(Linear) (Six-base) MAPSORT of: KIEE4469.seq Check: 8475 from: 1 to: 1470 KIEE4469.orf With 182 enzymes: SgfI * March 22, 2012 18:07 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 961 1470 Size: 597 364 509 Fragments arranged by size: 597 509 364 AgeI A'CCGG_T Cuts at: 0 1090 1470 Size: 1090 380 AleI CACnn'nnGTG Cuts at: 0 1093 1470 Size: 1093 377 ApaLI G'TGCA_C Cuts at: 0 1054 1470 Size: 1054 416 ApoI r'AATT_y Cuts at: 0 396 576 841 1198 1298 1470 Size: 396 180 265 357 100 172 Fragments arranged by size: 396 357 265 180 172 100 AvaI C'yCGr_G Cuts at: 0 779 1470 Size: 779 691 BamHI G'GATC_C Cuts at: 0 635 1470 Size: 635 835 BanII G_rGCy'C Cuts at: 0 1009 1470 Size: 1009 461 BclI T'GATC_A Cuts at: 0 803 981 1470 Size: 803 178 489 Fragments arranged by size: 803 489 178 BglII A'GATC_T Cuts at: 0 1451 1470 Size: 1451 19 Bme1580I G_kGCm'C Cuts at: 0 1058 1100 1153 1470 Size: 1058 42 53 317 Fragments arranged by size: 1058 317 53 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 1470 Size: 78 1392 BsaWI w'CCGG_w Cuts at: 0 1090 1470 Size: 1090 380 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 917 947 1470 Size: 917 30 523 Fragments arranged by size: 917 523 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 92 289 429 475 1470 Size: 92 197 140 46 995 Fragments arranged by size: 995 197 140 92 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1325 1470 Size: 1325 145 BsiHKAI G_wGCw'C Cuts at: 0 519 1058 1470 Size: 519 539 412 Fragments arranged by size: 539 519 412 Bsp1286I G_dGCh'C Cuts at: 0 519 1009 1058 1100 1153 1470 Size: 519 490 49 42 53 317 Fragments arranged by size: 519 490 317 53 49 42 BsrDI GCAATG_nn' Cuts at: 0 351 531 1470 Size: 351 180 939 Fragments arranged by size: 939 351 180 BsrFI r'CCGG_y Cuts at: 0 1090 1470 Size: 1090 380 BssSI C'ACGA_G Cuts at: 0 1057 1470 Size: 1057 413 BstYI r'GATC_y Cuts at: 0 365 635 1061 1451 1470 Size: 365 270 426 390 19 Fragments arranged by size: 426 390 365 270 19 BtgI C'CryG_G Cuts at: 0 631 1470 Size: 631 839 BtsI GCAGTG_nn' Cuts at: 0 447 1112 1470 Size: 447 665 358 Fragments arranged by size: 665 447 358 ClaI AT'CG_AT Cuts at: 0 791 1470 Size: 791 679 DraI TTT'AAA Cuts at: 0 1391 1470 Size: 1391 79 DraIII CAC_nnn'GTG Cuts at: 0 490 1470 Size: 490 980 EaeI y'GGCC_r Cuts at: 0 1227 1470 Size: 1227 243 EarI CTCTTCn'nnn_ Cuts at: 0 110 282 406 968 1470 Size: 110 172 124 562 502 Fragments arranged by size: 562 502 172 124 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 78 597 961 1470 Size: 78 519 364 509 Fragments arranged by size: 519 509 364 78 EcoNI CCTnn'n_nnAGG Cuts at: 0 457 1470 Size: 457 1013 EcoRI G'AATT_C Cuts at: 0 841 1470 Size: 841 629 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 317 349 911 917 943 949 1416 1448 Size: 317 32 562 6 26 6 467 32 Cuts at: 1448 1470 Size: 22 Fragments arranged by size: 562 467 317 32 32 26 22 6 6 HincII GTy'rAC Cuts at: 0 771 1470 Size: 771 699 HpaI GTT'AAC Cuts at: 0 771 1470 Size: 771 699 MscI TGG'CCA Cuts at: 0 1229 1470 Size: 1229 241 MslI CAynn'nnrTG Cuts at: 0 223 236 589 1093 1470 Size: 223 13 353 504 377 Fragments arranged by size: 504 377 353 223 13 NcoI C'CATG_G Cuts at: 0 631 1470 Size: 631 839 NspI r_CATG'y Cuts at: 0 1017 1035 1470 Size: 1017 18 435 Fragments arranged by size: 1017 435 18 PfoI T'CCnGG_A Cuts at: 0 968 1470 Size: 968 502 PsiI TTA'TAA Cuts at: 0 566 1470 Size: 566 904 PstI C_TGCA'G Cuts at: 0 1413 1470 Size: 1413 57 SfcI C'TryA_G Cuts at: 0 69 127 869 1049 1409 1470 Size: 69 58 742 180 360 61 Fragments arranged by size: 742 360 180 69 61 58 SgrAI Cr'CCGG_yG Cuts at: 0 1090 1470 Size: 1090 380 SphI G_CATG'C Cuts at: 0 1017 1470 Size: 1017 453 SspI AAT'ATT Cuts at: 0 849 1470 Size: 849 621 StyI C'CwwG_G Cuts at: 0 476 631 1145 1470 Size: 476 155 514 325 Fragments arranged by size: 514 476 325 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 962 1470 Size: 962 508 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 90 122 1470 Size: 90 32 1348 Fragments arranged by size: 1348 90 32 Tth111I GACn'n_nGTC Cuts at: 0 1081 1470 Size: 1081 389 XbaI T'CTAG_A Cuts at: 0 1236 1470 Size: 1236 234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 504 1470 Size: 504 966 XmnI GAAnn'nnTTC Cuts at: 0 336 598 1470 Size: 336 262 872 Fragments arranged by size: 872 336 262 Enzymes that do cut: AcuI AgeI AleI ApaLI ApoI AvaI BamHI BanII BclI BglII Bme1580I BpmI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BssSI BstYI BtgI BtsI ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoRI Hin4I HincII HpaI MscI MslI NcoI NspI PfoI PsiI PstI SfcI SgrAI SphI SspI StyI TaqII TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TatI XhoI XmaI ZraI