################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TseI Tsp45I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 249 GTATAC GT^[AC][GT]AC AfaI 76 GTAC GT^AC AflII 66 CTTAAG C^TTAAG Asp700I 528 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 833 CTCGAG C^[CT]CG[GA]G BamHI 194 GGATCC G^GATCC BbsI 790 GAAGAC GAAGAC(2/6) BfrI 66 CTTAAG C^TTAAG BmyI 205 GTGCCC G[AGT]GC[ACT]^C BpuAI 790 GAAGAC GAAGAC(2/6) BsaAI 77 TACGTG [CT]AC^GT[GA] BsmFI 755 GGGAC GGGAC(10/14) BsoBI 833 CTCGAG C^[CT]CG[GA]G Bsp1286I 205 GTGCCC G[AGT]GC[ACT]^C BspHI 356 TCATGA T^CATGA Bst1107I 249 GTATAC GTA^TAC BstZ17I 249 GTATAC GTA^TAC Cfr13I 780 GGCCC G^G[ACGT]CC DraIII 257 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 847 CCATGG C^C[GA][CT]GG MaeIII 540 GTTAC ^GT[ACGT]AC MslI 496 CACATTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 847 CCATGG C^CATGG NlaIV 194 GGATCC GG[ACGT]^[ACGT]CC PaeR7I 833 CTCGAG C^TCGAG PleI 273 GAGTC GAGTC(4/5) RcaI 356 TCATGA T^CATGA RsaI 76 GTAC GT^AC Sau96I 780 GGCCC G^G[ACGT]CC TaqI 834 TCGA T^CGA TfiI 156 GATTC G^A[AT]TC TthHB8I 834 TCGA T^CGA XcmI 368 CCAGAGATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 833 CTCGAG C^TCGAG XmnI 528 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BglII 590 AGATCT A^GATCT 825 AGATCT BssKI 227 CCTGG ^CC[ACGT]GG 603 CCTGG BstNI 227 CCTGG CC^[AT]GG 603 CCTGG BstXI 427 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 495 CCACATTCATGG EcoRII 227 CCTGG ^CC[AT]GG 603 CCTGG HaeIII 339 GGCC GG^CC 780 GGCC HinfI 156 GATTC G^A[ACGT]TC 273 GAGTC HphI 345 GGTGA GGTGA(8/7) 223 TCACC MvaI 227 CCTGG CC^[AT]GG 603 CCTGG ScrFI 227 CCTGG CC^[ACGT]GG 603 CCTGG SspI 114 AATATT AAT^ATT 443 AATATT TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^ 776 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AluI AlwI BsaJI BseRI BsiYI BslI BsrI BstF5I BstYI DdeI DpnII EcoT14I FokI MaeII MboI MboII MflI MnlI MseI NdeII NlaIII Sau3AI SfaNI SmlI StyI Tru9I Tsp509I XhoII