Restriction map for
pFN21AE1572
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AocI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspLU11I BsrDI BsrFI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII Eco81I EcoO65I EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI StuI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 586 GGCGTC G[GA]^CG[CT]C
AhdI 2275 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 783 AGCGCT AGC^GCT
Asp700I 1027 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2275 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 118 GGCACC G^G[CT][GA]CC
BbsI 1421 GTCTTC GAAGAC(2/6)
BbvCI 254 CCTCAGC CCTCAGC(-5/-2)
BcgI 2238 CGAATTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 168 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1933 CTCCAG CTGGAG(16/14)
BpuAI 1421 GTCTTC GAAGAC(2/6)
BsaAI 112 TACGTG [CT]AC^GT[GA]
BsaBI 3 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 586 GGCGTC G[GA]^CG[CT]C
Bsp1407I 1043 TGTACA T^GTACA
BsrBI 40 GAGCGG CCGCTC(-3/-3)
BsrGI 1043 TGTACA T^GTACA
BssHII 246 GCGCGC G^CGCGC
BstYI 954 GGATCT [GA]^GATC[CT]
EagI 2084 CGGCCG C^GGCCG
Eam1105I 2275 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2084 CGGCCG C^GGCCG
Eco47III 783 AGCGCT AGC^GCT
Eco52I 2084 CGGCCG C^GGCCG
EcoRI 796 GAATTC G^AATTC
EcoRV 711 GATATC GAT^ATC
EcoT14I 1997 CCATGG C^C[AT][AT]GG
Hin1I 586 GGCGTC G[GA]^CG[CT]C
HindIII 2446 AAGCTT A^AGCTT
MamI 3 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1740 CAATTG C^AATTG
MflI 954 GGATCT [GA]^GATC[CT]
MunI 1740 CAATTG C^AATTG
NcoI 1997 CCATGG C^CATGG
PvuI 632 CGATCG CGAT^CG
SacI 1111 GAGCTC GAGCT^C
SalI 492 GTCGAC G^TCGAC
SapI 1113 GCTCTTC GCTCTTC(1/4)
SexAI 2278 ACCTGGT A^CC[AT]GGT
SpeI 1620 ACTAGT A^CTAGT
SphI 2172 GCATGC GCATG^C
SspBI 1043 TGTACA T^GTACA
StyI 1997 CCATGG C^C[AT][AT]GG
TfiI 1053 GATTC G^A[AT]TC
XcmI 1685 CCAAGAGACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 954 GGATCT [GA]^GATC[CT]
XmnI 1027 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 136 GTCTAC GT^[AC][GT]AC
492 GTCGAC
AlwI 822 GGATC GGATC(4/5)
954 GGATC
ApaI 338 GGGCCC GGGCC^C
672 GGGCCC
AvaI 319 CTCGAG C^[CT]CG[GA]G
1969 CTCGAG
BsaI 1689 GAGACC GGTCTC(1/5)
2120 GAGACC
BsiEI 632 CGATCG CG[GA][CT]^CG
2084 CGGCCG
BsmBI 588 CGTCTC CGTCTC(1/5)
1906 GAGACG
BsoBI 319 CTCGAG C^[CT]CG[GA]G
1969 CTCGAG
BspHI 1212 TCATGA T^CATGA
1283 TCATGA
BssSI 2062 CACGAG CACGAG(-5/-1)
2191 CACGAG
BstXI 293 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2348 CCATGCAGGTGG
DrdI 127 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1396 GACCTCCTGGTC
MaeIII 284 GTGAC ^GT[ACGT]AC
1405 GTCAC
MslI 441 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2349 CATGCAGGTG
PaeR7I 319 CTCGAG C^TCGAG
1969 CTCGAG
PflMI 2190 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2500 CCAAATGGTGG
PleI 1555 GACTC GAGTC(4/5)
1955 GAGTC
PvuII 202 CAGCTG CAG^CTG
1234 CAGCTG
RcaI 1212 TCATGA T^CATGA
1283 TCATGA
SmlI 319 CTCGAG C^T[CT][GA]AG
1969 CTCGAG
Tsp45I 284 GTGAC ^GT[GC]AC
1405 GTCAC
Van91I 2190 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2500 CCAAATGGTGG
XhoI 319 CTCGAG C^TCGAG
1969 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwNI ApaLI ApoI AspHI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I