(Circular) (Six-base) MAPSORT of: pFN21AE6708.seq Check: 2368 from: 1 to: 6922 pFN21AE6708 6922 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3228 3370 3228 Size: 142 6780 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4265 1937 Size: 2328 4594 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 2774 3218 3740 4985 6369 6637 1632 Size: 875 267 444 522 1245 1384 268 1917 Fragments arranged by size: 1917 1384 1245 875 522 444 268 267 AfeI AGC'GCT Cuts at: 3467 3467 Size: 6922 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 2706 3499 5821 6798 1203 Size: 1503 793 2322 977 1327 Fragments arranged by size: 2322 1503 1327 977 793 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 5651 5651 Size: 6922 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6922 AlwNI CAG_nnn'CTG Cuts at: 3208 4665 6237 3208 Size: 1457 1572 3893 Fragments arranged by size: 3893 1572 1457 ApaI G_GGCC'C Cuts at: 3163 3163 Size: 6922 ApaLI G'TGCA_C Cuts at: 4979 6135 4979 Size: 1156 5766 ApoI r'AATT_y Cuts at: 1493 4231 4457 4609 6718 1493 Size: 2738 226 152 2109 1697 Fragments arranged by size: 2738 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5476 160 Size: 872 4444 1606 Fragments arranged by size: 4444 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 2234 3258 4247 1870 Size: 73 291 1024 989 4545 Fragments arranged by size: 4545 1024 989 291 73 BamHI G'GATC_C Cuts at: 2098 4252 4619 2098 Size: 2154 367 4401 Fragments arranged by size: 4401 2154 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2632 2656 3020 4243 Size: 325 217 627 254 591 24 364 1223 Cuts at: 4243 5698 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1223 627 591 364 325 254 217 24 BanII G_rGCy'C Cuts at: 1391 2227 3163 3495 3963 4241 1391 Size: 836 936 332 468 278 4072 Fragments arranged by size: 4072 936 836 468 332 278 BbeI G_GCGC'C Cuts at: 2045 2045 Size: 6922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3183 928 Size: 971 1284 4667 Fragments arranged by size: 4667 1284 971 BbvCI CC'TCA_GC Cuts at: 3093 3647 3093 Size: 554 6368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5113 5147 1903 Size: 34 3176 34 3678 Fragments arranged by size: 3678 3176 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4814 6024 4814 Size: 1210 5712 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5533 136 Size: 107 122 71 631 605 3861 1525 Fragments arranged by size: 3861 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6917 6917 Size: 6922 BlpI GC'TnA_GC Cuts at: 3957 4339 3957 Size: 382 6540 Bme1580I G_kGCm'C Cuts at: 1790 3148 3163 4983 6139 1790 Size: 1358 15 1820 1156 2573 Fragments arranged by size: 2573 1820 1358 1156 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6922 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4653 5611 442 Size: 416 53 83 399 24 3236 958 1753 Fragments arranged by size: 3236 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 2426 2780 2798 3023 3273 3422 3620 Size: 943 244 354 18 225 250 149 198 Cuts at: 3620 3731 3902 5582 1239 Size: 111 171 1680 2579 Fragments arranged by size: 2579 1680 943 354 250 244 225 198 171 149 111 18 Bpu10I CC'TnA_GC Cuts at: 3093 3647 3093 Size: 554 6368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 2723 3287 5047 5912 6210 2360 Size: 363 564 1760 865 298 3072 Fragments arranged by size: 3072 1760 865 564 363 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3566 5585 882 Size: 617 2067 2019 2219 Fragments arranged by size: 2219 2067 2019 617 BsaAI yAC'GTr Cuts at: 493 1204 3799 4713 493 Size: 711 2595 914 2702 Fragments arranged by size: 2702 2595 914 711 BsaBI GATnn'nnATC Cuts at: 6916 6916 Size: 6922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2042 3168 4729 Size: 53 83 186 936 476 33 1126 1561 Cuts at: 4729 5111 5746 275 Size: 382 635 1451 Fragments arranged by size: 1561 1451 1126 936 635 476 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 2604 5355 5753 6027 6174 6585 2604 Size: 2751 398 274 147 411 2941 Fragments arranged by size: 2941 2751 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2996 3026 3271 Size: 30 165 30 777 30 786 30 245 Cuts at: 3271 3301 1178 Size: 30 4799 Fragments arranged by size: 4799 786 777 245 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2759 2825 3434 3719 4169 4181 1186 Size: 852 721 66 609 285 450 12 3927 Fragments arranged by size: 3927 852 721 609 450 285 66 12 BseYI C'CCAG_C Cuts at: 2903 6125 6621 2903 Size: 3222 496 3204 Fragments arranged by size: 3222 3204 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2152 3200 3752 3860 1163 Size: 573 416 1048 552 108 4225 Fragments arranged by size: 4225 1048 573 552 416 