(Circular) (Six-base) MAPSORT of: pFN21AE4546.seq Check: 4053 from: 1 to: 6625 pFN21AE4546 6625 bp With 182 enzymes: SgfI * March 28, 2012 14:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2257 2257 Size: 6625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2131 4435 278 Size: 53 83 186 936 595 2304 2468 Fragments arranged by size: 2468 2304 936 595 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 3453 3968 1937 Size: 1038 478 515 4594 Fragments arranged by size: 4594 1038 515 478 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 3924 4752 5125 1563 Size: 2361 828 373 3063 Fragments arranged by size: 3063 2361 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3759 4688 6072 6340 1632 Size: 2127 929 1384 268 1917 Fragments arranged by size: 2127 1917 1384 929 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 3795 5524 6501 1203 Size: 2592 1729 977 1327 Fragments arranged by size: 2592 1729 1327 977 AgeI A'CCGG_T Cuts at: 3507 5456 3507 Size: 1949 4676 AhdI GACnn_n'nnGTC Cuts at: 2395 5354 2395 Size: 2959 3666 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AlwNI CAG_nnn'CTG Cuts at: 2095 4368 5940 2095 Size: 2273 1572 2780 Fragments arranged by size: 2780 2273 1572 ApaLI G'TGCA_C Cuts at: 4682 5838 4682 Size: 1156 5469 ApoI r'AATT_y Cuts at: 1493 2297 2422 2826 3756 3934 4160 4312 6421 Size: 804 125 404 930 178 226 152 2109 Cuts at: 6421 1493 Size: 1697 Fragments arranged by size: 2109 1697 930 804 404 226 178 152 125 AseI AT'TA_AT Cuts at: 160 1032 3689 5179 160 Size: 872 2657 1490 1606 Fragments arranged by size: 2657 1606 1490 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 3950 1870 Size: 73 2007 4545 Fragments arranged by size: 4545 2007 73 BamHI G'GATC_C Cuts at: 2352 3661 3955 4322 2352 Size: 1309 294 367 4655 Fragments arranged by size: 4655 1309 367 294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 3946 5401 618 Size: 325 217 627 422 1737 1455 1842 Fragments arranged by size: 1842 1737 1455 627 422 325 217 BanII G_rGCy'C Cuts at: 1391 3944 1391 Size: 2553 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3306 3313 928 Size: 971 1407 7 4240 Fragments arranged by size: 4240 1407 971 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 2692 3348 1548 Size: 1144 656 4825 Fragments arranged by size: 4825 1144 656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 5236 136 Size: 107 122 71 631 605 535 3029 1525 Fragments arranged by size: 3029 1525 631 605 535 122 107 71 BglII A'GATC_T Cuts at: 6620 6620 Size: 6625 BlpI GC'TnA_GC Cuts at: 2096 2184 4042 2096 Size: 88 1858 4679 Fragments arranged by size: 4679 1858 88 Bme1580I G_kGCm'C Cuts at: 1790 2048 2898 4686 5842 1790 Size: 258 850 1788 1156 2573 Fragments arranged by size: 2573 1788 1156 850 258 BmgBI CAC'GTC Cuts at: 1318 2049 1318 Size: 731 5894 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3459 3562 3824 Size: 416 53 83 399 24 2042 103 262 Cuts at: 3824 4356 5314 442 Size: 532 958 1753 Fragments arranged by size: 2042 1753 958 532 416 399 262 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2468 1056 Size: 1412 5213 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2174 2174 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3114 3615 5285 1239 Size: 905 970 501 1670 2579 Fragments arranged by size: 2579 1670 970 905 501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2684 4750 5615 5913 2684 Size: 2066 865 298 3396 Fragments arranged by size: 3396 2066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2145 5288 882 Size: 617 646 3143 2219 Fragments arranged by size: 3143 2219 646 617 BsaAI yAC'GTr Cuts at: 493 1204 3101 4416 493 Size: 711 1897 1315 2702 Fragments arranged by size: 2702 1897 1315 711 BsaBI GATnn'nnATC Cuts at: 3045 3353 6619 3045 Size: 308 3266 3051 Fragments arranged by size: 3266 3051 308 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2128 4432 4814 5449 Size: 53 83 186 936 595 2304 382 635 Cuts at: 5449 275 Size: 1451 Fragments arranged by size: 2304 1451 936 635 595 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3507 5058 5456 5730 5877 6288 3507 Size: 1551 398 274 147 411 3844 Fragments arranged by size: 3844 1551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6400 Fragments arranged by size: 6400 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6625 BseYI C'CCAG_C Cuts at: 5828 6324 5828 Size: 496 6129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3692 1163 Size: 573 1956 4096 Fragments arranged by size: 4096 1956 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3872 4686 4771 5842 3872 Size: 814 85 1071 4655 Fragments arranged by size: 4655 1071 814 85 BsmI GAATG_Cn' Cuts at: 4136 4229 4136 Size: 93 6532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2898 3872 3944 4686 4771 5842 Size: 399 258 850 974 72 742 85 1071 Cuts at: 5842 1391 Size: 2174 Fragments arranged by size: 2174 1071 974 850 742 399 258 85 72 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2257 3980 844 Size: 1413 1723 3489 Fragments arranged by size: 3489 1723 1413 BsrBI CCG'CTC Cuts at: 1394 1823 4512 1394 Size: 429 2689 3507 Fragments arranged by size: 3507 2689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5120 5294 65 Size: 1183 3872 174 1396 Fragments arranged by size: 3872 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2154 3507 5269 5456 1700 Size: 110 344 1353 1762 187 2869 Fragments arranged by size: 2869 1762 1353 344 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 4685 5697 4685 Size: 1012 5613 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 794 Size: 1235 5390 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 2483 3503 1184 Size: 1299 1020 4306 Fragments arranged by size: 4306 1299 1020 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 6625 BstYI r'GATC_y Cuts at: 1972 2352 2860 3661 3955 4322 4707 4724 6176 Size: 380 508 801 294 367 385 17 1452 Cuts at: 6176 6262 6274 6620 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 801 508 385 380 367 346 294 86 17 12 BstZ17I GTA'TAC Cuts at: 2976 2976 Size: 6625 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 4141 4935 4955 4141 Size: 794 20 5811 Fragments arranged by size: 5811 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3805 3929 3805 Size: 124 6501 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2726 3449 5632 809 Size: 507 1410 723 2183 1802 Fragments arranged by size: 2183 1802 1410 723 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2227 4072 Size: 54 1425 50 275 18 369 28 1845 Cuts at: 4072 4961 6590 8 Size: 889 1629 43 Fragments arranged by size: 1845 1629 1425 889 369 275 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2667 4555 1353 Size: 1314 1888 3423 Fragments arranged by size: 3423 1888 1314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2163 5212 5586 5732 387 Size: 1064 138 574 3049 374 146 1280 Fragments arranged by size: 3049 1280 1064 574 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3114 3615 3759 4688 5285 6072 Size: 393 512 970 501 144 929 597 787 Cuts at: 6072 6340 1239 Size: 268 1524 Fragments arranged by size: 1524 970 929 787 597 512 501 393 268 144 EcoO109I rG'GnC_Cy Cuts at: 2728 2728 Size: 6625 EcoRI G'AATT_C Cuts at: 3934 4312 3934 Size: 378 6247 FspI TGC'GCA Cuts at: 2268 5131 2268 Size: 2863 3762 HaeII r_GCGC'y Cuts at: 1678 3626 5772 1678 Size: 1948 2146 2531 Fragments arranged by size: 2531 2146 1948 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 3856 3888 5266 Size: 32 135 32 809 32 1443 32 1378 Cuts at: 5266 5298 5340 5372 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 1378 809 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2334 2557 3969 4215 669 Size: 988 281 396 223 1412 246 3079 Fragments arranged by size: 3079 1412 988 396 281 246 223 HindIII A'AGCT_T Cuts at: 748 3771 748 Size: 3023 3602 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 2118 4224 2118 Size: 2106 4519 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2156 2762 2946 5739 5923 1305 Size: 851 606 184 2793 184 2007 Fragments arranged by size: 2793 2007 851 606 184 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2229 10 Size: 54 1425 343 369 28 4406 Fragments arranged by size: 4406 1425 369 343 54 28 MslI CAynn'nnrTG Cuts at: 518 1166 4583 4942 5101 518 Size: 648 3417 359 159 2042 Fragments arranged by size: 3417 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 2461 4041 4718 5866 6111 1606 Size: 567 288 1580 677 1148 245 2120 Fragments arranged by size: 2120 1580 1148 677 567 288 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NheI G'CTAG_C Cuts at: 1052 2464 1052 Size: 1412 5213 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2273 2398 2770 3799 3983 5528 1132 Size: 97 1044 125 372 1029 184 1545 2229 Fragments arranged by size: 2229 1545 1044 1029 372 184 125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 3795 5524 3795 Size: 1729 4896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2138 1108 Size: 1030 5595 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 2728 Size: 6625 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 3156 4195 3156 Size: 1039 5586 PstI C_TGCA'G Cuts at: 830 3977 830 Size: 3147 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2173 2173 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 4873 6585 4873 Size: 1712 4913 SfcI C'TryA_G Cuts at: 826 1045 2101 3435 3973 5108 5789 5980 826 Size: 219 1056 1334 538 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 1056 681 538 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2699 4729 5630 5892 820 Size: 197 926 756 2030 901 262 1553 Fragments arranged by size: 2030 1553 926 901 756 262 197 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 3169 152 Size: 3017 3608 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6625 StyI C'CwwG_G Cuts at: 513 1065 2004 2593 3016 3403 3843 4064 513 Size: 552 939 589 423 387 440 221 3074 Fragments arranged by size: 3074 939 589 552 440 423 387 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4652 4669 4822 5007 5452 325 Size: 1255 3072 17 153 185 445 1498 Fragments arranged by size: 3072 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3277 4871 6583 Size: 274 80 33 51 1443 1300 1594 1712 Cuts at: 6583 96 Size: 138 Fragments arranged by size: 1712 1594 1443 1300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2997 3029 6585 6617 2997 Size: 32 3556 32 3005 Fragments arranged by size: 3556 3005 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 2380 3961 2380 Size: 1581 5044 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2905 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 2322 4754 2322 Size: 2432 4193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2129 4433 276 Size: 53 83 186 936 595 2304 2468 Fragments arranged by size: 2468 2304 936 595 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI