Restriction map for
pFN21AE5226
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HapII HgaI Hin1I HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeI MaeII MamI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 137 GTCGAC GT^[AC][GT]AC
AlwNI 116 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 209 GGGCCC GGGCC^C
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
Asp700I 289 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 455 GAGCAC G[AT]GC[AT]^C
AvaII 228 GGTCC G^G[AT]CC
BanII 209 GGGCCC G[GA]GC[CT]^C
BsiHKAI 455 GAGCAC G[AT]GC[AT]^C
BspMI 325 GCAGGT ACCTGC(4/8)
BstYI 422 AGATCC [GA]^GATC[CT]
Cac8I 522 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 4 GCGC GCG^C
DdeI 433 CTAAG C^T[ACGT]AG
DraII 208 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 308 GACTTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 208 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 101 CCTTGG C^C[AT][AT]GG
EcoT22I 358 ATGCAT ATGCA^T
FauI 6 GCGGG CCCGC(4/6)
HaeIII 210 GGCC GG^CC
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
MaeIII 112 GTGAC ^GT[ACGT]AC
MfeI 320 CAATTG C^AATTG
MflI 422 AGATCC [GA]^GATC[CT]
MseI 389 TTAA T^TAA
MspA1I 395 CAGCTG C[AC]G^C[GT]G
MunI 320 CAATTG C^AATTG
NsiI 358 ATGCAT ATGCA^T
PshBI 388 ATTAAT AT^TAAT
PvuII 395 CAGCTG CAG^CTG
SalI 137 GTCGAC G^TCGAC
StyI 101 CCTTGG C^C[AT][AT]GG
Tru9I 389 TTAA T^TAA
Tsp45I 112 GTGAC ^GT[GC]AC
Tsp509I 321 AATT ^AATT
XhoII 422 AGATCC [GA]^GATC[CT]
XmnI 289 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 5 CGCG CG^CG
27 CGCG
AlwI 423 GATCC GGATC(4/5)
105 GGATC
BmyI 209 GGGCCC G[AGT]GC[ACT]^C
455 GAGCAC
BpmI 350 CTGGAG CTGGAG(16/14)
517 CTGGAG
BsiYI 238 CCTGAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
319 CCAATTGCAGG
BslI 238 CCTGAACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
319 CCAATTGCAGG
BsmFI 306 GGGAC GGGAC(10/14)
229 GTCCC
BsmI 30 GAATGC GAATGC(1/-1)
356 GAATGC
Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C
455 GAGCAC
BsrI 516 ACTGG ACTGG(1/-1)
469 CCAGT
BstF5I 298 CATCC GGATG(2/0)
484 CATCC
BstUI 5 CGCG CG^CG
27 CGCG
DpnII 106 GATC ^GATC
423 GATC
EarI 233 CTCTTC CTCTTC(1/4)
439 GAAGAG
FokI 298 CATCC GGATG(9/13)
484 CATCC
HincII 137 GTCGAC GT[CT]^[GA]AC
265 GTCAAC
HindII 137 GTCGAC GT[CT]^[GA]AC
265 GTCAAC
HinfI 140 GACTC G^A[ACGT]TC
503 GAGTC
Ksp632I 233 CTCTTC CTCTTC(1/4)
439 GAAGAG
MboI 106 GATC ^GATC
423 GATC
MslI 156 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
372 CATCGAGGTG
MvnI 5 CGCG CG^CG
27 CGCG
MwoI 19 GCTGCAGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
25 GCCGCGAATGC
NdeII 106 GATC ^GATC
423 GATC
PleI 140 GACTC GAGTC(4/5)
503 GAGTC
Sau3AI 106 GATC ^GATC
423 GATC
SfaNI 360 GCATC GCATC(5/9)
371 GCATC
SspI 199 AATATT AAT^ATT
385 AATATT
TaqI 138 TCGA T^CGA
374 TCGA
TspRI 161 CAGTG CA[GC]TG[ACGT][ACGT]^
470 CAGTG
TthHB8I 138 TCGA T^CGA
374 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI Cfr13I EcoRII Fnu4HI ItaI
MboII MnlI MvaI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI