 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FseI GG_CCGG'CC Cuts at: 2688 2688 Size: 5485 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 2132 2154 2226 4632 1678 Size: 454 22 72 2406 2531 Fragments arranged by size: 2531 2406 454 72 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373 Size: 32 135 32 2554 32 42 32 2626 Fragments arranged by size: 2626 2554 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KasI G'GCGC_C Cuts at: 2150 2150 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 2503 4599 4783 1305 Size: 735 463 2096 184 2007 Fragments arranged by size: 2096 2007 735 463 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3663 Fragments arranged by size: 3663 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3443 3802 3961 518 Size: 648 1320 957 359 159 2042 Fragments arranged by size: 2042 1320 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2321 2426 2901 3578 4726 4971 1606 Size: 451 264 105 475 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 475 451 264 245 105 NaeI GCC'GGC Cuts at: 2063 2686 2710 2063 Size: 623 24 4838 Fragments arranged by size: 4838 623 24 NarI GG'CG_CC Cuts at: 2151 2151 Size: 5485 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NgoMIV G'CCGG_C Cuts at: 2061 2684 2708 2061 Size: 623 24 4838 Fragments arranged by size: 4838 623 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2843 4388 1132 Size: 97 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 97 PasI CC'CwG_GG Cuts at: 1348 2665 2745 1348 Size: 1317 80 4088 Fragments arranged by size: 4088 1317 80 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4583 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2751 2751 Size: 5485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PspOMI G'GGCC_C Cuts at: 2595 2595 Size: 5485 PstI C_TGCA'G Cuts at: 830 2607 2837 830 Size: 1777 230 3478 Fragments arranged by size: 3478 1777 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 PvuII CAG'CTG Cuts at: 2426 2426 Size: 5485 SacI G_AGCT'C Cuts at: 2199 2199 Size: 5485 SacII CC_GC'GG Cuts at: 2058 2322 2058 Size: 264 5221 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SanDI GG'GwC_CC Cuts at: 2751 2751 Size: 5485 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 2654 3733 5445 2654 Size: 1079 1712 2694 Fragments arranged by size: 2694 1712 1079 SfcI C'TryA_G Cuts at: 826 1045 2603 2833 3968 4649 4840 826 Size: 219 1558 230 1135 681 191 1471 Fragments arranged by size: 1558 1471 1135 681 230 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2547 2547 Size: 5485 SfoI GGC'GCC Cuts at: 2152 2152 Size: 5485 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3589 4490 4752 820 Size: 197 926 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 2550 1517 Size: 1033 4452 StyI C'CwwG_G Cuts at: 513 1065 2463 2924 513 Size: 552 1398 461 3074 Fragments arranged by size: 3074 1398 552 461 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2179 3512 3529 3682 3867 4312 325 Size: 1255 599 1333 17 153 185 445 1498 Fragments arranged by size: 1498 1333 1255 599 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2652 3731 5443 Size: 274 80 33 51 1443 675 1079 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1443 1079 675 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SrfI SwaI XcmI