Restriction map for
pFN21AE5603
(Circular) (Six-base) MAPSORT of: pFN21AE5603.seq Check: 3394 from: 1 to: 6121
pFN21AE5603 6121 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2300 3464 1937
Size: 363 1164 4594
Fragments arranged by size:
4594 1164 363
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 2715 4184 5568 5836 1632
Size: 795 288 1469 1384 268 1917
Fragments arranged by size:
1917 1469 1384 795 288 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 2782 5020 5997 1203
Size: 1579 2238 977 1327
Fragments arranged by size:
2238 1579 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 3864 5436 3864
Size: 1572 4549
ApaLI G'TGCA_C
Cuts at: 2864 4178 5334 2864
Size: 1314 1156 3651
Fragments arranged by size:
3651 1314 1156
ApoI r'AATT_y
Cuts at: 1493 2429 2495 3430 3656 3808 5917 1493
Size: 936 66 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 936 935 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3233 3446 1870
Size: 73 1290 213 4545
Fragments arranged by size:
4545 1290 213 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2238 2271 2238
Size: 33 6088
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3442 4897 618
Size: 325 217 627 1655 1455 1842
Fragments arranged by size:
1842 1655 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2981 3440 1391
Size: 1590 459 4072
Fragments arranged by size:
4072 1590 459
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2158 2703 928
Size: 971 259 545 4346
Fragments arranged by size:
4346 971 545 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3001 3035 4312 4346 1903
Size: 34 50 34 980 34 1277 34 3678
Fragments arranged by size:
3678 1277 980 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 2872 1548
Size: 1324 4797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2868 4182 5338 1790
Size: 1078 1314 1156 2573
Fragments arranged by size:
2573 1314 1156 1078
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3852 4810 442
Size: 416 53 83 399 24 2435 958 1753
Fragments arranged by size:
2435 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 3104 3259 2068
Size: 1036 155 4930
Fragments arranged by size:
4930 1036 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2238 2499 4781 1239
Size: 824 175 261 2282 2579
Fragments arranged by size:
2579 2282 824 261 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3153 4784 882
Size: 617 1654 1631 2219
Fragments arranged by size:
2219 1654 1631 617
BsaAI yAC'GTr
Cuts at: 493 1204 2304 3260 3912 493
Size: 711 1100 956 652 2702
Fragments arranged by size:
2702 1100 956 711 652
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 1178
Size: 30 165 30 808 30 5058
Fragments arranged by size:
5058 808 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 1186
Size: 1218 4903
BseYI C'CCAG_C
Cuts at: 2146 5324 5820 2146
Size: 3178 496 2447
Fragments arranged by size:
3178 2447 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2868 4182 4267 5338 2868
Size: 1314 85 1071 3651
Fragments arranged by size:
3651 1314 1071 85
BsmI GAATG_Cn'
Cuts at: 3004 3055 3632 3725 3004
Size: 51 577 93 5400
Fragments arranged by size:
5400 577 93 51
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2868 2981 3440 4182 4267 5338 1391
Size: 399 1078 113 459 742 85 1071 2174
Fragments arranged by size:
2174 1078 1071 742 459 399 113 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3476 844
Size: 2632 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4616 4790 65
Size: 1183 3368 174 1396
Fragments arranged by size:
3368 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3363 96
Size: 3267 2854
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 2319 4181 5193 2319
Size: 1862 1012 3247
Fragments arranged by size:
3247 1862 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6121
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 2380 1184
Size: 1196 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 2354 2354
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2212 2822 3138 3451 3818 4203 4220 5672
Size: 240 610 316 313 367 385 17 1452
Cuts at: 5672 5758 5770 6116 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 610 385 367 346 316 313
240 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2929 513
Size: 552 1864 3705
Fragments arranged by size:
3705 1864 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3147 487
Size: 683 56 1921 3461
Fragments arranged by size:
3461 1921 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2259 3637 4431 4451 2033
Size: 226 1378 794 20 3703
Fragments arranged by size:
3703 1378 794 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 2724 3425 2724
Size: 701 5420
DraIII CAC_nnn'GTG
Cuts at: 2141 2141
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 3568 4457
Size: 54 1425 50 275 18 289 1449 889
Cuts at: 4457 6086 8
Size: 1629 43
Fragments arranged by size:
1629 1449 1425 889 289 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2827 2961 4051 1353
Size: 1474 134 1090 3423
Fragments arranged by size:
3423 1474 1090 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2238 2427 2499 2715 4184 4781
Size: 393 431 175 189 72 216 1469 597
Cuts at: 4781 5568 5836 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1469 787 597 431 393 268 216
189 175 72
EcoO109I rG'GnC_Cy
Cuts at: 2732 3015 2732
Size: 283 5838
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 2024 2042 5268 1678
Size: 346 18 3226 2531
Fragments arranged by size:
3226 2531 346 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2840 2872 4762 4794 4836
Size: 32 135 32 1268 32 1890 32 42
Cuts at: 4836 4868 1373
Size: 32 2626
Fragments arranged by size:
2626 1890 1268 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3415 748
Size: 2667 3454
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3321 5235 5419 1305
Size: 2016 1914 184 2007
Fragments arranged by size:
2016 2007 1914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2121 10
Size: 54 1425 343 289 4010
Fragments arranged by size:
4010 1425 343 289 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 3276 4079 4438 4597 518
Size: 648 945 1165 803 359 159 2042
Fragments arranged by size:
2042 1165 945 803 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3537 4214 5362 5607 1606
Size: 1931 677 1148 245 2120
Fragments arranged by size:
2120 1931 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2929 513
Size: 552 1864 3705
Fragments arranged by size:
3705 1864 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 2683 2786 3479 5024 1132
Size: 97 1454 103 693 1545 2229
Fragments arranged by size:
2229 1545 1454 693 103 97
PasI CC'CwG_GG
Cuts at: 1348 2802 1348
Size: 1454 4667
PciI A'CATG_T
Cuts at: 2782 5020 2782
Size: 2238 3883
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6121
PfoI T'CCnGG_A
Cuts at: 2199 2215 2199
Size: 16 6105
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PmlI CAC'GTG
Cuts at: 3260 3260
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 3015 2732
Size: 283 5838
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 2910 3315 3691 2910
Size: 405 376 5340
Fragments arranged by size:
5340 405 376
PstI C_TGCA'G
Cuts at: 830 2028 3473 830
Size: 1198 1445 3478
Fragments arranged by size:
3478 1445 1198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SanDI GG'GwC_CC
Cuts at: 2732 2732
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2024 3388 3469 4604 5285 5476 826
Size: 219 979 1364 81 1135 681 191 1471
Fragments arranged by size:
1471 1364 1135 979 681 219 191 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2304 3912 493
Size: 1811 1608 2702
Fragments arranged by size:
2702 1811 1608
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 3386 1517
Size: 1869 4252
StyI C'CwwG_G
Cuts at: 513 1065 2420 2901 2929 2981 3560 513
Size: 552 1355 481 28 52 579 3074
Fragments arranged by size:
3074 1355 579 552 481 52 28
SwaI ATTT'AAAT
Cuts at: 2724 2724
Size: 6121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2276 4148 4165 4318 4503 4948 325
Size: 1255 696 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 696 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3363 4367 6079
Size: 274 80 33 51 1443 1386 1004 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1443 1386 1004 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 6081 6113 2127
Size: 32 3922 32 2135
Fragments arranged by size:
3922 2135 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 2710 2158
Size: 552 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 3099 4250 3099
Size: 1151 4970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI