################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 255 GTAC GT^AC AlwI 510 GATCC GGATC(4/5) ApaI 562 GGGCCC GGGCC^C BciVI 180 GGATAC GTATCC(6/5) BcnI 311 CCCGG CC^[GC]GG BpmI 239 CTGGAG CTGGAG(16/14) BseRI 64 CTCCTC GAGGAG(10/8) BsrDI 461 GCAATG GCAATG(2/0) BsrI 596 CCAGT ACTGG(1/-1) BssKI 311 CCCGG ^CC[ACGT]GG BssSI 617 CTCGTG CACGAG(-5/-1) BstYI 509 AGATCC [GA]^GATC[CT] Cac8I 457 GCCTGC GC[ACGT]^[ACGT]GC DpnII 510 GATC ^GATC FauI 374 CCCGC CCCGC(4/6) HapII 312 CCGG C^CGG HincII 142 GTCAAC GT[CT]^[GA]AC HindII 142 GTCAAC GT[CT]^[GA]AC HpaII 312 CCGG C^CGG HphI 212 TCACC GGTGA(8/7) MboI 510 GATC ^GATC MflI 509 AGATCC [GA]^GATC[CT] MseI 482 TTAA T^TAA MslI 42 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 312 CCGG C^CGG NciI 311 CCCGG CC^[GC]GG NdeII 510 GATC ^GATC PleI 370 GAGTC GAGTC(4/5) PpuMI 591 GGGACCC [GA]G^G[AT]CC[CT] PstI 70 CTGCAG CTGCA^G PvuII 236 CAGCTG CAG^CTG RsaI 255 GTAC GT^AC SacI 131 GAGCTC GAGCT^C Sau3AI 510 GATC ^GATC ScrFI 311 CCCGG CC^[ACGT]GG TfiI 196 GAATC G^A[AT]TC Tru9I 482 TTAA T^TAA Tsp509I 576 AATT ^AATT XhoII 509 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AhdI 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 622 GACATAGGGTC AluI 132 AGCT AG^CT 237 AGCT AspEI 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 622 GACATAGGGTC AspHI 131 GAGCTC G[AT]GC[AT]^C 242 GAGCAC AvaII 592 GGACC G^G[AT]CC 629 GGTCC BanII 131 GAGCTC G[GA]GC[CT]^C 562 GGGCCC BsiHKAI 131 GAGCTC G[AT]GC[AT]^C 242 GAGCAC BsmAI 38 GAGAC GTCTC(1/5) 62 GTCTC BsmFI 591 GGGAC GGGAC(10/14) 372 GTCCC BstF5I 306 GGATG GGATG(2/0) 381 GGATG DraII 562 GGGCCCC [GA]G^G[ACGT]CC[CT] 591 GGGACCC Eam1105I 364 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 622 GACATAGGGTC EcoNI 221 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 407 CCTTTGCTAGG EcoO109I 562 GGGCCCC [GA]G^G[ACGT]CC[CT] 591 GGGACCC FokI 306 GGATG GGATG(9/13) 381 GGATG HinfI 196 GAATC G^A[ACGT]TC 370 GAGTC MaeIII 362 GTGAC ^GT[ACGT]AC 620 GTGAC MspA1I 236 CAGCTG C[AC]G^C[GT]G 607 CAGCGG SfaNI 307 GATGC GCATC(5/9) 551 GCATC SfcI 70 CTGCAG C^T[GA][CT]AG 225 CTACAG StuI 404 AGGCCT AGG^CCT 473 AGGCCT Tsp45I 362 GTGAC ^GT[GC]AC 620 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI BbvI BfaI BmyI BsiYI BslI Bsp1286I Cfr13I EarI Fnu4HI HaeIII ItaI Ksp632I MaeI MboII MnlI MwoI NlaIII NlaIV Sau96I TseI TspRI