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 2975 4372 5133 6161 1540 Size: 275 186 37 937 1397 761 1028 2301 Fragments arranged by size: 2301 1397 1028 937 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 3444 3963 4983 5068 6139 3444 Size: 519 1020 85 1071 4227 Fragments arranged by size: 4227 1071 1020 519 85 BsmI GAATG_Cn' Cuts at: 4433 4526 4433 Size: 93 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2227 3148 3163 3444 3495 3963 4241 Size: 399 437 921 15 281 51 468 278 Cuts at: 4241 4983 5068 6139 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 921 742 468 437 399 281 278 85 51 15 BspEI T'CCGG_A Cuts at: 2604 6585 2604 Size: 3981 2941 BspHI T'CATG_A Cuts at: 4811 6541 4811 Size: 1730 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3222 3228 3370 4277 844 Size: 2378 6 142 907 3489 Fragments arranged by size: 3489 2378 907 142 6 BsrBI CCG'CTC Cuts at: 1394 1823 4809 1394 Size: 429 2986 3507 Fragments arranged by size: 3507 2986 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2914 5417 5591 65 Size: 1183 1666 2503 174 1396 Fragments arranged by size: 2503 1666 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5566 5753 1700 Size: 110 3756 187 2869 Fragments arranged by size: 3756 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2187 2424 96 Size: 2091 237 4594 Fragments arranged by size: 4594 2091 237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 3147 4982 5994 3147 Size: 1835 1012 4075 Fragments arranged by size: 4075 1835 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 5243 BstBI TT'CG_AA Cuts at: 5726 5726 Size: 6922 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstXI CCAn_nnnn'nTGG Cuts at: 2128 3899 4123 2128 Size: 1771 224 4927 Fragments arranged by size: 4927 1771 224 BstYI r'GATC_y Cuts at: 1972 2098 2607 2880 3231 4252 4619 5004 5021 Size: 126 509 273 351 1021 367 385 17 Cuts at: 5021 6473 6559 6571 6917 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1021 509 385 367 351 346 273 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3185 3185 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 2004 2032 2081 2264 2500 3930 513 Size: 552 939 28 49 183 236 1430 3505 Fragments arranged by size: 3505 1430 939 552 236 183 49 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2364 3225 3666 487 Size: 683 56 1138 861 441 3743 Fragments arranged by size: 3743 1138 861 683 441 56 BtsI GCAGTG_nn' Cuts at: 3209 3311 3585 4438 5232 5252 3209 Size: 102 274 853 794 20 4879 Fragments arranged by size: 4879 853 794 274 102 20 ClaI AT'CG_AT Cuts at: 1554 3861 1554 Size: 2307 4615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 4226 4226 Size: 6922 DraIII CAC_nnn'GTG Cuts at: 2185 2185 Size: 6922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4067 5929 809 Size: 507 2751 1862 1802 Fragments arranged by size: 2751 1862 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2566 2784 Size: 54 1425 50 275 18 205 531 218 Cuts at: 2784 2972 3360 3426 3915 4017 4369 5258 6887 Size: 188 388 66 489 102 352 889 1629 Cuts at: 6887 8 Size: 43 Fragments arranged by size: 1629 1425 889 531 489 388 352 275 218 205 188 102 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 2972 4369 1537 Size: 275 223 937 1397 4090 Fragments arranged by size: 4090 1397 937 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 2338 2627 2702 3612 4211 4852 1353 Size: 985 289 75 910 599 641 3423 Fragments arranged by size: 3423 985 910 641 599 289 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2498 2615 5509 5883 6029 387 Size: 1064 138 909 117 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 909 374 146 138 117 EcoICRI GAG'CTC Cuts at: 3961 3961 Size: 6922 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2426 2507 2774 2780 2798 3023 Size: 393 550 244 81 267 6 18 225 Cuts at: 3023 3218 3273 3422 3620 3731 3740 3902 4985 Size: 195 55 149 198 111 9 162 1083 Cuts at: 4985 5582 6369 6637 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1083 787 597 550 393 268 267 244 225 198 195 162 149 111 81 55 18 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3224 4070 3224 Size: 846 6076 EcoO109I rG'GnC_Cy Cuts at: 2300 3160 2300 Size: 860 6062 EcoRI G'AATT_C Cuts at: 4231 4609 4231 Size: 378 6544 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 2045 2264 2850 3469 6069 1678 Size: 367 219 586 619 2600 2531 Fragments arranged by size: 2600 2531 619 586 367 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2717 2749 2996 3028 3269 Size: 32 135 32 1145 32 247 32 241 Cuts at: 3269 3301 3644 3676 5563 5595 5637 5669 1373 Size: 32 343 32 1887 32 42 32 2626 Fragments arranged by size: 2626 1887 1145 343 247 241 