Restriction map for
pFN21AE4393
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI MvaI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AciI 879 CCGC CCGC(-3/-1)
AcsI 357 GAATTT [GA]^AATT[CT]
AlwNI 337 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 357 GAATTT [GA]^AATT[CT]
AseI 133 ATTAAT AT^TAAT
AsnI 133 ATTAAT AT^TAAT
Asp700I 192 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 658 GAAGAC GAAGAC(2/6)
BglII 1165 AGATCT A^GATCT
BmyI 703 GTGCCC G[AGT]GC[ACT]^C
BpuAI 658 GAAGAC GAAGAC(2/6)
BsaWI 161 ACCGGA [AT]^CCGG[AT]
Bsp1286I 703 GTGCCC G[AGT]GC[ACT]^C
Cfr13I 248 GGGCC G^G[ACGT]CC
DraI 616 TTTAAA TTT^AAA
DraII 247 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 736 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 400 CTCTTC CTCTTC(1/4)
EcoO109I 247 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeIII 249 GGCC GG^CC
HapII 162 CCGG C^CGG
HincII 901 GTTGAC GT[CT]^[GA]AC
HindII 901 GTTGAC GT[CT]^[GA]AC
HpaII 162 CCGG C^CGG
Ksp632I 400 CTCTTC CTCTTC(1/4)
MspA1I 1094 CAGCTG C[AC]G^C[GT]G
MspI 162 CCGG C^CGG
NdeI 938 CATATG CA^TATG
NspI 978 GCATGC [GA]CATG^[CT]
PshBI 133 ATTAAT AT^TAAT
PvuII 1094 CAGCTG CAG^CTG
Sau96I 248 GGGCC G^G[ACGT]CC
SmlI 574 CTCAAG C^T[CT][GA]AG
SphI 978 GCATGC GCATG^C
Tsp45I 3 GTCAC ^GT[GC]AC
XmnI 192 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 31 GTAC GT^AC
159 GTAC
BfaI 739 CTAG C^TAG
1216 CTAG
BglI 622 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCCATAAGGC
BsaI 178 GAGACC GGTCTC(1/5)
280 GAGACC
BsgI 759 CTGCAC GTGCAG(16/14)
1063 CTGCAC
BsiYI 12 CCTGTATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCCAAGGAGGG
BslI 12 CCTGTATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
814 CCCAAGGAGGG
BsmFI 822 GGGAC GGGAC(10/14)
1099 GTCCC
BstYI 320 AGATCC [GA]^GATC[CT]
1165 AGATCT
DdeI 269 CTGAG C^T[ACGT]AG
597 CTTAG
HinfI 832 GAGTC G^A[ACGT]TC
1010 GACTC
MaeI 739 CTAG C^TAG
1216 CTAG
MaeIII 3 GTCAC ^GT[ACGT]AC
79 GTAAC
MflI 320 AGATCC [GA]^GATC[CT]
1165 AGATCT
PleI 1010 GACTC GAGTC(4/5)
832 GAGTC
PshAI 465 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1021 GACAATGGTC
RsaI 31 GTAC GT^AC
159 GTAC
XhoII 320 AGATCC [GA]^GATC[CT]
1165 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsmAI
BsmI BsrI BstF5I Cac8I DpnII
EcoT14I Fnu4HI FokI HphI ItaI
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI SfaNI SfcI StyI Tru9I
TseI Tsp509I TspRI