################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BclI BfaI BfrI BglI BglII BlnI BmyI BpmI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AlwNI 438 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 365 AGCGCT AGC^GCT BbrPI 573 CACGTG CAC^GTG BcgI 378 GCAAATCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 251 GGATAC GTATCC(6/5) BsaAI 573 CACGTG [CT]AC^GT[GA] Bsp1407I 577 TGTACA T^GTACA BspMI 374 ACCTGC ACCTGC(4/8) BsrGI 577 TGTACA T^GTACA BsrI 880 CCAGT ACTGG(1/-1) BstF5I 299 GGATG GGATG(2/0) BstYI 20 GGATCT [GA]^GATC[CT] CfoI 366 GCGC GCG^C DsaI 136 CCACGG C^C[GA][CT]GG EaeI 46 TGGCCG [CT]^GGCC[GA] Eco47III 365 AGCGCT AGC^GCT FokI 299 GGATG GGATG(9/13) HaeII 365 AGCGCT [GA]GCGC^[CT] HaeIII 47 GGCC GG^CC HgaI 307 GACGC GACGC(5/10) HhaI 366 GCGC GCG^C HinP1I 366 GCGC G^CGC HphI 663 TCACC GGTGA(8/7) MaeII 574 ACGT A^CGT MfeI 498 CAATTG C^AATTG MflI 20 GGATCT [GA]^GATC[CT] MspA1I 56 CAGCTG C[AC]G^C[GT]G MunI 498 CAATTG C^AATTG PmaCI 573 CACGTG CAC^GTG PmlI 573 CACGTG CAC^GTG PstI 847 CTGCAG CTGCA^G PvuII 56 CAGCTG CAG^CTG SapI 874 GAAGAGC GCTCTTC(1/4) SspBI 577 TGTACA T^GTACA XhoII 20 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 578 GTAC GT^AC 659 GTAC AflIII 572 ACACGT A^C[GA][CT]GT 574 ACGTGT AlwI 346 GATCC GGATC(4/5) 20 GGATC AocI 260 CCTTAGG CC^T[ACGT]AGG 550 CCTGAGG AvaII 265 GGTCC G^G[AT]CC 555 GGACC BcnI 623 CCCGG CC^[GC]GG 671 CCGGG BlpI 4 GCTCAGC GC^T[ACGT]AGC 678 GCTGAGC Bpu1102I 4 GCTCAGC GC^T[ACGT]AGC 678 GCTGAGC BsgI 635 GTGCAG GTGCAG(16/14) 904 GTGCAG BsmAI 197 GAGAC GTCTC(1/5) 314 GAGAC BsmFI 958 GGGAC GGGAC(10/14) 506 GTCCC BsrDI 153 CATTGC GCAATG(2/0) 646 GCAATG Bsu36I 260 CCTTAGG CC^T[ACGT]AGG 550 CCTGAGG CelII 4 GCTCAGC GC^T[ACGT]AGC 678 GCTGAGC Cfr13I 265 GGTCC G^G[ACGT]CC 555 GGACC EarI 517 GAAGAG CTCTTC(1/4) 874 GAAGAG Eco81I 260 CCTTAGG CC^T[ACGT]AGG 550 CCTGAGG EcoT14I 854 CCAAGG C^C[AT][AT]GG 869 CCAAGG Ksp632I 517 GAAGAG CTCTTC(1/4) 874 GAAGAG MseI 146 TTAA T^TAA 421 TTAA MslI 455 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 642 CATCGCAATG NciI 623 CCCGG CC^[GC]GG 671 CCGGG NspI 255 ACATGC [GA]CATG^[CT] 460 ACATGC RsaI 578 GTAC GT^AC 659 GTAC Sau96I 265 GGTCC G^G[ACGT]CC 555 GGACC SfaNI 760 GATGC GCATC(5/9) 427 GCATC SfcI 467 CTGTAG C^T[GA][CT]AG 847 CTGCAG StyI 854 CCAAGG C^C[AT][AT]GG 869 CCAAGG TfiI 93 GATTC G^A[AT]TC 830 GAATC Tru9I 146 TTAA T^TAA 421 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BbsI BbvI BpuAI BsaJI BssKI BstNI Cac8I DdeI DpnII EcoRII Fnu4HI HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI ScrFI TseI Tsp45I Tsp509I TspRI