################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 620 CCGC CCGC(-3/-1) AcsI 97 AAATTT [GA]^AATT[CT] AocI 340 CCTGAGG CC^T[ACGT]AGG ApoI 97 AAATTT [GA]^AATT[CT] BclI 368 TGATCA T^GATCA BcnI 462 CCGGG CC^[GC]GG BlpI 456 GCTGAGC GC^T[ACGT]AGC Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC BsaWI 56 TCCGGT [AT]^CCGG[AT] BspMI 569 ACCTGC ACCTGC(4/8) BsrDI 222 CATTGC GCAATG(2/0) Bst1107I 282 GTATAC GTA^TAC BstBI 406 TTCGAA TT^CGAA BstF5I 54 CATCC GGATG(2/0) BstYI 106 GGATCT [GA]^GATC[CT] BstZ17I 282 GTATAC GTA^TAC Bsu36I 340 CCTGAGG CC^T[ACGT]AGG Cac8I 226 GCCAGC GC[ACGT]^[ACGT]GC CelII 456 GCTGAGC GC^T[ACGT]AGC Cfr13I 546 GGCCC G^G[ACGT]CC DraII 545 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco81I 340 CCTGAGG CC^T[ACGT]AGG EcoO109I 545 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 619 CCCGC CCCGC(4/6) FbaI 368 TGATCA T^GATCA FokI 54 CATCC GGATG(9/13) HaeIII 546 GGCC GG^CC HincII 335 GTCGAC GT[CT]^[GA]AC HindII 335 GTCGAC GT[CT]^[GA]AC HphI 579 GGTGA GGTGA(8/7) MaeIII 417 GTTAC ^GT[ACGT]AC MflI 106 GGATCT [GA]^GATC[CT] MspA1I 74 CAGCTG C[AC]G^C[GT]G MwoI 355 GCCAAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 462 CCGGG CC^[GC]GG NcoI 426 CCATGG C^CATGG NlaIII 427 CATG CATG^ NlaIV 48 GGAGCC GG[ACGT]^[ACGT]CC NspV 406 TTCGAA TT^CGAA PflMI 53 CCATCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 511 GACTC GAGTC(4/5) PvuII 74 CAGCTG CAG^CTG SalI 335 GTCGAC G^TCGAC SapI 502 GAAGAGC GCTCTTC(1/4) Sau96I 546 GGCCC G^G[ACGT]CC SfuI 406 TTCGAA TT^CGAA TfiI 560 GAATC G^A[AT]TC Van91I 53 CCATCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 106 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 282 GTATAC GT^[AC][GT]AC 335 GTCGAC AfaI 163 GTAC GT^AC 196 GTAC AlwI 315 GATCC GGATC(4/5) 106 GGATC BanII 49 GAGCCC G[GA]GC[CT]^C 266 GAGCCC BmyI 49 GAGCCC G[AGT]GC[ACT]^C 266 GAGCCC BpmI 513 CTCCAG CTGGAG(16/14) 77 CTGGAG BsiYI 53 CCATCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 570 CCTGCCCACGG BslI 53 CCATCCGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 570 CCTGCCCACGG Bsp1286I 49 GAGCCC G[AGT]GC[ACT]^C 266 GAGCCC BstNI 477 CCTGG CC^[AT]GG 598 CCTGG DdeI 341 CTGAG C^T[ACGT]AG 457 CTGAG DsaI 426 CCATGG C^C[GA][CT]GG 575 CCACGG EarI 91 GAAGAG CTCTTC(1/4) 502 GAAGAG EcoRII 477 CCTGG ^CC[AT]GG 598 CCTGG EcoT14I 426 CCATGG C^C[AT][AT]GG 607 CCAAGG HapII 57 CCGG C^CGG 462 CCGG HindIII 16 AAGCTT A^AGCTT 632 AAGCTT HinfI 511 GACTC G^A[ACGT]TC 560 GAATC HpaII 57 CCGG C^CGG 462 CCGG Ksp632I 91 GAAGAG CTCTTC(1/4) 502 GAAGAG MseI 130 TTAA T^TAA 188 TTAA MspI 57 CCGG C^CGG 462 CCGG MvaI 477 CCTGG CC^[AT]GG 598 CCTGG PstI 71 CTGCAG CTGCA^G 110 CTGCAG RsaI 163 GTAC GT^AC 196 GTAC SfaNI 352 GATGC GCATC(5/9) 625 GATGC StyI 426 CCATGG C^C[AT][AT]GG 607 CCAAGG TaqI 336 TCGA T^CGA 407 TCGA Tru9I 130 TTAA T^TAA 188 TTAA Tsp509I 98 AATT ^AATT 273 AATT TthHB8I 336 TCGA T^CGA 407 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsaJI BssKI DpnII Fnu4HI ItaI MboI MboII MnlI NdeII Sau3AI ScrFI SfcI TseI