################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MflI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 293 GCGG CCGC(-3/-1) AlwNI 725 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 35 GAGCTC G[AT]GC[AT]^C AvaI 920 CTCGGG C^[CT]CG[GA]G BalI 327 TGGCCA TGG^CCA BanII 35 GAGCTC G[GA]GC[CT]^C BbsI 849 GAAGAC GAAGAC(2/6) BbvI 234 GCAGC GCAGC(8/12) BcgI 734 GCACACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 35 GAGCTC G[AGT]GC[ACT]^C BpuAI 849 GAAGAC GAAGAC(2/6) BseRI 62 GAGGAG GAGGAG(10/8) BsiHKAI 35 GAGCTC G[AT]GC[AT]^C BsmBI 539 GAGACG CGTCTC(1/5) BsoBI 920 CTCGGG C^[CT]CG[GA]G Bsp1286I 35 GAGCTC G[AGT]GC[ACT]^C BspMI 155 ACCTGC ACCTGC(4/8) BssSI 59 CACGAG CACGAG(-5/-1) CfoI 5 GCGC GCG^C DpnII 349 GATC ^GATC EaeI 327 TGGCCA [CT]^GGCC[GA] EcoNI 441 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 382 GATATC GAT^ATC Fnu4HI 234 GCAGC GC^[ACGT]GC HaeII 4 AGCGCC [GA]GCGC^[CT] HhaI 5 GCGC GCG^C HinP1I 5 GCGC G^CGC HincII 697 GTTGAC GT[CT]^[GA]AC HindII 697 GTTGAC GT[CT]^[GA]AC ItaI 234 GCAGC GC^[ACGT]GC MaeII 542 ACGT A^CGT MboI 349 GATC ^GATC MfeI 311 CAATTG C^AATTG MluNI 327 TGGCCA TGG^CCA MscI 327 TGGCCA TGG^CCA MslI 360 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 235 CAGCTG C[AC]G^C[GT]G MunI 311 CAATTG C^AATTG MwoI 320 GCATCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 349 GATC ^GATC PpuMI 152 GGGACCT [GA]G^G[AT]CC[CT] PvuII 235 CAGCTG CAG^CTG SacI 35 GAGCTC GAGCT^C Sau3AI 349 GATC ^GATC SfcI 83 CTACAG C^T[GA][CT]AG TaqI 870 TCGA T^CGA TseI 234 GCAGC G^C[AT]GC Tsp45I 493 GTGAC ^GT[GC]AC TthHB8I 870 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsaI 296 GAGACC GGTCTC(1/5) 769 GGTCTC DraII 78 AGGCCCT [GA]G^G[ACGT]CC[CT] 152 GGGACCT EcoO109I 78 AGGCCCT [GA]G^G[ACGT]CC[CT] 152 GGGACCT HphI 492 GGTGA GGTGA(8/7) 630 GGTGA MaeIII 493 GTGAC ^GT[ACGT]AC 865 GTAAC NlaIII 331 CATG CATG^ 421 CATG PflMI 300 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 724 CCAGCTACTGG PleI 495 GACTC GAGTC(4/5) 356 GAGTC SfaNI 370 GATGC GCATC(5/9) 320 GCATC SmlI 109 CTTGAG C^T[CT][GA]AG 534 CTTGAG Van91I 300 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 724 CCAGCTACTGG XcmI 342 CCACCCTGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 478 CCAGGATTCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AluI AvaII BfaI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII EcoT14I FokI HaeIII HinfI MaeI MboII MnlI MseI MvaI NlaIV Sau96I ScrFI StyI TfiI Tru9I Tsp509I TspRI