(Circular) (Six-base) MAPSORT of: pFN21AE3638.seq Check: 4531 from: 1 to: 5983 pFN21AE3638 5983 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2773 3176 2773 Size: 403 5580 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 2895 3304 2895 Size: 409 5574 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2493 2685 4046 5430 5698 1632 Size: 370 491 192 1361 1384 268 1917 Fragments arranged by size: 1917 1384 1361 491 370 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 3194 1166 Size: 2028 3955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 2497 2529 2138 Size: 32 327 32 5592 Fragments arranged by size: 5592 327 32 32 AlwNI CAG_nnn'CTG Cuts at: 3050 3241 3726 5298 3050 Size: 191 485 1572 3735 Fragments arranged by size: 3735 1572 485 191 ApaI G_GGCC'C Cuts at: 2921 2921 Size: 5983 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 2186 2450 2609 3292 3518 3670 5779 1493 Size: 693 264 159 683 226 152 2109 1697 Fragments arranged by size: 2109 1697 693 683 264 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3308 1870 Size: 73 1365 4545 Fragments arranged by size: 4545 1365 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2647 2895 3098 3122 3304 Size: 325 217 627 860 248 203 24 182 Cuts at: 3304 4759 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 860 627 325 248 217 203 182 24 BanII G_rGCy'C Cuts at: 1391 2012 2040 2067 2921 2946 3234 3302 1391 Size: 621 28 27 854 25 288 68 4072 Fragments arranged by size: 4072 854 621 288 68 28 27 25 BbeI G_GCGC'C Cuts at: 3126 3126 Size: 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2244 928 Size: 971 345 4667 Fragments arranged by size: 4667 971 345 BbvCI CC'TCA_GC Cuts at: 3051 3051 Size: 5983 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 4594 136 Size: 107 122 71 631 605 349 2573 1525 Fragments arranged by size: 2573 1525 631 605 349 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2091 2678 2921 4044 5200 1790 Size: 301 587 243 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 587 301 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2404 3714 Size: 416 53 83 399 24 774 213 1310 Cuts at: 3714 4672 442 Size: 958 1753 Fragments arranged by size: 1753 1310 958 774 416 399 213 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 3218 4643 1239 Size: 842 1137 1425 2579 Fragments arranged by size: 2579 1425 1137 842 Bpu10I CC'TnA_GC Cuts at: 3051 3051 Size: 5983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 2912 2930 2971 4108 4973 5271 2651 Size: 261 18 41 1137 865 298 3363 Fragments arranged by size: 3363 1137 865 298 261 41 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 4646 882 Size: 617 657 2490 2219 Fragments arranged by size: 2490 2219 657 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 3123 3790 4172 Size: 53 83 186 936 519 1071 667 382 Cuts at: 4172 4807 275 Size: 635 1451 Fragments arranged by size: 1451 1071 936 667 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2975 3005 3053 3083 1178 Size: 30 165 30 1572 30 48 30 4078 Fragments arranged by size: 4078 1572 165 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 2720 3284 1186 Size: 1246 288 564 3885 Fragments arranged by size: 3885 1246 564 288 BseYI C'CCAG_C Cuts at: 2011 2517 5186 5682 2011 Size: 506 2669 496 2312 Fragments arranged by size: 2669 2312 506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2870 1163 Size: 573 1134 4276 Fragments arranged by size: 4276 1134 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 2747 2928 2946 4044 4129 5200 2103 Size: 644 181 18 1098 85 1071 2886 Fragments arranged by size: 2886 1098 1071 644 181 85 18 BsmI GAATG_Cn' Cuts at: 2590 3494 3587 2590 Size: 904 93 4986 Fragments arranged by size: 4986 904 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2040 2067 2091 2103 2678 2747 Size: 399 222 28 27 24 12 575 69 Cuts at: 2747 2921 2928 2946 3234 3302 4044 4129 5200 Size: 174 7 18 288 68 742 85 1071 Cuts at: 5200 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 575 399 288 222 174 85 69 68 28 27 24 18 12 7 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 2817 3872 5602 2817 Size: 1055 1730 3198 Fragments arranged by size: 3198 1730 1055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2773 2907 3176 3338 844 Size: 1929 134 269 162 3489 Fragments arranged by size: 3489 1929 269 162 134 BsrBI CCG'CTC Cuts at: 1394 1823 3022 3870 1394 Size: 429 1199 848 3507 Fragments arranged by size: 3507 1199 848 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2032 3215 2032 Size: 1183 4800 BstYI r'GATC_y Cuts at: 1972 2724 3313 3680 4065 4082 5534 5620 5632 Size: 752 589 367 385 17 1452 86 12 Cuts at: 5632 5978 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 752 589 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2388 2336 Size: 52 5931 BtgI C'CryG_G Cuts at: 513 1065 2025 2281 2830 3192 513 Size: 552 960 256 549 362 3304 Fragments arranged by size: 3304 960 552 549 362 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 2806 2958 3499 4293 4313 2806 Size: 152 541 794 20 4476 Fragments arranged by size: 4476 794 541 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948 Size: 54 1425 50 275 18 1600 889 1629 Cuts at: 5948 8 Size: 43 Fragments arranged by size: 1629 1600 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 3913 1353 Size: 1070 1490 3423 Fragments arranged by size: 3423 1490 1070 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2944 2944 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2081 2493 2685 3218 4046 4643 Size: 393 370 79 412 192 533 828 597 Cuts at: 4643 5430 5698 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 597 533 412 393 370 268 192 79 EcoNI CCTnn'n_nnAGG Cuts at: 3071 3071 Size: 5983 EcoO109I rG'GnC_Cy Cuts at: 2918 3064 2918 Size: 146 5837 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2072 2072 Size: 5983 FspI TGC'GCA Cuts at: 2183 3143 4489 2183 Size: 960 1346 3677 Fragments arranged by size: 3677 1346 960 FspAI rTGC'GCAy Cuts at: 3143 3143 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 3126 5130 1678 Size: 1448 2004 2531 Fragments arranged by size: 2531 2004 1448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3053 3085 4624 4656 4698 Size: 32 135 32 1481 32 1539 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 1539 1481 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 3122 3122 Size: 5983 KpnI G_GTAC'C Cuts at: 2899 3308 2899 Size: 409 5574 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2937 5097 5281 1305 Size: 1632 2160 184 2007 Fragments arranged by size: 2160 2007 1632 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2691 2762 2783 2816 3194 3941 Size: 648 864 661 71 21 33 378 747 Cuts at: 3941 4300 4459 518 Size: 359 159 2042 Fragments arranged by size: 2042 864 747 661 648 378 359 159 71 33 21 MspA1I CmG'CkG Cuts at: 1606 3083 3399 4076 5224 5469 1606 Size: 1477 316 677 1148 245 2120 Fragments arranged by size: 2120 1477 1148 677 316 245 NarI GG'CG_CC Cuts at: 3123 3123 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 2025 2281 2830 513 Size: 552 960 256 549 3666 Fragments arranged by size: 3666 960 552 549 256 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2782 3341 4886 1132 Size: 97 1553 559 1545 2229 Fragments arranged by size: 2229 1553 1545 559 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PfoI T'CCnGG_A Cuts at: 3036 3036 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3064 3064 Size: 5983 PshAI GACnn'nnGTC Cuts at: 1613 2968 1613 Size: 1355 4628 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PspOMI G'GGCC_C Cuts at: 2917 2917 Size: 5983 PstI C_TGCA'G Cuts at: 830 2755 3173 3335 830 Size: 1925 418 162 3478 Fragments arranged by size: 3478 1925 418 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 3083 3083 Size: 5983 SacI G_AGCT'C Cuts at: 2946 2946 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2423 2423 Size: 5983 SbfI CC_TGCA'GG Cuts at: 3173 3335 3173 Size: 162 5821 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SexAI A'CCwGG_T Cuts at: 2076 2076 Size: 5983 SfcI C'TryA_G Cuts at: 826 1045 2490 2751 3132 3169 3331 4466 5147 Size: 219 1445 261 381 37 162 1135 681 Cuts at: 5147 5338 826 Size: 191 1471 Fragments arranged by size: 1471 1445 1135 681 381 261 219 191 162 37 SfoI GGC'GCC Cuts at: 3124 3124 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2927 2945 2986 4087 4988 Size: 197 926 723 261 18 41 1101 901 Cuts at: 4988 5250 820 Size: 262 1553 Fragments arranged by size: 1553 1101 926 901 723 262 261 197 41 18 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 2782 3341 1132 Size: 1650 559 3774 Fragments arranged by size: 3774 1650 559 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 2025 2141 2281 2537 2830 3422 513 Size: 552 960 116 140 256 293 592 3074 Fragments arranged by size: 3074 960 592 552 293 256 140 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2386 4010 4027 4180 4365 4810 325 Size: 1255 806 1624 17 153 185 445 1498 Fragments arranged by size: 1624 1498 1255 806 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2481 2604 Size: 274 80 33 51 1443 222 282 123 Cuts at: 2604 4229 5941 96 Size: 1625 1712 138 Fragments arranged by size: 1712 1625 1443 282 274 222 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5943 5975 5943 Size: 32 5951 Tth111I GACn'n_nGTC Cuts at: 1316 3251 1316 Size: 1935 4048 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2798 2798 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI