(Circular) (Six-base) MAPSORT of: pFN21AE6220.seq Check: 138 from: 1 to: 5569 pFN21AE6220 5569 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 2912 1937 Size: 646 329 4594 Fragments arranged by size: 4594 646 329 Acc65I G'GTAC_C Cuts at: 2289 2890 2289 Size: 601 4968 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 3632 5016 5284 1632 Size: 455 1545 1384 268 1917 Fragments arranged by size: 1917 1545 1384 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 4468 5445 1203 Size: 3265 977 1327 Fragments arranged by size: 3265 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AlwNI CAG_nnn'CTG Cuts at: 2247 2703 2780 3312 4884 2247 Size: 456 77 532 1572 2932 Fragments arranged by size: 2932 1572 532 456 77 ApaI G_GGCC'C Cuts at: 2222 2320 2222 Size: 98 5471 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2450 2470 2878 3104 3256 5365 1493 Size: 957 20 408 226 152 2109 1697 Fragments arranged by size: 2109 1697 957 408 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2894 1870 Size: 73 951 4545 Fragments arranged by size: 4545 951 73 BamHI G'GATC_C Cuts at: 2019 2899 3266 2019 Size: 880 367 4322 Fragments arranged by size: 4322 880 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 2289 2376 2723 2890 Size: 325 217 627 483 19 87 347 167 Cuts at: 2890 4345 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 483 347 325 217 167 87 19 BanII G_rGCy'C Cuts at: 1391 2152 2179 2222 2320 2888 1391 Size: 761 27 43 98 568 4072 Fragments arranged by size: 4072 761 568 98 43 27 BbeI G_GCGC'C Cuts at: 2274 2380 2274 Size: 106 5463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 2529 928 Size: 971 176 454 3968 Fragments arranged by size: 3968 971 454 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3760 3794 1903 Size: 34 1823 34 3678 Fragments arranged by size: 3678 1823 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 1548 Size: 5569 BfrBI ATG'CAT Cuts at: 2738 2738 Size: 5569 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4180 136 Size: 107 122 71 631 605 2508 1525 Fragments arranged by size: 2508 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2986 2986 Size: 5569 Bme1580I G_kGCm'C Cuts at: 1790 2222 2320 2624 3630 4786 1790 Size: 432 98 304 1006 1156 2573 Fragments arranged by size: 2573 1156 1006 432 304 98 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 2318 2509 Size: 416 53 83 399 24 713 188 191 Cuts at: 2509 2612 2821 3300 4258 442 Size: 103 209 479 958 1753 Fragments arranged by size: 1753 958 713 479 416 399 209 191 188 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2474 4229 1239 Size: 897 338 1755 2579 Fragments arranged by size: 2579 1755 897 338 Bpu10I CC'TnA_GC Cuts at: 2639 2639 Size: 5569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2172 2299 3694 4559 4857 2172 Size: 127 1395 865 298 2884 Fragments arranged by size: 2884 1395 865 298 127 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4232 882 Size: 617 2733 2219 Fragments arranged by size: 2733 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 5563 5563 Size: 5569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 2377 3376 3758 Size: 53 83 186 936 738 106 999 382 Cuts at: 3758 4393 275 Size: 635 1451 Fragments arranged by size: 1451 999 936 738 635 382 186 106 83 53 BsaWI w'CCGG_w Cuts at: 4002 4400 4674 4821 5232 4002 Size: 398 274 147 411 4339 Fragments arranged by size: 4339 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5344 Fragments arranged by size: 5344 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2561 1186 Size: 1375 4194 BseYI C'CCAG_C Cuts at: 2084 2458 4772 5268 2084 Size: 374 2314 496 2385 Fragments arranged by size: 2385 2314 496 374 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 1163 Size: 573 991 4005 Fragments arranged by size: 4005 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 3630 3715 4786 2152 Size: 1478 85 1071 2935 Fragments arranged by size: 2935 1478 1071 85 BsmI GAATG_Cn' Cuts at: 3080 3173 3080 Size: 93 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2179 2222 2320 2624 2888 3630 Size: 399 362 27 43 98 304 264 742 Cuts at: 3630 3715 4786 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 362 304 264 98 85 43 27 BspEI T'CCGG_A Cuts at: 5232 5232 Size: 5569 BspHI T'CATG_A Cuts at: 3458 5188 3458 Size: 1730 3839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2417 2924 844 Size: 1573 507 3489 Fragments arranged by size: 3489 1573 507 BsrBI CCG'CTC Cuts at: 1394 1823 2283 2406 3456 1394 Size: 429 460 123 1050 3507 Fragments arranged by size: 3507 1050 460 429 123 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4213 4400 1700 Size: 110 2403 187 2869 Fragments arranged by size: 2869 2403 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5569 BssSI C'ACGA_G Cuts at: 3629 4641 3629 Size: 1012 4557 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 2534 1184 Size: 1350 4219 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2801 2107 Size: 694 4875 BstYI r'GATC_y Cuts at: 1972 2008 2019 2899 3266 3651 3668 5120 5206 Size: 36 11 880 367 385 17 1452 86 Cuts at: 5206 5218 5564 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 880 385 367 346 86 36 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2307 2818 2307 Size: 511 5058 BtgI C'CryG_G Cuts at: 513 1065 2004 2719 2824 513 Size: 552 939 715 105 3258 Fragments arranged by size: 3258 939 715 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4830 Fragments arranged by size: 4830 683 56 BtsI GCAGTG_nn' Cuts at: 2622 2715 2781 3085 3879 3899 2622 Size: 93 66 304 794 20 4292 Fragments arranged by size: 4292 794 304 93 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3016 3905 5534 Size: 54 1425 50 275 18 1186 889 1629 Cuts at: 5534 8 Size: 43 Fragments arranged by size: 1629 1425 1186 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3499 1353 Size: 2146 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2555 4156 4530 4676 387 Size: 1064 138 966 1601 374 146 1280 Fragments arranged by size: 1601 1280 1064 966 374 146 138 EcoICRI GAG'CTC Cuts at: 2150 2150 Size: 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2136 2474 3632 4229 5016 5284 Size: 393 455 49 338 1158 597 787 268 Cuts at: 5284 1239 Size: 1524 Fragments arranged by size: 1524 1158 787 597 455 393 338 268 49 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2576 2845 2306 Size: 270 269 5030 Fragments arranged by size: 5030 270 269 EcoO109I rG'GnC_Cy Cuts at: 2054 2096 2183 2219 2237 2316 2317 2658 2054 Size: 42 87 36 18 79 1 341 4965 Fragments arranged by size: 4965 341 87 79 42 36 18 1 EcoRI G'AATT_C Cuts at: 2450 2878 3256 2450 Size: 428 378 4763 Fragments arranged by size: 4763 428 378 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2274 2380 4716 1678 Size: 596 106 2336 2531 Fragments arranged by size: 2531 2336 596 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2742 2774 2801 2833 4210 Size: 32 135 32 1170 32 27 32 1377 Cuts at: 4210 4242 4284 4316 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1377 1170 135 42 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KasI G'GCGC_C Cuts at: 2270 2376 2270 Size: 106 5463 KpnI G_GTAC'C Cuts at: 2293 2894 2293 Size: 601 4968 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4683 4867 1305 Size: 3378 184 2007 Fragments arranged by size: 3378 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3747 Fragments arranged by size: 3747 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 3527 3886 4045 518 Size: 648 1633 728 359 159 2042 Fragments arranged by size: 2042 1633 728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2088 2596 2985 3662 4810 5055 1606 Size: 482 508 389 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 508 482 389 245 NarI GG'CG_CC Cuts at: 2271 2377 2271 Size: 106 5463 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4078 Fragments arranged by size: 4078 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NsiI A_TGCA'T Cuts at: 2740 2740 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2927 4472 1132 Size: 97 1698 1545 2229 Fragments arranged by size: 2229 1698 1545 97 PasI CC'CwG_GG Cuts at: 1348 2100 1348 Size: 752 4817 PciI A'CATG_T Cuts at: 4468 4468 Size: 5569 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5569 PfoI T'CCnGG_A Cuts at: 2143 2152 2143 Size: 9 5560 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 5151 5183 3638 Size: 32 1481 32 4024 Fragments arranged by size: 4024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2183 2096 Size: 87 5482 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PspOMI G'GGCC_C Cuts at: 2218 2316 2218 Size: 98 5471 PstI C_TGCA'G Cuts at: 830 2629 2921 830 Size: 1799 292 3478 Fragments arranged by size: 3478 1799 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2088 2596 2088 Size: 508 5061 SacI G_AGCT'C Cuts at: 2152 2152 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2419 2625 2917 4052 4733 4924 826 Size: 219 1374 206 292 1135 681 191 1471 Fragments arranged by size: 1471 1374 1135 681 292 219 206 191 SfoI GGC'GCC Cuts at: 2272 2378 2272 Size: 106 5463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2187 2278 3673 4574 4836 820 Size: 197 926 244 91 1395 901 262 1553 Fragments arranged by size: 1553 1395 926 901 262 244 197 91 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5569 StyI C'CwwG_G Cuts at: 513 1065 2004 2223 3008 513 Size: 552 939 219 785 3074 Fragments arranged by size: 3074 939 785 552 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3596 3613 3766 3951 4396 325 Size: 1255 2016 17 153 185 445 1498 Fragments arranged by size: 2016 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3815 5527 96 Size: 274 80 33 51 1443 1838 1712 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5529 5561 2000 Size: 32 3497 32 2008 Fragments arranged by size: 3497 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI