(Circular) (Six-base) MAPSORT of: pFN21AE1572.seq Check: 9738 from: 1 to: 7285 pFN21AE1572 7285 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4348 4348 Size: 7285 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5095 278 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 2498 4628 1937 Size: 205 356 2130 4594 Fragments arranged by size: 4594 2130 356 205 Acc65I G'GTAC_C Cuts at: 4606 4606 Size: 7285 AclI AA'CG_TT Cuts at: 1563 5412 5785 1563 Size: 3849 373 3063 Fragments arranged by size: 3849 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3080 3106 5348 6732 7000 1632 Size: 1448 26 2242 1384 268 1917 Fragments arranged by size: 2242 1917 1448 1384 268 26 AfeI AGC'GCT Cuts at: 2790 2790 Size: 7285 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7088 AflIII A'CryG_T Cuts at: 1203 6184 7161 1203 Size: 4981 977 1327 Fragments arranged by size: 4981 1327 977 AgeI A'CCGG_T Cuts at: 6116 6116 Size: 7285 AhdI GACnn_n'nnGTC Cuts at: 4285 6014 4285 Size: 1729 5556 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7285 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4098 1184 Size: 2914 4371 AlwNI CAG_nnn'CTG Cuts at: 2174 2848 3103 3380 4264 4273 5028 6600 2174 Size: 674 255 277 884 9 755 1572 2859 Fragments arranged by size: 2859 1572 884 755 674 277 255 9 ApaI G_GGCC'C Cuts at: 2347 2681 2347 Size: 334 6951 ApaLI G'TGCA_C Cuts at: 2735 4064 4520 5342 6498 2735 Size: 1329 456 822 1156 3522 Fragments arranged by size: 3522 1329 1156 822 456 ApoI r'AATT_y Cuts at: 1493 2801 3035 4244 4594 4820 4972 7081 1493 Size: 1308 234 1209 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 1209 350 234 226 152 AseI AT'TA_AT Cuts at: 160 1032 5839 160 Size: 872 4807 1606 Fragments arranged by size: 4807 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 AvaI C'yCGr_G Cuts at: 1870 1943 2324 3974 4610 1870 Size: 73 381 1650 636 4545 Fragments arranged by size: 4545 1650 636 381 73 BamHI G'GATC_C Cuts at: 4615 4982 4615 Size: 367 6918 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 4606 6061 618 Size: 325 217 627 336 2483 1455 1842 Fragments arranged by size: 2483 1842 1455 627 336 325 217 BanII G_rGCy'C Cuts at: 1391 2347 2681 3013 3120 4604 1391 Size: 956 334 332 107 1484 4072 Fragments arranged by size: 4072 1484 956 334 332 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3419 928 Size: 971 1520 4794 Fragments arranged by size: 4794 1520 971 BbvCI CC'TCA_GC Cuts at: 2260 2260 Size: 7285 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4232 4266 5476 5510 1903 Size: 34 2295 34 1210 34 3678 Fragments arranged by size: 3678 2295 1210 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5177 6387 5177 Size: 1210 6075 BclI T'GATC_A Cuts at: 1548 1548 Size: 7285 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2179 5896 136 Size: 107 122 71 631 605 507 3717 1525 Fragments arranged by size: 3717 1525 631 605 507 122 107 71 BglII A'GATC_T Cuts at: 7280 7280 Size: 7285 BlpI GC'TnA_GC Cuts at: 4702 4702 Size: 7285 Bme1580I G_kGCm'C Cuts at: 1790 2126 2347 2681 2731 2739 4068 4524 5346 Size: 336 221 334 50 8 1329 456 822 Cuts at: 5346 6502 1790 Size: 1156 2573 Fragments arranged by size: 2573 1329 1156 822 456 336 334 221 50 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5016 5974 442 Size: 416 53 83 399 24 3599 958 1753 Fragments arranged by size: 3599 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7285 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3172 3172 Size: 7285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3923 5945 1239 Size: 2684 2022 2579 Fragments arranged by size: 2684 2579 2022 Bpu10I CC'TnA_GC Cuts at: 2260 2260 Size: 7285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5410 6275 6573 5410 Size: 865 298 6122 Fragments arranged by size: 6122 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3688 4119 5948 882 Size: 617 2189 431 1829 2219 Fragments arranged by size: 2219 2189 1829 617 431 BsaAI yAC'GTr Cuts at: 493 1204 2119 5076 493 Size: 711 915 2957 2702 Fragments arranged by size: 2957 2702 915 711 BsaBI GATnn'nnATC Cuts at: 2012 7279 2012 Size: 5267 2018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 5092 5474 6109 Size: 53 83 186 936 1059 2500 382 635 Cuts at: 6109 275 Size: 1451 Fragments arranged by size: 2500 1451 1059 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5718 6116 6390 6537 6948 5718 Size: 398 274 147 411 6055 Fragments arranged by size: 6055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3666 3696 1178 Size: 30 165 30 2263 30 4767 Fragments arranged by size: 4767 2263 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2639 3175 3178 3201 4289 1186 Size: 988 465 536 3 23 1088 4182 Fragments arranged by size: 4182 1088 988 536 465 23 3 BseYI C'CCAG_C Cuts at: 2177 2689 2721 4030 4556 6488 6984 2177 Size: 512 32 1309 526 1932 496 2478 Fragments arranged by size: 2478 1932 1309 526 512 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 3918 4004 4313 1163 Size: 573 762 1420 86 309 4135 Fragments arranged by size: 4135 1420 762 573 309 86 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2640 4092 4735 5496 6524 1540 Size: 275 186 639 1452 643 761 1028 2301 Fragments arranged by size: 2301 1452 1028 761 643 639 275 186 BsiHKAI G_wGCw'C Cuts at: 2739 3120 4068 4115 4524 5346 5431 6502 2739 Size: 381 948 47 409 822 85 1071 3522 Fragments arranged by size: 3522 1071 948 822 409 381 85 47 BsmI GAATG_Cn' Cuts at: 4796 4889 4796 Size: 93 7192 BsmBI CGTCTCn'nnnn_ Cuts at: 2599 3905 2599 Size: 1306 5979 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2347 2681 2731 2739 3013 3120 Size: 399 336 221 334 50 8 274 107 Cuts at: 3120 4068 4115 4524 4604 5346 5431 6502 1391 Size: 948 47 409 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 948 742 409 399 336 334 274 221 107 85 80 50 47 8 BspEI T'CCGG_A Cuts at: 6948 6948 Size: 7285 BspHI T'CATG_A Cuts at: 3217 3288 5174 6904 3217 Size: 71 1886 1730 3598 Fragments arranged by size: 3598 1886 1730 71 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4182 4230 4348 4640 844 Size: 3338 48 118 292 3489 Fragments arranged by size: 3489 3338 292 118 48 BsrBI CCG'CTC Cuts at: 1394 1823 2047 5172 1394 Size: 429 224 3125 3507 Fragments arranged by size: 3507 3125 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 5780 5954 65 Size: 1183 4532 174 1396 Fragments arranged by size: 4532 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5929 6116 1700 Size: 110 4119 187 2869 Fragments arranged by size: 4119 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3048 96 Size: 2952 4333 BssHII G'CGCG_C Cuts at: 1925 2251 1925 Size: 326 6959 BssSI C'ACGA_G Cuts at: 4067 4196 5345 6357 4067 Size: 129 1149 1012 4995 Fragments arranged by size: 4995 1149 1012 129 BstAPI GCAn_nnn'nTGC Cuts at: 794 2848 4072 4273 794 Size: 2054 1224 201 3806 Fragments arranged by size: 3806 2054 1224 201 BstBI TT'CG_AA Cuts at: 6089 6089 Size: 7285 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7285 BstXI CCAn_nnnn'nTGG Cuts at: 2305 4360 2305 Size: 2055 5230 BstYI r'GATC_y Cuts at: 1972 2959 4615 4982 5367 5384 6836 6922 6934 Size: 987 1656 367 385 17 1452 86 12 Cuts at: 6934 7280 1972 Size: 346 1977 Fragments arranged by size: 1977 1656 1452 987 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4002 4206 4215 513 Size: 552 2937 204 9 3583 Fragments arranged by size: 3583 2937 552 204 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3564 487 Size: 683 56 2338 4208 Fragments arranged by size: 4208 2338 683 56 BtsI GCAGTG_nn' Cuts at: 2193 2626 4188 4801 5595 5615 2193 Size: 433 1562 613 794 20 3863 Fragments arranged by size: 3863 1562 794 613 433 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4228 4263 529 Size: 35 3664 35 3551 Fragments arranged by size: 3664 3551 35 35 DraI TTT'AAA Cuts at: 4589 4589 Size: 7285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2138 3407 6292 809 Size: 507 822 1269 2885 1802 Fragments arranged by size: 2885 1802 1269 822 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2533 3130 3267 Size: 54 1425 50 275 18 703 597 137 Cuts at: 3267 4089 4164 4203 4374 4732 5621 7250 8 Size: 822 75 39 171 358 889 1629 43 Fragments arranged by size: 1629 1425 889 822 703 597 358 275 171 137 75 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 4089 4732 1537 Size: 275 2277 643 4090 Fragments arranged by size: 4090 2277 643 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2884 2979 3125 3219 3660 3816 3852 5215 Size: 1531 95 146 94 441 156 36 1363 Cuts at: 5215 1353 Size: 3423 Fragments arranged by size: 3423 1531 1363 441 156 146 95 94 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3871 5872 6246 6392 387 Size: 1064 138 2282 2001 374 146 1280 Fragments arranged by size: 2282 2001 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3118 3118 Size: 7285 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3080 3106 3923 5348 5945 6732 7000 Size: 393 1448 26 817 1425 597 787 268 Cuts at: 7000 1239 Size: 1524 Fragments arranged by size: 1524 1448 1425 817 787 597 393 268 26 EcoNI CCTnn'n_nnAGG Cuts at: 2156 3302 3857 2156 Size: 1146 555 5584 Fragments arranged by size: 5584 1146 555 EcoO109I rG'GnC_Cy Cuts at: 2110 2343 2398 2602 2678 3649 3876 2110 Size: 233 55 204 76 971 227 5519 Fragments arranged by size: 5519 971 233 227 204 76 55 EcoRI G'AATT_C Cuts at: 2801 4594 4972 2801 Size: 1793 378 5114 Fragments arranged by size: 5114 1793 378 EcoRV GAT'ATC Cuts at: 2718 2718 Size: 7285 FspI TGC'GCA Cuts at: 5791 5791 Size: 7285 HaeII r_GCGC'y Cuts at: 1678 2792 4014 4052 6432 1678 Size: 1114 1222 38 2380 2531 Fragments arranged by size: 2531 2380 1222 1114 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2907 2939 4271 4303 5926 Size: 32 135 32 1335 32 1332 32 1623 Cuts at: 5926 5958 6000 6032 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1623 1335 1332 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2080 2311 2499 4629 4875 669 Size: 988 281 142 231 188 2130 246 3079 Fragments arranged by size: 3079 2130 988 281 246 231 188 142 HindIII A'AGCT_T Cuts at: 748 4451 748 Size: 3703 3582 HpaI GTT'AAC Cuts at: 4875 4875 Size: 7285 KpnI G_GTAC'C Cuts at: 4610 4610 Size: 7285 MfeI C'AATT_G Cuts at: 3745 4884 3745 Size: 1139 6146 MluI A'CGCG_T Cuts at: 7161 7161 Size: 7285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2724 2805 3652 4459 6399 6583 1305 Size: 831 588 81 847 807 1940 184 2007 Fragments arranged by size: 2007 1940 847 831 807 588 184 81 MscI TGG'CCA Cuts at: 10 64 1489 1832 3269 4166 4376 10 Size: 54 1425 343 1437 897 210 2919 Fragments arranged by size: 2919 1437 1425 897 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 4358 5243 5602 5761 518 Size: 648 1284 1908 885 359 159 2042 Fragments arranged by size: 2042 1908 1284 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 3241 4088 4701 5378 6526 6771 1606 Size: 603 1032 847 613 677 1148 245 2120 Fragments arranged by size: 2120 1148 1032 847 677 613 603 245 NcoI C'CATG_G Cuts at: 513 1065 4002 513 Size: 552 2937 3796 Fragments arranged by size: 3796 2937 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7285 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7285 NotI GC'GGCC_GC Cuts at: 4732 4732 Size: 7285 NspI r_CATG'y Cuts at: 1132 1229 2317 2698 2824 4181 4643 6188 1132 Size: 97 1088 381 126 1357 462 1545 2229 Fragments arranged by size: 2229 1545 1357 1088 462 381 126 97 PasI CC'CwG_GG Cuts at: 1348 3247 1348 Size: 1899 5386 PciI A'CATG_T Cuts at: 6184 6184 Size: 7285 PflMI CCAn_nnn'nTGG Cuts at: 1108 4201 4511 1108 Size: 3093 310 3882 Fragments arranged by size: 3882 3093 310 PfoI T'CCnGG_A Cuts at: 3102 3144 3555 3102 Size: 42 411 6832 Fragments arranged by size: 6832 411 42 PmeI GTTT'AAAC Cuts at: 4589 4589 Size: 7285 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5354 5386 6867 6899 5354 Size: 32 1481 32 5740 Fragments arranged by size: 5740 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 2398 2602 3649 2110 Size: 288 204 1047 5746 Fragments arranged by size: 5746 1047 288 204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7285 PsiI TTA'TAA Cuts at: 3498 4855 3498 Size: 1357 5928 PspOMI G'GGCC_C Cuts at: 2343 2677 2343 Size: 334 6951 PspXI vC'TCGA_Gb Cuts at: 2324 2324 Size: 7285 PstI C_TGCA'G Cuts at: 830 2214 2621 3066 3520 3657 3816 3873 3987 Size: 1384 407 445 454 137 159 57 114 Cuts at: 3987 4077 4269 4637 830 Size: 90 192 368 3478 Fragments arranged by size: 3478 1384 454 445 407 368 192 159 137 114 90 57 PvuI CG_AT'CG Cuts at: 2001 2640 2001 Size: 639 6646 PvuII CAG'CTG Cuts at: 2209 3241 2209 Size: 1032 6253 SacI G_AGCT'C Cuts at: 3120 3120 Size: 7285 SalI G'TCGA_C Cuts at: 1936 2497 4627 1936 Size: 561 2130 4594 Fragments arranged by size: 4594 2130 561 SanDI GG'GwC_CC Cuts at: 2398 2398 Size: 7285 SapI GCTCTTCn'nnn_ Cuts at: 3125 3125 Size: 7285 SbfI CC_TGCA'GG Cuts at: 4637 4637 Size: 7285 ScaI AGT'ACT Cuts at: 5533 7245 5533 Size: 1712 5573 SexAI A'CCwGG_T Cuts at: 4283 4283 Size: 7285 SfcI C'TryA_G Cuts at: 826 1045 2210 2617 2873 3062 3516 3653 3812 Size: 219 1165 407 256 189 454 137 159 Cuts at: 3812 3869 3983 4073 4265 4633 5768 6449 6640 Size: 57 114 90 192 368 1135 681 191 Cuts at: 6640 826 Size: 1471 Fragments arranged by size: 1471 1165 1135 681 454 407 368 256 219 192 191 189 159 137 114 90 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7285 SmaI CCC'GGG Cuts at: 1872 4612 1872 Size: 2740 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 3974 5389 6290 6552 820 Size: 197 926 381 1650 1415 901 262 1553 Fragments arranged by size: 1650 1553 1415 926 901 381 262 197 SnaBI TAC'GTA Cuts at: 493 5076 493 Size: 4583 2702 SpeI A'CTAG_T Cuts at: 152 3625 152 Size: 3473 3812 SphI G_CATG'C Cuts at: 1132 4181 4643 1132 Size: 3049 462 3774 Fragments arranged by size: 3774 3049 462 SspI AAT'ATT Cuts at: 5 52 5209 5 Size: 47 5157 2081 Fragments arranged by size: 5157 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7285 StyI C'CwwG_G Cuts at: 513 1065 4002 4724 513 Size: 552 2937 722 3074 Fragments arranged by size: 3074 2937 722 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3520 3848 4541 5312 5329 5482 5667 Size: 1255 1940 328 693 771 17 153 185 Cuts at: 5667 6112 325 Size: 445 1498 Fragments arranged by size: 1940 1498 1255 771 693 445 328 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2565 3048 5531 Size: 274 80 33 51 1443 588 483 2483 Cuts at: 5531 7243 96 Size: 1712 138 Fragments arranged by size: 2483 1712 1443 588 483 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3614 3646 7245 7277 3614 Size: 32 3599 32 3622 Fragments arranged by size: 3622 3599 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7285 XbaI T'CTAG_A Cuts at: 4621 4621 Size: 7285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3697 3697 Size: 7285 XhoI C'TCGA_G Cuts at: 1943 2324 3974 1943 Size: 381 1650 5254 Fragments arranged by size: 5254 1650 381 XmaI C'CCGG_G Cuts at: 1870 4610 1870 Size: 2740 4545 XmnI GAAnn'nnTTC Cuts at: 3036 5414 3036 Size: 2378 4907 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5093 276 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI SrfI SwaI