Restriction map for
pFN21AE1488
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeII MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 46 CTTAAG C^TTAAG
BalI 227 TGGCCA TGG^CCA
BanII 287 GAGCCC G[GA]GC[CT]^C
BcnI 237 CCGGG CC^[GC]GG
BfaI 532 CTAG C^TAG
BfrI 46 CTTAAG C^TTAAG
BglI 289 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsrDI 747 CATTGC GCAATG(2/0)
BsrFI 298 GCCGGC [GA]^CCGG[CT]
BstXI 651 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 481 GGATCT [GA]^GATC[CT]
Cfr10I 298 GCCGGC [GA]^CCGG[CT]
DdeI 508 CTCAG C^T[ACGT]AG
DraI 514 TTTAAA TTT^AAA
DraII 221 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 221 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 328 GCGGG CCCGC(4/6)
FseI 297 GGCCGGCC GGCCGG^CC
KspI 326 CCGCGG CCGC^GG
MaeI 532 CTAG C^TAG
MflI 481 GGATCT [GA]^GATC[CT]
MluNI 227 TGGCCA TGG^CCA
MscI 227 TGGCCA TGG^CCA
MslI 183 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 298 GCCGGC GCC^GGC
NciI 237 CCGGG CC^[GC]GG
NgoMIV 298 GCCGGC G^CCGGC
PpuMI 221 GGGACCT [GA]G^G[AT]CC[CT]
PstI 247 CTGCAG CTGCA^G
SacII 326 CCGCGG CCGC^GG
SexAI 200 ACCAGGT A^CC[AT]GGT
SfcI 247 CTGCAG C^T[GA][CT]AG
SspI 552 AATATT AAT^ATT
TaqI 690 TCGA T^CGA
TthHB8I 690 TCGA T^CGA
XcmI 293 CCAAGGCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 481 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 261 CGCG CG^CG
327 CGCG
AlwI 119 GATCC GGATC(4/5)
481 GGATC
BmyI 287 GAGCCC G[AGT]GC[ACT]^C
527 GTGCCC
Bsp1286I 287 GAGCCC G[AGT]GC[ACT]^C
527 GTGCCC
BstUI 261 CGCG CG^CG
327 CGCG
DpnII 119 GATC ^GATC
482 GATC
EaeI 206 TGGCCG [CT]^GGCC[GA]
227 TGGCCA
HapII 237 CCGG C^CGG
299 CCGG
HinfI 7 GACTC G^A[ACGT]TC
672 GAGTC
HpaII 237 CCGG C^CGG
299 CCGG
HphI 678 GGTGA GGTGA(8/7)
323 TCACC
MboI 119 GATC ^GATC
482 GATC
MseI 47 TTAA T^TAA
515 TTAA
MspI 237 CCGG C^CGG
299 CCGG
MvnI 261 CGCG CG^CG
327 CGCG
NcoI 499 CCATGG C^CATGG
620 CCATGG
NdeII 119 GATC ^GATC
482 GATC
PleI 7 GACTC GAGTC(4/5)
672 GAGTC
PvuII 317 CAGCTG CAG^CTG
740 CAGCTG
Sau3AI 119 GATC ^GATC
482 GATC
SmlI 46 CTTAAG C^T[CT][GA]AG
428 CTCAAG
Tru9I 47 TTAA T^TAA
515 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AvaII BbvI BpmI
BsaJI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII ItaI MaeIII MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TseI Tsp45I
Tsp509I TspRI