################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HindIII HinfI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TfiI TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 52 GACGTC GACGT^C AccII 547 CGCG CG^CG AcsI 373 AAATTC [GA]^AATT[CT] AfaI 684 GTAC GT^AC AocI 729 CCTCAGG CC^T[ACGT]AGG ApaI 154 GGGCCC GGGCC^C ApoI 373 AAATTC [GA]^AATT[CT] AspHI 244 GAGCTC G[AT]GC[AT]^C AspI 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 543 GGACC G^G[AT]CC BanI 294 GGTGCC G^G[CT][GA]CC BcnI 240 CCGGG CC^[GC]GG BfaI 421 CTAG C^TAG BpmI 592 CTCCAG CTGGAG(16/14) BseRI 364 GAGGAG GAGGAG(10/8) BsgI 70 GTGCAG GTGCAG(16/14) BsiHKAI 244 GAGCTC G[AT]GC[AT]^C BsrDI 535 GCAATG GCAATG(2/0) BssSI 604 CACGAG CACGAG(-5/-1) BstAPI 555 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 621 GGTCACC G^GT[ACGT]ACC BstPI 621 GGTCACC G^GT[ACGT]ACC BstUI 547 CGCG CG^CG BstYI 275 AGATCC [GA]^GATC[CT] Bsu36I 729 CCTCAGG CC^T[ACGT]AGG DdeI 730 CTCAG C^T[ACGT]AG Eco81I 729 CCTCAGG CC^T[ACGT]AGG EcoO65I 621 GGTCACC G^GT[ACGT]ACC EcoT14I 641 CCTTGG C^C[AT][AT]GG FauI 150 GCGGG CCCGC(4/6) HgaI 76 GCGTC GACGC(5/10) HincII 121 GTTAAC GT[CT]^[GA]AC HindII 121 GTTAAC GT[CT]^[GA]AC HpaI 121 GTTAAC GTT^AAC HphI 623 TCACC GGTGA(8/7) KspI 546 CCGCGG CCGC^GG MaeI 421 CTAG C^TAG MaeII 53 ACGT A^CGT MflI 275 AGATCC [GA]^GATC[CT] MseI 122 TTAA T^TAA MvnI 547 CGCG CG^CG NciI 240 CCGGG CC^[GC]GG NheI 420 GCTAGC G^CTAGC NspI 551 GCATGC [GA]CATG^[CT] PflFI 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 432 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 487 CTGCAG CTGCA^G RsaI 684 GTAC GT^AC SacI 244 GAGCTC GAGCT^C SacII 546 CCGCGG CCGC^GG SapI 246 GCTCTTC GCTCTTC(1/4) SfaNI 586 GATGC GCATC(5/9) SfcI 487 CTGCAG C^T[GA][CT]AG SphI 551 GCATGC GCATG^C StuI 389 AGGCCT AGG^CCT StyI 641 CCTTGG C^C[AT][AT]GG Tru9I 122 TTAA T^TAA Tsp509I 374 AATT ^AATT Tth111I 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 434 CCATTGTCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 275 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 52 GACGTC G[GA]^CG[CT]C 75 GGCGTC AlwI 237 GATCC GGATC(4/5) 276 GATCC BanII 154 GGGCCC G[GA]GC[CT]^C 244 GAGCTC BsaHI 52 GACGTC G[GA]^CG[CT]C 75 GGCGTC BspMI 146 ACCTGC ACCTGC(4/8) 332 GCAGGT BsrI 118 CCAGT ACTGG(1/-1) 678 CCAGT BstF5I 402 GGATG GGATG(2/0) 702 GGATG DraII 153 GGGGCCC [GA]G^G[ACGT]CC[CT] 154 GGGCCCC DsaI 59 CCACGG C^C[GA][CT]GG 546 CCGCGG EcoO109I 153 GGGGCCC [GA]G^G[ACGT]CC[CT] 154 GGGCCCC FokI 402 GGATG GGATG(9/13) 702 GGATG HapII 240 CCGG C^CGG 700 CCGG Hin1I 52 GACGTC G[GA]^CG[CT]C 75 GGCGTC HpaII 240 CCGG C^CGG 700 CCGG MspI 240 CCGG C^CGG 700 CCGG TspRI 209 CACTG CA[GC]TG[ACGT][ACGT]^ 327 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI HaeIII ItaI Ksp632I MaeIII MboI MboII MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I