################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AvaI AvrII BalI BamHI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HapII HincII HindII HindIII HpaI HpaII KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 890 GGTACC G^GTACC AccII 37 CGCG CG^CG AlwI 720 GATCC GGATC(4/5) ApaI 912 GGGCCC GGGCC^C Asp718I 890 GGTACC G^GTACC AspI 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1117 GGCGCC GGCGC^C BbsI 232 GAAGAC GAAGAC(2/6) BbvCI 1045 GCTGAGG CCTCAGC(-5/-2) BglI 10 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 232 GAAGAC GAAGAC(2/6) BsaI 157 GAGACC GGTCTC(1/5) BsgI 844 GTGCAG GTGCAG(16/14) BsmI 579 GAATGC GAATGC(1/-1) BspHI 812 TCATGA T^CATGA BsrBI 1015 GAGCGG CCGCTC(-3/-3) BstUI 37 CGCG CG^CG BstYI 719 AGATCC [GA]^GATC[CT] EarI 423 GAAGAG CTCTTC(1/4) EcoNI 1062 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1117 GGCGCC [GA]GCGC^[CT] KasI 1117 GGCGCC G^GCGCC KpnI 890 GGTACC GGTAC^C Ksp632I 423 GAAGAG CTCTTC(1/4) MflI 719 AGATCC [GA]^GATC[CT] MseI 518 TTAA T^TAA MspA1I 1076 CAGCTG C[AC]G^C[GT]G MvnI 37 CGCG CG^CG NarI 1117 GGCGCC GG^CGCC NspI 773 GCATGC [GA]CATG^[CT] PflFI 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1058 AGGACCT [GA]G^G[AT]CC[CT] PshAI 959 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1076 CAGCTG CAG^CTG RcaI 812 TCATGA T^CATGA SacI 937 GAGCTC GAGCT^C SapI 423 GAAGAGC GCTCTTC(1/4) SbfI 1163 CCTGCAGG CCTGCA^GG SexAI 71 ACCAGGT A^CC[AT]GGT SfoI 1117 GGCGCC GGC^GCC SphI 773 GCATGC GCATG^C Sse8387I 1163 CCTGCAGG CCTGCA^GG TaqI 1145 TCGA T^CGA TfiI 1072 GAATC G^A[AT]TC Tru9I 518 TTAA T^TAA Tth111I 1243 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1145 TCGA T^CGA XcmI 786 CCAAGCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 719 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 46 GGCGTC G[GA]^CG[CT]C 1117 GGCGCC AlwNI 1040 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1231 CAGAGCCTG AocI 330 CCTAAGG CC^T[ACGT]AGG 382 CCTAAGG AviII 176 TGCGCA TGC^GCA 1136 TGCGCA BbvI 709 GCAGC GCAGC(8/12) 801 GCAGC BpmI 1228 CTCCAG CTGGAG(16/14) 55 CTGGAG BsaHI 46 GGCGTC G[GA]^CG[CT]C 1117 GGCGCC BsmAI 109 GAGAC GTCTC(1/5) 157 GAGAC BsmFI 272 GGGAC GGGAC(10/14) 133 GTCCC BstF5I 24 GGATG GGATG(2/0) 1206 GGATG BstXI 20 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1203 CCAGGATGCTGG Bsu36I 330 CCTAAGG CC^T[ACGT]AGG 382 CCTAAGG DpnII 720 GATC ^GATC 1143 GATC DraII 912 GGGCCCT [GA]G^G[ACGT]CC[CT] 1058 AGGACCT Eco81I 330 CCTAAGG CC^T[ACGT]AGG 382 CCTAAGG EcoO109I 912 GGGCCCT [GA]G^G[ACGT]CC[CT] 1058 AGGACCT FauI 35 CCCGC CCCGC(4/6) 632 CCCGC FokI 24 GGATG GGATG(9/13) 1206 GGATG FspI 176 TGCGCA TGC^GCA 1136 TGCGCA HgaI 47 GCGTC GACGC(5/10) 396 GCGTC Hin1I 46 GGCGTC G[GA]^CG[CT]C 1117 GGCGCC HphI 685 GGTGA GGTGA(8/7) 549 TCACC MboI 720 GATC ^GATC 1143 GATC NdeII 720 GATC ^GATC 1143 GATC PstI 746 CTGCAG CTGCA^G 1164 CTGCAG Sau3AI 720 GATC ^GATC 1143 GATC TseI 709 GCAGC G^C[AT]GC 801 GCAGC Tsp45I 548 GTCAC ^GT[GC]AC 1249 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BanI BanII BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DsaI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MslI MvaI MwoI NcoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SfcI SmlI StyI Tsp509I TspRI