Restriction map for
pFN21AE2160
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccII AciI AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsgI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MboI
MfeI MflI MluI MseI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 652 GTCTAC GT^[AC][GT]AC
AccIII 222 TCCGGA T^CCGGA
Alw44I 318 GTGCAC G^TGCAC
AlwNI 62 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 318 GTGCAC G^TGCAC
BalI 290 TGGCCA TGG^CCA
BanII 536 GAGCTC G[GA]GC[CT]^C
BpmI 657 CTCCAG CTGGAG(16/14)
BsaBI 288 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 222 TCCGGA T^CCGGA
BseRI 533 GAGGAG GAGGAG(10/8)
BsmFI 326 GGGAC GGGAC(10/14)
BspEI 222 TCCGGA T^CCGGA
BspHI 284 TCATGA T^CATGA
BsrI 516 ACTGG ACTGG(1/-1)
BssKI 314 CCAGG ^CC[ACGT]GG
BstNI 314 CCAGG CC^[AT]GG
BstXI 509 CCACGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 312 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 509 CCACGG C^C[GA][CT]GG
EaeI 290 TGGCCA [CT]^GGCC[GA]
EcoRII 314 CCAGG ^CC[AT]GG
EcoT14I 297 CCTTGG C^C[AT][AT]GG
HgaI 650 GCGTC GACGC(5/10)
MaeII 474 ACGT A^CGT
MamI 288 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 374 TCTTC GAAGA(8/7)
MluNI 290 TGGCCA TGG^CCA
MroI 222 TCCGGA T^CCGGA
MscI 290 TGGCCA TGG^CCA
MvaI 314 CCAGG CC^[AT]GG
RcaI 284 TCATGA T^CATGA
SacI 536 GAGCTC GAGCT^C
ScrFI 314 CCAGG CC^[ACGT]GG
SexAI 313 ACCAGGT A^CC[AT]GGT
SfaNI 183 GATGC GCATC(5/9)
StyI 297 CCTTGG C^C[AT][AT]GG
TaqI 592 TCGA T^CGA
Tsp509I 157 AATT ^AATT
TthHB8I 592 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 225 GGACC G^G[AT]CC
506 GGACC
BsaJI 297 CCTTGG C^C[ACGT][ACGT]GG
509 CCACGG
BsaWI 222 TCCGGA [AT]^CCGG[AT]
631 ACCGGA
Cac8I 168 GCAGGC GC[ACGT]^[ACGT]GC
210 GCTGGC
Cfr13I 225 GGACC G^G[ACGT]CC
506 GGACC
HaeIII 99 GGCC GG^CC
291 GGCC
HapII 223 CCGG C^CGG
632 CCGG
HinfI 202 GATTC G^A[ACGT]TC
368 GAATC
HpaII 223 CCGG C^CGG
632 CCGG
HphI 339 GGTGA GGTGA(8/7)
643 TCACC
MaeIII 70 GTCAC ^GT[ACGT]AC
642 GTCAC
MspI 223 CCGG C^CGG
632 CCGG
Sau96I 225 GGACC G^G[ACGT]CC
506 GGACC
SfcI 229 CTACAG C^T[GA][CT]AG
582 CTACAG
TfiI 202 GATTC G^A[AT]TC
368 GAATC
Tsp45I 70 GTCAC ^GT[GC]AC
642 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsiHKAI
Bsp1286I BstAPI BstF5I DdeI Fnu4HI
FokI ItaI MnlI MslI MwoI
NlaIII TseI TspRI