(Circular) (Six-base) MAPSORT of: pFN21AE4393.seq Check: 3880 from: 1 to: 5950 pFN21AE4393 5950 bp With 182 enzymes: SgfI * March 28, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2493 2891 3111 3129 4013 5397 5665 Size: 462 399 398 220 18 884 1384 268 Cuts at: 5665 1632 Size: 1917 Fragments arranged by size: 1917 1384 884 462 399 398 268 220 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 3075 1166 Size: 1909 4041 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2789 1184 Size: 1605 4345 AlwNI CAG_nnn'CTG Cuts at: 2347 3693 5265 2347 Size: 1346 1572 3032 Fragments arranged by size: 3032 1572 1346 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 2362 3259 3485 3637 5746 1493 Size: 869 897 226 152 2109 1697 Fragments arranged by size: 2109 1697 897 869 226 152 AseI AT'TA_AT Cuts at: 160 1032 2139 4504 160 Size: 872 1107 2365 1606 Fragments arranged by size: 2365 1606 1107 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 4726 618 Size: 325 217 627 1484 1455 1842 Fragments arranged by size: 1842 1484 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3269 1391 Size: 1878 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2670 928 Size: 971 771 4208 Fragments arranged by size: 4208 971 771 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2633 3059 4561 Size: 107 122 71 631 605 961 426 1502 Cuts at: 4561 136 Size: 1525 Fragments arranged by size: 1525 1502 961 631 605 426 122 107 71 BglII A'GATC_T Cuts at: 3170 5945 3170 Size: 2775 3175 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2712 4011 5167 1790 Size: 922 1299 1156 2573 Fragments arranged by size: 2573 1299 1156 922 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3681 4639 Size: 416 53 83 399 24 1206 1058 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1206 1058 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4610 1239 Size: 3371 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4075 4940 5238 2564 Size: 1511 865 298 3276 Fragments arranged by size: 3276 1511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2177 2279 4613 882 Size: 617 678 102 2334 2219 Fragments arranged by size: 2334 2219 678 617 102 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2166 4383 4781 5055 5202 5613 2166 Size: 2217 398 274 147 411 2503 Fragments arranged by size: 2503 2217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2296 2326 1178 Size: 30 165 30 893 30 4802 Fragments arranged by size: 4802 893 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5950 BseYI C'CCAG_C Cuts at: 5153 5649 5153 Size: 496 5454 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2749 3053 1163 Size: 573 1013 304 4060 Fragments arranged by size: 4060 1013 573 304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 4096 5167 4011 Size: 85 1071 4794 Fragments arranged by size: 4794 1071 85 BsmI GAATG_Cn' Cuts at: 2153 2800 2976 3461 3554 2153 Size: 647 176 485 93 4549 Fragments arranged by size: 4549 647 485 176 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2712 3269 4011 4096 5167 1391 Size: 399 922 557 742 85 1071 2174 Fragments arranged by size: 2174 1071 922 742 557 399 85 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3837 1394 Size: 429 2014 3507 Fragments arranged by size: 3507 2014 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstYI r'GATC_y Cuts at: 1972 2325 3170 3280 3647 4032 4049 5501 5587 Size: 353 845 110 367 385 17 1452 86 Cuts at: 5587 5599 5945 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 845 385 367 353 346 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 2623 3254 2623 Size: 631 5319 DraIII CAC_nnn'GTG Cuts at: 2746 2746 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915 Size: 54 1425 50 275 18 1567 889 1629 Cuts at: 5915 8 Size: 43 Fragments arranged by size: 1629 1567 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 3880 1353 Size: 1058 1469 3423 Fragments arranged by size: 3423 1469 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2493 2891 3111 3129 4013 4610 Size: 393 462 399 398 220 18 884 597 Cuts at: 4610 5397 5665 1239 Size: 787 268 1524 Fragments arranged by size: 1524 884 787 597 462 399 398 393 268 220 18 EcoO109I rG'GnC_Cy Cuts at: 2253 2253 Size: 5950 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2218 3134 2218 Size: 916 5034 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2512 2544 4591 4623 4665 Size: 32 135 32 940 32 2047 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 2047 940 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2908 3294 3540 669 Size: 988 281 970 386 246 3079 Fragments arranged by size: 3079 988 970 386 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2520 5064 5248 1305 Size: 1215 2544 184 2007 Fragments arranged by size: 2544 2007 1215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4128 Fragments arranged by size: 4128 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2594 3075 3908 4267 4426 518 Size: 648 1149 279 481 833 359 159 2042 Fragments arranged by size: 2042 1149 833 648 481 359 279 159 MspA1I CmG'CkG Cuts at: 1606 3101 3366 4043 5191 5436 1606 Size: 1495 265 677 1148 245 2120 Fragments arranged by size: 2120 1495 1148 677 265 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5398 NdeI CA'TA_TG Cuts at: 387 2944 387 Size: 2557 3393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2987 3308 4853 1132 Size: 97 1758 321 1545 2229 Fragments arranged by size: 2229 1758 1545 321 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2474 3030 1613 Size: 861 556 4533 Fragments arranged by size: 4533 861 556 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2156 2188 2156 Size: 32 5918 PstI C_TGCA'G Cuts at: 830 2230 2264 2509 3100 3302 830 Size: 1400 34 245 591 202 3478 Fragments arranged by size: 3478 1400 591 245 202 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 3101 3101 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2226 2260 2505 2785 2891 3096 3298 Size: 219 1181 34 245 280 106 205 202 Cuts at: 3298 4433 5114 5305 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 681 280 245 219 205 202 191 106 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 4054 4955 5217 820 Size: 197 926 636 1475 901 262 1553 Fragments arranged by size: 1553 1475 926 901 636 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 2987 3308 1132 Size: 1855 321 3774 Fragments arranged by size: 3774 1855 321 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2289 2820 2950 3389 513 Size: 552 1224 531 130 439 3074 Fragments arranged by size: 3074 1224 552 531 439 130 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 3977 3994 4147 4332 4777 325 Size: 1255 702 1695 17 153 185 445 1498 Fragments arranged by size: 1695 1498 1255 702 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2296 2328 5910 5942 2296 Size: 32 3582 32 2304 Fragments arranged by size: 3582 2304 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2201 4079 2201 Size: 1878 4072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI