(Linear) (Six-base) MAPSORT of: KIEE1572.seq Check: 2383 from: 1 to: 2580 KIEE1572.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2343 2580 Size: 2343 237 AccI GT'mk_AC Cuts at: 0 137 493 2580 Size: 137 356 2087 Fragments arranged by size: 2087 356 137 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1075 1101 2580 Size: 1075 26 1479 Fragments arranged by size: 1479 1075 26 AfeI AGC'GCT Cuts at: 0 785 2580 Size: 785 1795 AhdI GACnn_n'nnGTC Cuts at: 0 2280 2580 Size: 2280 300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2093 2580 Size: 2093 487 AlwNI CAG_nnn'CTG Cuts at: 0 169 843 1098 1375 2259 2268 2580 Size: 169 674 255 277 884 9 312 Fragments arranged by size: 884 674 312 277 255 169 9 ApaI G_GGCC'C Cuts at: 0 342 676 2580 Size: 342 334 1904 Fragments arranged by size: 1904 342 334 ApaLI G'TGCA_C Cuts at: 0 730 2059 2515 2580 Size: 730 1329 456 65 Fragments arranged by size: 1329 730 456 65 ApoI r'AATT_y Cuts at: 0 796 1030 2239 2580 Size: 796 234 1209 341 Fragments arranged by size: 1209 796 341 234 AvaI C'yCGr_G Cuts at: 0 319 1969 2580 Size: 319 1650 611 Fragments arranged by size: 1650 611 319 BanI G'GyrC_C Cuts at: 0 118 2580 Size: 118 2462 BanII G_rGCy'C Cuts at: 0 342 676 1008 1115 2580 Size: 342 334 332 107 1465 Fragments arranged by size: 1465 342 334 332 107 BbsI GAAGACnn'nnnn_ Cuts at: 0 1414 2580 Size: 1414 1166 BbvCI CC'TCA_GC Cuts at: 0 255 2580 Size: 255 2325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2227 2261 2580 Size: 2227 34 319 Fragments arranged by size: 2227 319 34 BglI GCCn_nnn'nGGC Cuts at: 0 174 2580 Size: 174 2406 Bme1580I G_kGCm'C Cuts at: 0 121 342 676 726 734 2063 2519 2580 Size: 121 221 334 50 8 1329 456 61 Fragments arranged by size: 1329 456 334 221 121 61 50 8 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1167 2580 Size: 1167 1413 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1918 2580 Size: 1918 662 Bpu10I CC'TnA_GC Cuts at: 0 255 2580 Size: 255 2325 BsaI GGTCTCn'nnnn_ Cuts at: 0 1683 2114 2580 Size: 1683 431 466 Fragments arranged by size: 1683 466 431 BsaAI yAC'GTr Cuts at: 0 114 2580 Size: 114 2466 BsaBI GATnn'nnATC Cuts at: 0 7 2580 Size: 7 2573 BsaHI Gr'CG_yC Cuts at: 0 587 2580 Size: 587 1993 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1661 1691 2580 Size: 1661 30 889 Fragments arranged by size: 1661 889 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 634 1170 1173 1196 2284 2580 Size: 169 465 536 3 23 1088 296 Fragments arranged by size: 1088 536 465 296 169 23 3 BseYI C'CCAG_C Cuts at: 0 172 684 716 2025 2551 2580 Size: 172 512 32 1309 526 29 Fragments arranged by size: 1309 526 512 172 32 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1913 1999 2308 2580 Size: 493 1420 86 309 272 Fragments arranged by size: 1420 493 309 272 86 BsiEI CG_ry'CG Cuts at: 0 635 2087 2580 Size: 635 1452 493 Fragments arranged by size: 1452 635 493 BsiHKAI G_wGCw'C Cuts at: 0 734 1115 2063 2110 2519 2580 Size: 734 381 948 47 409 61 Fragments arranged by size: 948 734 409 381 61 47 BsmBI CGTCTCn'nnnn_ Cuts at: 0 594 1900 2580 Size: 594 1306 680 Fragments arranged by size: 1306 680 594 Bsp1286I G_dGCh'C Cuts at: 0 121 342 676 726 734 1008 1115 2063 Size: 121 221 334 50 8 274 107 948 Cuts at: 2063 2110 2519 2580 Size: 47 409 61 Fragments arranged by size: 948 409 334 274 221 121 107 61 50 47 8 BspHI T'CATG_A Cuts at: 0 1212 1283 2580 Size: 1212 71 1297 Fragments arranged by size: 1297 1212 71 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2177 2225 2343 2580 Size: 2177 48 118 237 Fragments arranged by size: 2177 237 118 48 BsrBI CCG'CTC Cuts at: 0 42 2580 Size: 42 2538 BsrGI T'GTAC_A Cuts at: 0 1043 2580 Size: 1043 1537 BssHII G'CGCG_C Cuts at: 0 246 2580 Size: 246 2334 BssSI C'ACGA_G Cuts at: 0 2062 2191 2580 Size: 2062 129 389 Fragments arranged by size: 2062 389 129 BstAPI GCAn_nnn'nTGC Cuts at: 0 843 2067 2268 2580 Size: 843 1224 201 312 Fragments arranged by size: 1224 843 312 201 BstXI CCAn_nnnn'nTGG Cuts at: 0 300 2355 2580 Size: 300 2055 225 Fragments arranged by size: 2055 300 225 BstYI r'GATC_y Cuts at: 0 954 2580 Size: 954 1626 BtgI C'CryG_G Cuts at: 0 1997 2201 2210 2580 Size: 1997 204 9 370 Fragments arranged by size: 1997 370 204 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1559 2580 Size: 1559 1021 BtsI GCAGTG_nn' Cuts at: 0 188 621 2183 2580 Size: 188 433 1562 397 Fragments arranged by size: 1562 433 397 188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2223 2258 2580 Size: 2223 35 322 Fragments arranged by size: 2223 322 35 DrdI GACnn_nn'nnGTC Cuts at: 0 133 1402 2580 Size: 133 1269 1178 Fragments arranged by size: 1269 1178 133 EaeI y'GGCC_r Cuts at: 0 528 1125 1262 2084 2159 2198 2369 2580 Size: 528 597 137 822 75 39 171 211 Fragments arranged by size: 822 597 528 211 171 137 75 39 EagI C'GGCC_G Cuts at: 0 2084 2580 Size: 2084 496 EarI CTCTTCn'nnn_ Cuts at: 0 879 974 1120 1214 1655 1811 1847 2580 Size: 879 95 146 94 441 156 36 733 Fragments arranged by size: 879 733 441 156 146 95 94 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1866 2580 Size: 1866 714 EcoICRI GAG'CTC Cuts at: 0 1113 2580 Size: 1113 1467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1075 1101 1918 2580 Size: 1075 26 817 662 Fragments arranged by size: 1075 817 662 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 151 1297 1852 2580 Size: 151 1146 555 728 Fragments arranged by size: 1146 728 555 151 EcoO109I rG'GnC_Cy Cuts at: 0 105 338 393 597 673 1644 1871 2580 Size: 105 233 55 204 76 971 227 709 Fragments arranged by size: 971 709 233 227 204 105 76 55 EcoRI G'AATT_C Cuts at: 0 796 2580 Size: 796 1784 EcoRV GAT'ATC Cuts at: 0 713 2580 Size: 713 1867 HaeII r_GCGC'y Cuts at: 0 787 2009 2047 2580 Size: 787 1222 38 533 Fragments arranged by size: 1222 787 533 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 902 934 2266 2298 2580 Size: 902 32 1332 32 282 Fragments arranged by size: 1332 902 282 32 32 HincII GTy'rAC Cuts at: 0 75 306 494 2580 Size: 75 231 188 2086 Fragments arranged by size: 2086 231 188 75 HindIII A'AGCT_T Cuts at: 0 2446 2580 Size: 2446 134 MfeI C'AATT_G Cuts at: 0 1740 2580 Size: 1740 840 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 131 719 800 1647 2454 2580 Size: 131 588 81 847 807 126 Fragments arranged by size: 847 807 588 131 126 81 MscI TGG'CCA Cuts at: 0 1264 2161 2371 2580 Size: 1264 897 210 209 Fragments arranged by size: 1264 897 210 209 MslI CAynn'nnrTG Cuts at: 0 445 2353 2580 Size: 445 1908 227 Fragments arranged by size: 1908 445 227 MspA1I CmG'CkG Cuts at: 0 204 1236 2083 2580 Size: 204 1032 847 497 Fragments arranged by size: 1032 847 497 204 NcoI C'CATG_G Cuts at: 0 1997 2580 Size: 1997 583 NspI r_CATG'y Cuts at: 0 312 693 819 2176 2580 Size: 312 381 126 1357 404 Fragments arranged by size: 1357 404 381 312 126 PasI CC'CwG_GG Cuts at: 0 1242 2580 Size: 1242 1338 PflMI CCAn_nnn'nTGG Cuts at: 0 2196 2506 2580 Size: 2196 310 74 Fragments arranged by size: 2196 310 74 PfoI T'CCnGG_A Cuts at: 0 1097 1139 1550 2580 Size: 1097 42 411 1030 Fragments arranged by size: 1097 1030 411 42 PpuMI rG'GwC_Cy Cuts at: 0 105 393 597 1644 2580 Size: 105 288 204 1047 936 Fragments arranged by size: 1047 936 288 204 105 PsiI TTA'TAA Cuts at: 0 1493 2580 Size: 1493 1087 PspOMI G'GGCC_C Cuts at: 0 338 672 2580 Size: 338 334 1908 Fragments arranged by size: 1908 338 334 PspXI vC'TCGA_Gb Cuts at: 0 319 2580 Size: 319 2261 PstI C_TGCA'G Cuts at: 0 209 616 1061 1515 1652 1811 1868 1982 Size: 209 407 445 454 137 159 57 114 Cuts at: 1982 2072 2264 2580 Size: 90 192 316 Fragments arranged by size: 454 445 407 316 209 192 159 137 114 90 57 PvuI CG_AT'CG Cuts at: 0 635 2580 Size: 635 1945 PvuII CAG'CTG Cuts at: 0 204 1236 2580 Size: 204 1032 1344 Fragments arranged by size: 1344 1032 204 SacI G_AGCT'C Cuts at: 0 1115 2580 Size: 1115 1465 SalI G'TCGA_C Cuts at: 0 492 2580 Size: 492 2088 SanDI GG'GwC_CC Cuts at: 0 393 2580 Size: 393 2187 SapI GCTCTTCn'nnn_ Cuts at: 0 1120 2580 Size: 1120 1460 SexAI A'CCwGG_T Cuts at: 0 2278 2580 Size: 2278 302 SfcI C'TryA_G Cuts at: 0 205 612 868 1057 1511 1648 1807 1864 Size: 205 407 256 189 454 137 159 57 Cuts at: 1864 1978 2068 2260 2580 Size: 114 90 192 320 Fragments arranged by size: 454 407 320 256 205 192 189 159 137 114 90 57 SmlI C'TyrA_G Cuts at: 0 319 1969 2580 Size: 319 1650 611 Fragments arranged by size: 1650 611 319 SpeI A'CTAG_T Cuts at: 0 1620 2580 Size: 1620 960 SphI G_CATG'C Cuts at: 0 2176 2580 Size: 2176 404 StyI C'CwwG_G Cuts at: 0 1997 2580 Size: 1997 583 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1515 1843 2536 2580 Size: 1515 328 693 44 Fragments arranged by size: 1515 693 328 44 TatI w'GTAC_w Cuts at: 0 560 1043 2580 Size: 560 483 1537 Fragments arranged by size: 1537 560 483 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1609 1641 2580 Size: 1609 32 939 Fragments arranged by size: 1609 939 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1692 2580 Size: 1692 888 XhoI C'TCGA_G Cuts at: 0 319 1969 2580 Size: 319 1650 611 Fragments arranged by size: 1650 611 319 XmnI GAAnn'nnTTC Cuts at: 0 1031 2580 Size: 1031 1549 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SexAI SfcI SmlI SpeI SphI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AleI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaWI BsiWI BsmI BspEI BsrDI BsrFI BstBI BstEII BstZ17I Bsu36I ClaI DraI DraIII FalI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsrI RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI