################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccI AccII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AspI AvaII AviII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeIII MamI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 487 GGTACC G^GTACC AccIII 56 TCCGGA T^CCGGA AciI 179 GCGG CCGC(-3/-1) AflII 637 CTTAAG C^TTAAG AhdI 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 559 GGATC GGATC(4/5) Asp700I 465 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 487 GGTACC G^GTACC AspEI 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 322 CTCGAG C^[CT]CG[GA]G AvrII 630 CCTAGG C^CTAGG BanI 487 GGTACC G^G[CT][GA]CC BbvI 117 GCTGC GCAGC(8/12) BfaI 631 CTAG C^TAG BfrI 637 CTTAAG C^TTAAG BglII 792 AGATCT A^GATCT BlnI 630 CCTAGG C^CTAGG BsaI 710 GAGACC GGTCTC(1/5) BsaWI 56 TCCGGA [AT]^CCGG[AT] BseAI 56 TCCGGA T^CCGGA BseRI 15 GAGGAG GAGGAG(10/8) BsgI 185 GTGCAG GTGCAG(16/14) BsoBI 322 CTCGAG C^[CT]CG[GA]G BspEI 56 TCCGGA T^CCGGA BstYI 792 AGATCT [GA]^GATC[CT] Cac8I 92 GCATGC GC[ACGT]^[ACGT]GC Eam1105I 655 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 100 GAATTC G^AATTC Fnu4HI 117 GCTGC GC^[ACGT]GC HapII 57 CCGG C^CGG HpaII 57 CCGG C^CGG ItaI 117 GCTGC GC^[ACGT]GC KpnI 487 GGTACC GGTAC^C MaeI 631 CTAG C^TAG MaeII 329 ACGT A^CGT MflI 792 AGATCT [GA]^GATC[CT] MroI 56 TCCGGA T^CCGGA MslI 612 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 57 CCGG C^CGG NcoI 668 CCATGG C^CATGG NdeI 418 CATATG CA^TATG NlaIV 487 GGTACC GG[ACGT]^[ACGT]CC NspI 92 GCATGC [GA]CATG^[CT] PaeR7I 322 CTCGAG C^TCGAG SphI 92 GCATGC GCATG^C TseI 117 GCTGC G^C[AT]GC XhoI 322 CTCGAG C^TCGAG XhoII 792 AGATCT [GA]^GATC[CT] XmnI 465 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 488 GTAC GT^AC 555 GTAC AlwNI 528 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 616 CAGATGCTG BsiYI 72 CCATTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 714 CCTGCTTTGGG BslI 72 CCATTCCACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 714 CCTGCTTTGGG BsmFI 407 GGGAC GGGAC(10/14) 536 GGGAC BsrI 87 CCAGT ACTGG(1/-1) 588 CCAGT BstF5I 450 CATCC GGATG(2/0) 576 GGATG EcoT14I 630 CCTAGG C^C[AT][AT]GG 668 CCATGG FokI 450 CATCC GGATG(9/13) 576 GGATG HphI 190 GGTGA GGTGA(8/7) 697 GGTGA RsaI 488 GTAC GT^AC 555 GTAC SmlI 322 CTCGAG C^T[CT][GA]AG 637 CTTAAG StyI 630 CCTAGG C^C[AT][AT]GG 668 CCATGG TaqI 316 TCGA T^CGA 323 TCGA TspRI 88 CAGTG CA[GC]TG[ACGT][ACGT]^ 528 CAGTG TthHB8I 316 TCGA T^CGA 323 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AcsI AluI ApoI BsaJI BsmAI BspMI BssKI BstNI DdeI DpnII DsaI EcoRII HinfI MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI SfaNI TfiI Tru9I Tsp509I