(Linear) (Six-base) MAPSORT of: KIEE4393.seq  Check: 4575  from: 1  to: 1245



KIEE4393.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     488     886    1106    1124    1245 
   Size:         89     399     398     220      18     121

  Fragments arranged by size: 

                 399     398     220     121      89      18
 
AleI CACnn'nnGTG

Cuts at:      0    1070    1245 
   Size:       1070     175
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     784    1245 
   Size:        784     461
 
AlwNI CAG_nnn'CTG

Cuts at:      0     342    1245 
   Size:        342     903
 
ApoI r'AATT_y

Cuts at:      0     357    1245 
   Size:        357     888
 
AseI AT'TA_AT

Cuts at:      0     134    1245 
   Size:        134    1111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     665    1245 
   Size:        665     580
 
BglI GCCn_nnn'nGGC

Cuts at:      0     628    1054    1245 
   Size:        628     426     191

  Fragments arranged by size: 

                 628     426     191
 
BglII A'GATC_T

Cuts at:      0    1165    1245 
   Size:       1165      80
 
Bme1580I G_kGCm'C

Cuts at:      0     707    1245 
   Size:        707     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     618    1245 
   Size:        618     627
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    1245 
   Size:        559     686
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     172     274    1245 
   Size:        172     102     971

  Fragments arranged by size: 

                 971     172     102
 
BsaWI w'CCGG_w

Cuts at:      0     161    1245 
   Size:        161    1084
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     291     321    1245 
   Size:        291      30     924

  Fragments arranged by size: 

                 924     291      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     744    1048    1245 
   Size:        744     304     197

  Fragments arranged by size: 

                 744     304     197
 
BsmI GAATG_Cn'

Cuts at:      0     148     795     971    1245 
   Size:        148     647     176     274

  Fragments arranged by size: 

                 647     274     176     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     707    1245 
   Size:        707     538
 
BstYI r'GATC_y

Cuts at:      0     320    1165    1245 
   Size:        320     845      80

  Fragments arranged by size: 

                 845     320      80
 
DraI TTT'AAA

Cuts at:      0     618    1245 
   Size:        618     627
 
DraIII CAC_nnn'GTG

Cuts at:      0     741    1245 
   Size:        741     504
 
EarI CTCTTCn'nnn_

Cuts at:      0     406    1245 
   Size:        406     839
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     488     886    1106    1124    1245 
   Size:         89     399     398     220      18     121

  Fragments arranged by size: 

                 399     398     220     121      89      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     248    1245 
   Size:        248     997
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     213    1129    1245 
   Size:        213     916     116

  Fragments arranged by size: 

                 916     213     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539    1245 
   Size:        507      32     706

  Fragments arranged by size: 

                 706     507      32
 
HincII GTy'rAC

Cuts at:      0     903    1245 
   Size:        903     342
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1245 
   Size:        515     730
 
MslI CAynn'nnrTG

Cuts at:      0     310     589    1070    1245 
   Size:        310     279     481     175

  Fragments arranged by size: 

                 481     310     279     175
 
MspA1I CmG'CkG

Cuts at:      0    1096    1245 
   Size:       1096     149
 
NdeI CA'TA_TG

Cuts at:      0     939    1245 
   Size:        939     306
 
NspI r_CATG'y

Cuts at:      0     982    1245 
   Size:        982     263
 
PshAI GACnn'nnGTC

Cuts at:      0     469    1025    1245 
   Size:        469     556     220

  Fragments arranged by size: 

                 556     469     220
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     151     183    1245 
   Size:        151      32    1062

  Fragments arranged by size: 

                1062     151      32
 
PstI C_TGCA'G

Cuts at:      0     225     259     504    1095    1245 
   Size:        225      34     245     591     150

  Fragments arranged by size: 

                 591     245     225     150      34
 
PvuII CAG'CTG

Cuts at:      0    1096    1245 
   Size:       1096     149
 
SfcI C'TryA_G

Cuts at:      0     221     255     500     780     886    1091    1245 
   Size:        221      34     245     280     106     205     154

  Fragments arranged by size: 

                 280     245     221     205     154     106      34
 
SmlI C'TyrA_G

Cuts at:      0     574    1245 
   Size:        574     671
 
SphI G_CATG'C

Cuts at:      0     982    1245 
   Size:        982     263
 
StyI C'CwwG_G

Cuts at:      0     284     815     945    1245 
   Size:        284     531     130     300

  Fragments arranged by size: 

                 531     300     284     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     277    1245 
   Size:        277     968
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     291     323    1245 
   Size:        291      32     922

  Fragments arranged by size: 

                 922     291      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     196    1245 
   Size:        196    1049
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApoI     AseI     BbsI     BglI
    BglII Bme1580I     BmrI    BpuEI     BsaI    BsaWI    BsaXI     BsgI
     BsmI Bsp1286I    BstYI     DraI   DraIII     EarI  Eco57MI EcoO109I
     FalI    Hin4I   HincII     MmeI     MslI   MspA1I     NdeI     NspI
    PshAI     PsrI     PstI    PvuII     SfcI     SmlI     SphI     StyI
    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI