################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 284 GGTACC G^GTACC AccI 577 GTAGAC GT^[AC][GT]AC Asp718I 284 GGTACC G^GTACC AspHI 143 GAGCTC G[AT]GC[AT]^C BamHI 14 GGATCC G^GATCC BseRI 565 CTCCTC GAGGAG(10/8) BsgI 701 GTGCAG GTGCAG(16/14) BsiHKAI 143 GAGCTC G[AT]GC[AT]^C BsmFI 137 GTCCC GGGAC(10/14) BspMI 421 GCAGGT ACCTGC(4/8) BstEII 529 GGTAACC G^GT[ACGT]ACC BstPI 529 GGTAACC G^GT[ACGT]ACC EcoO65I 529 GGTAACC G^GT[ACGT]ACC EcoRI 445 GAATTC G^AATTC EcoT14I 218 CCAAGG C^C[AT][AT]GG EcoT22I 731 ATGCAT ATGCA^T FauI 504 GCGGG CCCGC(4/6) HgaI 670 GACGC GACGC(5/10) KpnI 284 GGTACC GGTAC^C MseI 524 TTAA T^TAA MslI 790 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 333 CATG CATG^ NsiI 731 ATGCAT ATGCA^T PleI 439 GAGTC GAGTC(4/5) PstI 620 CTGCAG CTGCA^G SacI 143 GAGCTC GAGCT^C StyI 218 CCAAGG C^C[AT][AT]GG TaqI 861 TCGA T^CGA Tru9I 524 TTAA T^TAA Tsp45I 329 GTGAC ^GT[GC]AC TthHB8I 861 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### AcsI 445 GAATTC [GA]^AATT[CT] 465 GAATTT AcyI 265 GGCGCC G[GA]^CG[CT]C 371 GGCGCC AfaI 285 GTAC GT^AC 536 GTAC AocI 301 CCTGAGG CC^T[ACGT]AGG 812 CCTCAGG ApaI 213 GGGCCC GGGCC^C 311 GGGCCC ApoI 445 GAATTC [GA]^AATT[CT] 465 GAATTT BbeI 265 GGCGCC GGCGC^C 371 GGCGCC BbsI 63 GAAGAC GAAGAC(2/6) 517 GAAGAC BcnI 139 CCCGG CC^[GC]GG 246 CCGGG BpmI 146 CTCCAG CTGGAG(16/14) 484 CTCCAG BpuAI 63 GAAGAC GAAGAC(2/6) 517 GAAGAC BsaHI 265 GGCGCC G[GA]^CG[CT]C 371 GGCGCC BsrBI 399 CCGCTC CCGCTC(-3/-3) 276 GAGCGG BstXI 95 CCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 789 CCACAAATATGG BstYI 3 GGATCT [GA]^GATC[CT] 14 GGATCC Bsu36I 301 CCTGAGG CC^T[ACGT]AGG 812 CCTCAGG CfoI 266 GCGC GCG^C 372 GCGC DsaI 714 CCACGG C^C[GA][CT]GG 819 CCGTGG Eco81I 301 CCTGAGG CC^T[ACGT]AGG 812 CCTCAGG HaeII 265 GGCGCC [GA]GCGC^[CT] 371 GGCGCC HapII 140 CCGG C^CGG 246 CCGG HhaI 266 GCGC GCG^C 372 GCGC Hin1I 265 GGCGCC G[GA]^CG[CT]C 371 GGCGCC HinP1I 266 GCGC G^CGC 372 GCGC HpaII 140 CCGG C^CGG 246 CCGG HphI 223 GGTGA GGTGA(8/7) 571 GGTGA KasI 265 GGCGCC G^GCGCC 371 GGCGCC MflI 3 GGATCT [GA]^GATC[CT] 14 GGATCC MspA1I 81 CAGCTG C[AC]G^C[GT]G 589 CAGCTG MspI 140 CCGG C^CGG 246 CCGG NarI 265 GGCGCC GG^CGCC 371 GGCGCC NciI 139 CCCGG CC^[GC]GG 246 CCGGG PpuMI 90 AGGACCC [GA]G^G[AT]CC[CT] 177 AGGTCCT PvuII 81 CAGCTG CAG^CTG 589 CAGCTG RsaI 285 GTAC GT^AC 536 GTAC SfaNI 855 GATGC GCATC(5/9) 733 GCATC SfcI 414 CTACAG C^T[GA][CT]AG 620 CTGCAG SfoI 265 GGCGCC GGC^GCC 371 GGCGCC SmlI 182 CTCAAG C^T[CT][GA]AG 273 CTTGAG TfiI 628 GATTC G^A[AT]TC 682 GATTC Tsp509I 446 AATT ^AATT 466 AATT XhoII 3 GGATCT [GA]^GATC[CT] 14 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI TspRI