Restriction map for
pFN21AE4469
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII ItaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI MvnI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI TseI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AgeI 1090 ACCGGT A^CCGGT
Alw44I 1054 GTGCAC G^TGCAC
ApaLI 1054 GTGCAC G^TGCAC
AspI 1078 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 779 CTCGGG C^[CT]CG[GA]G
BalI 1227 TGGCCA TGG^CCA
BamHI 635 GGATCC G^GATCC
BanII 1005 GAGCCC G[GA]GC[CT]^C
BfaI 1237 CTAG C^TAG
BglII 1451 AGATCT A^GATCT
BpmI 57 CTGGAG CTGGAG(16/14)
BsaWI 1090 ACCGGT [AT]^CCGG[AT]
BsgI 1304 GTGCAG GTGCAG(16/14)
BsoBI 779 CTCGGG C^[CT]CG[GA]G
BspDI 790 ATCGAT AT^CGAT
BsrFI 1090 ACCGGT [GA]^CCGG[CT]
BsrI 762 ACTGG ACTGG(1/-1)
BssSI 1057 CACGAG CACGAG(-5/-1)
Cfr10I 1090 ACCGGT [GA]^CCGG[CT]
ClaI 790 ATCGAT AT^CGAT
DraI 1389 TTTAAA TTT^AAA
DraIII 485 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 631 CCATGG C^C[GA][CT]GG
EaeI 1227 TGGCCA [CT]^GGCC[GA]
EcoNI 453 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 841 GAATTC G^AATTC
FauI 1322 CCCGC CCCGC(4/6)
HincII 769 GTTAAC GT[CT]^[GA]AC
HindII 769 GTTAAC GT[CT]^[GA]AC
HpaI 769 GTTAAC GTT^AAC
MaeI 1237 CTAG C^TAG
MluNI 1227 TGGCCA TGG^CCA
MscI 1227 TGGCCA TGG^CCA
NcoI 631 CCATGG C^CATGG
PflFI 1078 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1090 ACCGGT A^CCGGT
PstI 1409 CTGCAG CTGCA^G
SgrAI 1089 CACCGGTG C[GA]^CCGG[CT]G
SphI 1013 GCATGC GCATG^C
SspI 847 AATATT AAT^ATT
TaqI 791 TCGA T^CGA
Tth111I 1078 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 791 TCGA T^CGA
XbaI 1236 TCTAGA T^CTAGA
XcmI 497 CCACTTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 1323 CCGC CCGC(-3/-1)
393 GCGG
Asp700I 332 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAAAAGATTC
AspHI 515 GTGCTC G[AT]GC[AT]^C
1054 GTGCAC
BclI 803 TGATCA T^GATCA
981 TGATCA
BsiHKAI 515 GTGCTC G[AT]GC[AT]^C
1054 GTGCAC
BsiYI 109 CCTATTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BslI 109 CCTATTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BsmFI 782 GGGAC GGGAC(10/14)
1084 GTCCC
BsrDI 344 GCAATG GCAATG(2/0)
524 GCAATG
Cac8I 1013 GCATGC GC[ACGT]^[ACGT]GC
1321 GCCCGC
FbaI 803 TGATCA T^GATCA
981 TGATCA
HaeIII 94 GGCC GG^CC
1228 GGCC
HapII 369 CCGG C^CGG
1091 CCGG
HpaII 369 CCGG C^CGG
1091 CCGG
MspI 369 CCGG C^CGG
1091 CCGG
NspI 1013 GCATGC [GA]CATG^[CT]
1031 ACATGC
PleI 926 GACTC GAGTC(4/5)
241 GAGTC
SfaNI 1045 GATGC GCATC(5/9)
757 GCATC
XmnI 332 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BmyI BsaJI BseRI BsmAI Bsp1286I
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FokI HinfI HphI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfcI StyI
TfiI Tru9I Tsp45I Tsp509I TspRI
XhoII