Restriction map for
pFN21AE5226
(Linear) (Six-base) MAPSORT of: KIEE5226.seq Check: 8244 from: 1 to: 528
KIEE5226.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AccI GT'mk_AC
Cuts at: 0 138 528
Size: 138 390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 528
Size: 34 494
AlwNI CAG_nnn'CTG
Cuts at: 0 121 528
Size: 121 407
ApaI G_GGCC'C
Cuts at: 0 213 528
Size: 213 315
AseI AT'TA_AT
Cuts at: 0 389 528
Size: 389 139
BanII G_rGCy'C
Cuts at: 0 213 528
Size: 213 315
BfrBI ATG'CAT
Cuts at: 0 360 528
Size: 360 168
Bme1580I G_kGCm'C
Cuts at: 0 213 528
Size: 213 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 528
Size: 371 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 239 269 494 524 528
Size: 239 30 225 30 4
Fragments arranged by size:
239 225 30 30 4
BsiHKAI G_wGCw'C
Cuts at: 0 459 528
Size: 459 69
BsmI GAATG_Cn'
Cuts at: 0 36 362 528
Size: 36 326 166
Fragments arranged by size:
326 166 36
Bsp1286I G_dGCh'C
Cuts at: 0 213 459 528
Size: 213 246 69
Fragments arranged by size:
246 213 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 528
Size: 316 212
BstYI r'GATC_y
Cuts at: 0 422 528
Size: 422 106
BtsI GCAGTG_nn'
Cuts at: 0 167 528
Size: 167 361
DrdI GACnn_nn'nnGTC
Cuts at: 0 314 528
Size: 314 214
EarI CTCTTCn'nnn_
Cuts at: 0 239 434 528
Size: 239 195 94
Fragments arranged by size:
239 195 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 371 528
Size: 34 337 157
Fragments arranged by size:
337 157 34
EcoO109I rG'GnC_Cy
Cuts at: 0 209 528
Size: 209 319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 101 528
Size: 101 427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 414 446 528
Size: 414 32 82
Fragments arranged by size:
414 82 32
HincII GTy'rAC
Cuts at: 0 139 267 528
Size: 139 128 261
Fragments arranged by size:
261 139 128
MfeI C'AATT_G
Cuts at: 0 320 528
Size: 320 208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 528
Size: 94 434
MslI CAynn'nnrTG
Cuts at: 0 160 376 528
Size: 160 216 152
Fragments arranged by size:
216 160 152
MspA1I CmG'CkG
Cuts at: 0 397 528
Size: 397 131
NsiI A_TGCA'T
Cuts at: 0 362 528
Size: 362 166
PsiI TTA'TAA
Cuts at: 0 194 528
Size: 194 334
PspOMI G'GGCC_C
Cuts at: 0 209 528
Size: 209 319
PstI C_TGCA'G
Cuts at: 0 24 186 338 528
Size: 24 162 152 190
Fragments arranged by size:
190 162 152 24
PvuII CAG'CTG
Cuts at: 0 397 528
Size: 397 131
SalI G'TCGA_C
Cuts at: 0 137 528
Size: 137 391
SfcI C'TryA_G
Cuts at: 0 20 182 334 528
Size: 20 162 152 194
Fragments arranged by size:
194 162 152 20
SspI AAT'ATT
Cuts at: 0 201 387 528
Size: 201 186 141
Fragments arranged by size:
201 186 141
StyI C'CwwG_G
Cuts at: 0 101 528
Size: 101 427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 494 526 528
Size: 455 32 7 32 2
Fragments arranged by size:
455 32 32 7 2
XmnI GAAnn'nnTTC
Cuts at: 0 293 528
Size: 293 235
Enzymes that do cut:
AccI AcuI AlwNI ApaI AseI BanII BfrBI Bme1580I
BpmI BsaXI BsiHKAI BsmI Bsp1286I BspMI BstYI BtsI
DrdI EarI Eco57MI EcoO109I FalI Hin4I HincII MfeI
MmeI MslI MspA1I NsiI PsiI PspOMI PstI PvuII
SalI SfcI SspI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI ZraI