(Linear) (Six-base) MAPSORT of: KIEE5301.seq Check: 9490 from: 1 to: 780 KIEE5301.orf With 182 enzymes: SgfI * December 5, 2014 13:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 313 780 Size: 313 467 ApaI G_GGCC'C Cuts at: 0 594 780 Size: 594 186 ApaLI G'TGCA_C Cuts at: 0 258 780 Size: 258 522 ApoI r'AATT_y Cuts at: 0 352 780 Size: 352 428 AvaI C'yCGr_G Cuts at: 0 97 780 Size: 97 683 BanI G'GyrC_C Cuts at: 0 145 172 464 767 780 Size: 145 27 292 303 13 Fragments arranged by size: 303 292 145 27 13 BanII G_rGCy'C Cuts at: 0 194 594 780 Size: 194 400 186 Fragments arranged by size: 400 194 186 BbeI G_GCGC'C Cuts at: 0 149 780 Size: 149 631 BglI GCCn_nnn'nGGC Cuts at: 0 154 542 735 780 Size: 154 388 193 45 Fragments arranged by size: 388 193 154 45 Bme1580I G_kGCm'C Cuts at: 0 175 262 290 594 770 780 Size: 175 87 28 304 176 10 Fragments arranged by size: 304 176 175 87 28 10 BmgBI CAC'GTC Cuts at: 0 285 780 Size: 285 495 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 780 Size: 586 194 BsaHI Gr'CG_yC Cuts at: 0 94 146 780 Size: 94 52 634 Fragments arranged by size: 634 94 52 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 634 664 780 Size: 634 30 116 Fragments arranged by size: 634 116 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 43 780 Size: 43 737 BseYI C'CCAG_C Cuts at: 0 438 780 Size: 438 342 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 518 780 Size: 324 194 262 Fragments arranged by size: 324 262 194 BsiHKAI G_wGCw'C Cuts at: 0 194 262 488 780 Size: 194 68 226 292 Fragments arranged by size: 292 226 194 68 BsmI GAATG_Cn' Cuts at: 0 378 579 780 Size: 378 201 201 Fragments arranged by size: 378 201 201 Bsp1286I G_dGCh'C Cuts at: 0 175 194 262 290 488 594 770 780 Size: 175 19 68 28 198 106 176 10 Fragments arranged by size: 198 176 175 106 68 28 19 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 71 313 780 Size: 71 242 467 Fragments arranged by size: 467 242 71 BsrFI r'CCGG_y Cuts at: 0 35 56 679 703 780 Size: 35 21 623 24 77 Fragments arranged by size: 623 77 35 24 21 BtgI C'CryG_G Cuts at: 0 50 140 167 290 314 362 780 Size: 50 90 27 123 24 48 418 Fragments arranged by size: 418 123 90 50 48 27 24 BtsI GCAGTG_nn' Cuts at: 0 440 724 780 Size: 440 284 56 Fragments arranged by size: 440 284 56 DraI TTT'AAA Cuts at: 0 351 780 Size: 351 429 EaeI y'GGCC_r Cuts at: 0 134 780 Size: 134 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 295 780 Size: 295 485 EcoICRI GAG'CTC Cuts at: 0 192 780 Size: 192 588 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 780 Size: 586 194 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 780 Size: 25 755 EcoO109I rG'GnC_Cy Cuts at: 0 590 591 746 780 Size: 590 1 155 34 Fragments arranged by size: 590 155 34 1 FseI GG_CCGG'CC Cuts at: 0 683 780 Size: 683 97 HaeII r_GCGC'y Cuts at: 0 127 149 221 780 Size: 127 22 72 559 Fragments arranged by size: 559 127 72 22 KasI G'GCGC_C Cuts at: 0 145 780 Size: 145 635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 35 498 780 Size: 35 463 282 Fragments arranged by size: 463 282 35 MslI CAynn'nnrTG Cuts at: 0 481 780 Size: 481 299 MspA1I CmG'CkG Cuts at: 0 52 316 421 780 Size: 52 264 105 359 Fragments arranged by size: 359 264 105 52 NaeI GCC'GGC Cuts at: 0 58 681 705 780 Size: 58 623 24 75 Fragments arranged by size: 623 75 58 24 NarI GG'CG_CC Cuts at: 0 146 780 Size: 146 634 NgoMIV G'CCGG_C Cuts at: 0 56 679 703 780 Size: 56 623 24 77 Fragments arranged by size: 623 77 56 24 PasI CC'CwG_GG Cuts at: 0 660 740 780 Size: 660 80 40 Fragments arranged by size: 660 80 40 PpuMI rG'GwC_Cy Cuts at: 0 746 780 Size: 746 34 PspOMI G'GGCC_C Cuts at: 0 590 780 Size: 590 190 PstI C_TGCA'G Cuts at: 0 602 780 Size: 602 178 PvuII CAG'CTG Cuts at: 0 421 780 Size: 421 359 SacI G_AGCT'C Cuts at: 0 194 780 Size: 194 586 SacII CC_GC'GG Cuts at: 0 53 317 780 Size: 53 264 463 Fragments arranged by size: 463 264 53 SanDI GG'GwC_CC Cuts at: 0 746 780 Size: 746 34 ScaI AGT'ACT Cuts at: 0 649 780 Size: 649 131 SfcI C'TryA_G Cuts at: 0 598 780 Size: 598 182 SfiI GGCCn_nnn'nGGCC Cuts at: 0 542 780 Size: 542 238 SfoI GGC'GCC Cuts at: 0 147 780 Size: 147 633 StuI AGG'CCT Cuts at: 0 545 780 Size: 545 235 StyI C'CwwG_G Cuts at: 0 458 780 Size: 458 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 174 780 Size: 174 606 TatI w'GTAC_w Cuts at: 0 647 780 Size: 647 133 Enzymes that do cut: AarI ApaI ApaLI ApoI AvaI BanI BanII BbeI BglI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BtgI BtsI DraI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI PpuMI PspOMI PstI PvuII SacI SacII SanDI ScaI SfcI SfiI SfoI StuI StyI TaqII TatI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI