Restriction map for
pFN21AE4546
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccIII AclI AflII Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspI AvaI AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI
Bsu36I ClaI CpoI DraIII DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
HpaI KasI KpnI KspI MluI
MroI MslI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 122 GACGTC GACGT^C
AcyI 122 GACGTC G[GA]^CG[CT]C
AflIII 1790 ACATGT A^C[GA][CT]GT
AgeI 1502 ACCGGT A^CCGGT
AhdI 385 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 85 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1683 ATTAAT AT^TAAT
AsnI 1683 ATTAAT AT^TAAT
Asp700I 313 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 385 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1863 GTGCTC G[AT]GC[AT]^C
AviII 261 TGCGCA TGC^GCA
BanI 204 GGCACC G^G[CT][GA]CC
BbvI 1558 GCTGC GCAGC(8/12)
BglI 196 GCCAGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1094 CACGTA [CT]AC^GT[GA]
BsaHI 122 GACGTC G[GA]^CG[CT]C
BsaI 146 GAGACC GGTCTC(1/5)
BsaWI 1502 ACCGGT [AT]^CCGG[AT]
BsgI 1666 GTGCAG GTGCAG(16/14)
BsiHKAI 1863 GTGCTC G[AT]GC[AT]^C
BspLU11I 1790 ACATGT A^CATGT
BspMI 243 ACCTGC ACCTGC(4/8)
Bst1107I 969 GTATAC GTA^TAC
BstAPI 18 GCATGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 893 CCAAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 969 GTATAC GTA^TAC
DraI 1798 TTTAAA TTT^AAA
DraII 722 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 385 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 656 CTCTTC CTCTTC(1/4)
EcoO109I 722 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 1113 GCGGG CCCGC(4/6)
FspI 261 TGCGCA TGC^GCA
HaeII 1617 AGCGCC [GA]GCGC^[CT]
Hin1I 122 GACGTC G[GA]^CG[CT]C
HindIII 1766 AAGCTT A^AGCTT
Ksp632I 656 CTCTTC CTCTTC(1/4)
MfeI 113 CAATTG C^AATTG
MunI 113 CAATTG C^AATTG
NheI 459 GCTAGC G^CTAGC
PflMI 127 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1502 ACCGGT A^CCGGT
PpuMI 722 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1683 ATTAAT AT^TAAT
PvuII 166 CAGCTG CAG^CTG
SfaNI 298 GATGC GCATC(5/9)
SmlI 694 CTCAAG C^T[CT][GA]AG
SpeI 1164 ACTAGT A^CTAGT
TseI 1558 GCTGC G^C[AT]GC
Van91I 127 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 375 TCTAGA T^CTAGA
XcmI 893 CCAAAGTTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 313 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccI 969 GTATAC GT^[AC][GT]AC
1447 GTCTAC
AccII 544 CGCG CG^CG
1584 CGCG
BalI 194 TGGCCA TGG^CCA
222 TGGCCA
BamHI 347 GGATCC G^GATCC
1656 GGATCC
BbsI 1294 GAAGAC GAAGAC(2/6)
1315 GTCTTC
BclI 687 TGATCA T^GATCA
1343 TGATCA
BcnI 37 CCGGG CC^[GC]GG
474 CCGGG
BlpI 90 GCTGAGC GC^T[ACGT]AGC
178 GCTGAGC
Bpu1102I 90 GCTGAGC GC^T[ACGT]AGC
178 GCTGAGC
BpuAI 1294 GAAGAC GAAGAC(2/6)
1315 GTCTTC
BsaBI 1036 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1344 GATCAGTATC
BsmAI 146 GAGAC GTCTC(1/5)
1787 GAGAC
BsmFI 171 GTCCC GGGAC(10/14)
1822 GTCCC
BsrFI 149 ACCGGC [GA]^CCGG[CT]
1502 ACCGGT
BstEII 478 GGTGACC G^GT[ACGT]ACC
1498 GGTCACC
BstNI 174 CCAGG CC^[AT]GG
1660 CCAGG
BstPI 478 GGTGACC G^GT[ACGT]ACC
1498 GGTCACC
BstUI 544 CGCG CG^CG
1584 CGCG
CelII 90 GCTGAGC GC^T[ACGT]AGC
178 GCTGAGC
CfoI 262 GCGC GCG^C
1618 GCGC
Cfr10I 149 ACCGGC [GA]^CCGG[CT]
1502 ACCGGT
DrdI 715 GACAAACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1438 GACAACCCTGTC
EaeI 194 TGGCCA [CT]^GGCC[GA]
222 TGGCCA
EcoO65I 478 GGTGACC G^GT[ACGT]ACC
1498 GGTCACC
EcoRII 174 CCAGG ^CC[AT]GG
1660 CCAGG
FbaI 687 TGATCA T^GATCA
1343 TGATCA
Fnu4HI 456 GCGGC GC^[ACGT]GC
1558 GCTGC
HgaI 87 GACGC GACGC(5/10)
1585 GCGTC
HhaI 262 GCGC GCG^C
1618 GCGC
HinP1I 262 GCGC G^CGC
1618 GCGC
HincII 327 GTCAAC GT[CT]^[GA]AC
550 GTTGAC
HindII 327 GTCAAC GT[CT]^[GA]AC
550 GTTGAC
ItaI 456 GCGGC GC^[ACGT]GC
1558 GCTGC
MamI 1036 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1344 GATCAGTATC
MluNI 194 TGGCCA TGG^CCA
222 TGGCCA
MscI 194 TGGCCA TGG^CCA
222 TGGCCA
MspA1I 166 CAGCTG C[AC]G^C[GT]G
454 CAGCGG
MvaI 174 CCAGG CC^[AT]GG
1660 CCAGG
MvnI 544 CGCG CG^CG
1584 CGCG
NciI 37 CCGGG CC^[GC]GG
474 CCGGG
PleI 55 GACTC GAGTC(4/5)
1834 GACTC
SfcI 96 CTACAG C^T[GA][CT]AG
1430 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BfaI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII EcoT14I FokI HaeIII
HapII HinfI HpaII HphI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI StyI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XhoII