################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BsiWI BsmBI BsmI BspHI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DraI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MamI MfeI MluI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tru9I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 599 TCCGGA T^CCGGA AlwNI 1198 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1179 CCTGAGG CC^T[ACGT]AGG Aor51HI 1460 AGCGCT AGC^GCT ApaI 1154 GGGCCC GGGCC^C BalI 779 TGGCCA TGG^CCA BamHI 93 GGATCC G^GATCC BbeI 36 GGCGCC GGCGC^C BbsI 1171 GAAGAC GAAGAC(2/6) BlpI 1951 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1951 GCTGAGC GC^T[ACGT]AGC BpuAI 1171 GAAGAC GAAGAC(2/6) BsaAI 1792 TACGTG [CT]AC^GT[GA] BsaI 1567 GAGACC GGTCTC(1/5) BsaWI 599 TCCGGA [AT]^CCGG[AT] BseAI 599 TCCGGA T^CCGGA BspDI 1855 ATCGAT AT^CGAT BspEI 599 TCCGGA T^CCGGA BspLU11I 701 ACATGT A^CATGT BsrDI 902 GCAATG GCAATG(2/0) BssSI 1142 CACGAG CACGAG(-5/-1) BstAPI 462 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1179 CCTGAGG CC^T[ACGT]AGG CelII 1951 GCTGAGC GC^T[ACGT]AGC ClaI 1855 ATCGAT AT^CGAT DraIII 175 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2056 GACGACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1460 AGCGCT AGC^GCT Eco81I 1179 CCTGAGG CC^T[ACGT]AGG HincII 2047 GTCAAC GT[CT]^[GA]AC HindII 2047 GTCAAC GT[CT]^[GA]AC KasI 36 GGCGCC G^GCGCC MluNI 779 TGGCCA TGG^CCA MroI 599 TCCGGA T^CCGGA MscI 779 TGGCCA TGG^CCA NarI 36 GGCGCC GG^CGCC NruI 342 TCGCGA TCG^CGA PpuMI 294 GGGACCT [GA]G^G[AT]CC[CT] SacI 1954 GAGCTC GAGCT^C SfoI 36 GGCGCC GGC^GCC SmaI 1253 CCCGGG CCC^GGG TfiI 1801 GAATC G^A[AT]TC Tsp509I 929 AATT ^AATT XmaCI 1253 CCCGGG C^CCGGG XmaI 1253 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 701 ACATGT A^C[GA][CT]GT 1494 ACGTGT Asp700I 687 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2092 GAAGATGTTC AspHI 1435 GAGCAC G[AT]GC[AT]^C 1954 GAGCTC AvaI 229 CCCGAG C^[CT]CG[GA]G 1253 CCCGGG BbvCI 1641 CCTCAGC CCTCAGC(-5/-2) 1087 GCTGAGG BsiEI 30 CGGCCG CG[GA][CT]^CG 967 CGGCCG BsiHKAI 1435 GAGCAC G[AT]GC[AT]^C 1954 GAGCTC BsmAI 721 GAGAC GTCTC(1/5) 1567 GAGAC BsoBI 229 CCCGAG C^[CT]CG[GA]G 1253 CCCGGG Bsp1407I 182 TGTACA T^GTACA 419 TGTACA BsrGI 182 TGTACA T^GTACA 419 TGTACA DraII 294 GGGACCT [GA]G^G[ACGT]CC[CT] 1154 GGGCCCT EagI 30 CGGCCG C^GGCCG 967 CGGCCG EclXI 30 CGGCCG C^GGCCG 967 CGGCCG Eco52I 30 CGGCCG C^GGCCG 967 CGGCCG EcoNI 1215 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2061 CCTCGTCAAGG EcoO109I 294 GGGACCT [GA]G^G[ACGT]CC[CT] 1154 GGGCCCT HgaI 4 GCGTC GACGC(5/10) 1163 GCGTC KspI 27 CCGCGG CCGC^GG 76 CCGCGG NcoI 259 CCATGG C^CATGG 495 CCATGG PleI 349 GACTC GAGTC(4/5) 723 GACTC SacII 27 CCGCGG CCGC^GG 76 CCGCGG SapI 2211 GAAGAGC GCTCTTC(1/4) 690 GCTCTTC SspBI 182 TGTACA T^GTACA 419 TGTACA XcmI 800 CCATCGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2108 CCACCAGCCAGGTGG XmnI 687 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2092 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I XhoII