Restriction map for
pFN21AE4315
(Linear) (Six-base) MAPSORT of: KIEE4315.seq Check: 2166 from: 1 to: 969
KIEE4315.orf
With 182 enzymes: SgfI *
March 22, 2012 17:42 ..
ApaLI G'TGCA_C
Cuts at: 0 258 969
Size: 258 711
ApoI r'AATT_y
Cuts at: 0 853 969
Size: 853 116
BamHI G'GATC_C
Cuts at: 0 3 969
Size: 3 966
BanI G'GyrC_C
Cuts at: 0 64 969
Size: 64 905
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 969
Size: 236 733
Bme1580I G_kGCm'C
Cuts at: 0 262 969
Size: 262 707
BmrI ACTGGGnnnn_n'
Cuts at: 0 691 709 969
Size: 691 18 260
Fragments arranged by size:
691 260 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 538 969
Size: 62 476 431
Fragments arranged by size:
476 431 62
Bpu10I CC'TnA_GC
Cuts at: 0 729 969
Size: 729 240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 529 969
Size: 349 180 440
Fragments arranged by size:
440 349 180
BsaHI Gr'CG_yC
Cuts at: 0 379 969
Size: 379 590
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 969
Size: 676 30 263
Fragments arranged by size:
676 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 969
Size: 187 782
BseYI C'CCAG_C
Cuts at: 0 753 969
Size: 753 216
BsiHKAI G_wGCw'C
Cuts at: 0 262 654 969
Size: 262 392 315
Fragments arranged by size:
392 315 262
BsmBI CGTCTCn'nnnn_
Cuts at: 0 370 969
Size: 370 599
Bsp1286I G_dGCh'C
Cuts at: 0 262 654 969
Size: 262 392 315
Fragments arranged by size:
392 315 262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 168 969
Size: 168 801
BstEII G'GTnAC_C
Cuts at: 0 108 969
Size: 108 861
BstYI r'GATC_y
Cuts at: 0 3 426 969
Size: 3 423 543
Fragments arranged by size:
543 423 3
BtsI GCAGTG_nn'
Cuts at: 0 222 969
Size: 222 747
ClaI AT'CG_AT
Cuts at: 0 909 969
Size: 909 60
EaeI y'GGCC_r
Cuts at: 0 188 476 803 969
Size: 188 288 327 166
Fragments arranged by size:
327 288 188 166
EarI CTCTTCn'nnn_
Cuts at: 0 217 969
Size: 217 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 969
Size: 127 842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 538 969
Size: 62 476 431
Fragments arranged by size:
476 431 62
EcoO109I rG'GnC_Cy
Cuts at: 0 711 969
Size: 711 258
EcoRI G'AATT_C
Cuts at: 0 853 969
Size: 853 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 676 708 824 856 902 934
Size: 176 32 468 32 116 32 46 32
Cuts at: 934 969
Size: 35
Fragments arranged by size:
468 176 116 46 35 32 32 32
32
HincII GTy'rAC
Cuts at: 0 333 863 969
Size: 333 530 106
Fragments arranged by size:
530 333 106
HindIII A'AGCT_T
Cuts at: 0 250 969
Size: 250 719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 309 969
Size: 162 147 660
Fragments arranged by size:
660 162 147
MscI TGG'CCA
Cuts at: 0 190 478 805 969
Size: 190 288 327 164
Fragments arranged by size:
327 288 190 164
MslI CAynn'nnrTG
Cuts at: 0 490 969
Size: 490 479
MspA1I CmG'CkG
Cuts at: 0 516 780 825 969
Size: 516 264 45 144
Fragments arranged by size:
516 264 144 45
PpuMI rG'GwC_Cy
Cuts at: 0 711 969
Size: 711 258
PstI C_TGCA'G
Cuts at: 0 785 969
Size: 785 184
PvuII CAG'CTG
Cuts at: 0 516 780 969
Size: 516 264 189
Fragments arranged by size:
516 264 189
SexAI A'CCwGG_T
Cuts at: 0 566 969
Size: 566 403
SfcI C'TryA_G
Cuts at: 0 101 507 781 969
Size: 101 406 274 188
Fragments arranged by size:
406 274 188 101
SmlI C'TyrA_G
Cuts at: 0 364 544 969
Size: 364 180 425
Fragments arranged by size:
425 364 180
SspI AAT'ATT
Cuts at: 0 413 969
Size: 413 556
StyI C'CwwG_G
Cuts at: 0 293 969
Size: 293 676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 669 969
Size: 669 300
TatI w'GTAC_w
Cuts at: 0 548 969
Size: 548 421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 969
Size: 266 32 671
Fragments arranged by size:
671 266 32
XbaI T'CTAG_A
Cuts at: 0 885 969
Size: 885 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 784 969
Size: 784 185
Enzymes that do cut:
ApaLI ApoI BamHI BanI BbsI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspMI BstEII BstYI BtsI ClaI EaeI EarI
EciI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI
MscI MslI MspA1I PpuMI PstI PvuII SexAI SfcI
SmlI SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
AscI AseI AsiSI AvaI AvrII BaeI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XhoI XmaI XmnI ZraI