Restriction map for
pFN21AE4315
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tru9I Tth111I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 853 GAATTC [GA]^AATT[CT]
AcyI 378 GACGCC G[GA]^CG[CT]C
AfaI 549 GTAC GT^AC
Alw44I 258 GTGCAC G^TGCAC
ApaLI 258 GTGCAC G^TGCAC
ApoI 853 GAATTC [GA]^AATT[CT]
BamHI 3 GGATCC G^GATCC
BanI 64 GGCACC G^G[CT][GA]CC
BbsI 229 GAAGAC GAAGAC(2/6)
BfaI 886 CTAG C^TAG
BpuAI 229 GAAGAC GAAGAC(2/6)
BsaHI 378 GACGCC G[GA]^CG[CT]C
BseRI 172 GAGGAG GAGGAG(10/8)
BsiYI 49 CCCGTATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 49 CCCGTATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 376 GAGAC GTCTC(1/5)
BsmBI 376 GAGACG CGTCTC(1/5)
BspDI 908 ATCGAT AT^CGAT
BspMI 177 GCAGGT ACCTGC(4/8)
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
Cac8I 130 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 771 GCGC GCG^C
ClaI 908 ATCGAT AT^CGAT
DdeI 729 CTTAG C^T[ACGT]AG
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 222 GAAGAG CTCTTC(1/4)
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
EcoRI 853 GAATTC G^AATTC
EcoT14I 293 CCTTGG C^C[AT][AT]GG
HgaI 378 GACGC GACGC(5/10)
HhaI 771 GCGC GCG^C
Hin1I 378 GACGCC G[GA]^CG[CT]C
HinP1I 771 GCGC G^CGC
HindIII 250 AAGCTT A^AGCTT
Ksp632I 222 GAAGAG CTCTTC(1/4)
MaeI 886 CTAG C^TAG
MslI 486 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 542 GACTC GAGTC(4/5)
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
PstI 781 CTGCAG CTGCA^G
RsaI 549 GTAC GT^AC
SexAI 566 ACCAGGT A^CC[AT]GGT
SfaNI 466 GATGC GCATC(5/9)
SspI 411 AATATT AAT^ATT
StyI 293 CCTTGG C^C[AT][AT]GG
TfiI 148 GATTC G^A[AT]TC
XbaI 885 TCTAGA T^CTAGA
XcmI 777 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 138 CCGC CCGC(-3/-1)
825 GCGG
AspHI 258 GTGCAC G[AT]GC[AT]^C
650 GTGCTC
BmyI 258 GTGCAC G[AGT]GC[ACT]^C
650 GTGCTC
BpmI 77 CTCCAG CTGGAG(16/14)
517 CTGGAG
BsaJI 88 CCGAGG C^C[ACGT][ACGT]GG
293 CCTTGG
BsiHKAI 258 GTGCAC G[AT]GC[AT]^C
650 GTGCTC
Bsp1286I 258 GTGCAC G[AGT]GC[ACT]^C
650 GTGCTC
BstYI 3 GGATCC [GA]^GATC[CT]
426 GGATCT
HincII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HinfI 148 GATTC G^A[ACGT]TC
542 GACTC
HphI 373 GGTGA GGTGA(8/7)
110 TCACC
MaeIII 38 GTCAC ^GT[ACGT]AC
109 GTCAC
MflI 3 GGATCC [GA]^GATC[CT]
426 GGATCT
PvuII 514 CAGCTG CAG^CTG
778 CAGCTG
SmlI 364 CTCAAG C^T[CT][GA]AG
544 CTCAAG
TaqI 416 TCGA T^CGA
909 TCGA
Tsp45I 38 GTCAC ^GT[GC]AC
109 GTCAC
TthHB8I 416 TCGA T^CGA
909 TCGA
XhoII 3 GGATCC [GA]^GATC[CT]
426 GGATCT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI AvaII BalI BbvI
BsrI BssKI BstF5I BstNI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MluNI
MnlI MscI MspA1I MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI TseI Tsp509I TspRI