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4054 4266 4512 669 Size: 988 281 2116 212 246 3079 Fragments arranged by size: 3079 2116 988 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 6922 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KasI G'GCGC_C Cuts at: 2041 2041 Size: 6922 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 4521 4521 Size: 6922 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6036 6220 1305 Size: 4731 184 2007 Fragments arranged by size: 4731 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 10 Size: 54 1425 343 954 4146 Fragments arranged by size: 4146 1425 954 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4880 5239 5398 518 Size: 648 3714 359 159 2042 Fragments arranged by size: 3714 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2083 2115 2470 2980 3196 3880 4338 Size: 428 49 32 355 510 216 684 458 Cuts at: 4338 5015 6163 6408 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 684 677 510 458 428 355 245 216 49 32 NarI GG'CG_CC Cuts at: 2042 2042 Size: 6922 NcoI C'CATG_G Cuts at: 513 1065 2004 2264 2500 513 Size: 552 939 260 236 4935 Fragments arranged by size: 4935 939 552 260 236 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NruI TCG'CGA Cuts at: 2349 2349 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 2548 2710 3157 3928 4280 5825 1132 Size: 97 1319 162 447 771 352 1545 2229 Fragments arranged by size: 2229 1545 1319 771 447 352 162 97 PasI CC'CwG_GG Cuts at: 1348 2128 2461 3163 1348 Size: 780 333 702 5107 Fragments arranged by size: 5107 780 702 333 PciI A'CATG_T Cuts at: 2706 5821 2706 Size: 3115 3807 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6922 PfoI T'CCnGG_A Cuts at: 2796 2883 3273 2796 Size: 87 390 6445 Fragments arranged by size: 6445 390 87 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2300 2300 Size: 6922 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PspOMI G'GGCC_C Cuts at: 3159 3159 Size: 6922 PstI C_TGCA'G Cuts at: 830 3132 3318 3417 3462 3567 3672 4274 830 Size: 2302 186 99 45 105 105 602 3478 Fragments arranged by size: 3478 2302 602 186 105 105 99 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 PvuII CAG'CTG Cuts at: 2115 2980 3196 3880 2115 Size: 865 216 684 5157 Fragments arranged by size: 5157 865 684 216 SacI G_AGCT'C Cuts at: 3963 3963 Size: 6922 SacII CC_GC'GG Cuts at: 2035 2084 2035 Size: 49 6873 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SapI GCTCTTCn'nnn_ Cuts at: 2702 4211 2702 Size: 1509 5413 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SfcI C'TryA_G Cuts at: 826 1045 3008 3128 3314 3413 3458 3563 3668 Size: 219 1963 120 186 99 45 105 105 Cuts at: 3668 3739 4270 5405 6086 6277 826 Size: 71 531 1135 681 191 1471 Fragments arranged by size: 1963 1471 1135 681 531 219 191 186 120 105 105 99 71 45 SfoI GGC'GCC Cuts at: 2043 2043 Size: 6922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 3260 4249 1872 Size: 1388 989 4545 Fragments arranged by size: 4545 1388 989 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 2738 3302 5026 5927 6189 Size: 197 926 432 363 564 1724 901 262 Cuts at: 6189 820 Size: 1553 Fragments arranged by size: 1724 1553 926 901 564 432 363 262 197 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 2548 3157 3928 4280 1132 Size: 1416 609 771 352 3774 Fragments arranged by size: 3774 1416 771 609 352 SspI AAT'ATT Cuts at: 5 52 4846 5 Size: 47 4794 2081 Fragments arranged by size: 4794 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6922 StyI C'CwwG_G Cuts at: 513 1065 2004 2264 2500 2838 3693 4361 513 Size: 552 939 260 236 338 855 668 3074 Fragments arranged by size: 3074 939 855 668 552 338 260 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 4949 4966 5119 5304 5749 325 Size: 1255 1965 1404 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1404 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2424 5168 Size: 274 80 33 51 1443 210 237 2744 Cuts at: 5168 6880 96 Size: 1712 138 Fragments arranged by size: 2744 1712 1443 274 237 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3271 3303 6882 6914 3271 Size: 32 3579 32 3279 Fragments arranged by size: 3579 3279 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2731 3557 4067 1316 Size: 1415 826 510 4171 Fragments arranged by size: 4171 1415 826 510 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2812 4120 2812 Size: 1308 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 3258 4247 1870 Size: 1388 989 4545 Fragments arranged by size: 4545 1388 989 XmnI GAAnn'nnTTC Cuts at: 2696 4101 5051 2696 Size: 1405 950 4567 Fragments arranged by size: 4567 1405 950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI