################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccII AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsiEI BsiWI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BssHII BstAPI BstEII BstPI BstUI BstXI Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MunI MvnI NarI NciI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 688 GGTACC G^GTACC AccI 642 GTATAC GT^[AC][GT]AC AccIII 254 TCCGGA T^CCGGA AcyI 1931 GGCGTC G[GA]^CG[CT]C AflII 1233 CTTAAG C^TTAAG Asp718I 688 GGTACC G^GTACC AspHI 650 GAGCAC G[AT]GC[AT]^C BalI 1119 TGGCCA TGG^CCA BanI 688 GGTACC G^G[CT][GA]CC BbsI 838 GAAGAC GAAGAC(2/6) BfrI 1233 CTTAAG C^TTAAG BglI 1999 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 838 GAAGAC GAAGAC(2/6) BsaBI 69 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1931 GGCGTC G[GA]^CG[CT]C BseAI 254 TCCGGA T^CCGGA BsiHKAI 650 GAGCAC G[AT]GC[AT]^C BsmBI 1933 CGTCTC CGTCTC(1/5) BspEI 254 TCCGGA T^CCGGA BsrFI 310 GCCGGC [GA]^CCGG[CT] Bst1107I 642 GTATAC GTA^TAC BstBI 1376 TTCGAA TT^CGAA BstZ17I 642 GTATAC GTA^TAC CfoI 1239 GCGC GCG^C Cfr10I 310 GCCGGC [GA]^CCGG[CT] DraII 903 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1135 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1441 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 552 CCTCCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 903 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 1373 GAATTC G^AATTC HgaI 1932 GCGTC GACGC(5/10) HhaI 1239 GCGC GCG^C Hin1I 1931 GGCGTC G[GA]^CG[CT]C HinP1I 1239 GCGC G^CGC HincII 1924 GTTGAC GT[CT]^[GA]AC HindII 1924 GTTGAC GT[CT]^[GA]AC KpnI 688 GGTACC GGTAC^C MamI 69 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1119 TGGCCA TGG^CCA MroI 254 TCCGGA T^CCGGA MscI 1119 TGGCCA TGG^CCA NaeI 310 GCCGGC GCC^GGC NgoMIV 310 GCCGGC G^CCGGC NheI 196 GCTAGC G^CTAGC NspI 1280 GCATGC [GA]CATG^[CT] NspV 1376 TTCGAA TT^CGAA PshAI 1927 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1552 CTGCAG CTGCA^G ScaI 19 AGTACT AGT^ACT SfuI 1376 TTCGAA TT^CGAA SphI 1280 GCATGC GCATG^C SspI 1843 AATATT AAT^ATT XbaI 1497 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 35 CCGC CCGC(-3/-1) 38 CCGC AvaII 46 GGTCC G^G[AT]CC 1910 GGTCC BbvI 28 GCTGC GCAGC(8/12) 1996 GCTGC BseRI 1537 CTCCTC GAGGAG(10/8) 937 GAGGAG BsmI 106 GCATTC GAATGC(1/-1) 544 GCATTC Bsp1407I 281 TGTACA T^GTACA 1160 TGTACA BsrGI 281 TGTACA T^GTACA 1160 TGTACA BssSI 352 CTCGTG CACGAG(-5/-1) 424 CTCGTG EaeI 308 TGGCCG [CT]^GGCC[GA] 1119 TGGCCA MaeII 272 ACGT A^CGT 1601 ACGT MseI 811 TTAA T^TAA 1234 TTAA MspA1I 26 CAGCTG C[AC]G^C[GT]G 149 CAGCTG PflMI 924 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1752 CCAGACCATGG PvuII 26 CAGCTG CAG^CTG 149 CAGCTG SfcI 23 CTACAG C^T[GA][CT]AG 1552 CTGCAG SmlI 293 CTCAAG C^T[CT][GA]AG 1233 CTTAAG SspBI 281 TGTACA T^GTACA 1160 TGTACA TaqI 289 TCGA T^CGA 1377 TCGA TfiI 1150 GATTC G^A[AT]TC 1291 GATTC Tru9I 811 TTAA T^TAA 1234 TTAA TseI 28 GCTGC G^C[AT]GC 1996 GCTGC TthHB8I 289 TCGA T^CGA 1377 TCGA Van91I 924 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1752 CCAGACCATGG XcmI 390 CCATTGTATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1613 CCATTGGGCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI BanII BfaI BmyI BpmI BsaJI BsaWI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tsp45I Tsp509I TspRI XhoII