################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI HincII HindII HindIII HinfI HpaI HpaII KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MslI MspI MunI MvnI MwoI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 32 GGCGCC G[GA]^CG[CT]C AlwI 386 GGATC GGATC(4/5) AlwNI 13 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 171 GAGCAC G[AT]GC[AT]^C BanI 32 GGCGCC G^G[CT][GA]CC BbeI 32 GGCGCC GGCGC^C BbvI 15 GCAGC GCAGC(8/12) BfaI 76 CTAG C^TAG BmyI 171 GAGCAC G[AGT]GC[ACT]^C BsaHI 32 GGCGCC G[GA]^CG[CT]C BsiHKAI 171 GAGCAC G[AT]GC[AT]^C BsiYI 377 CCTGTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 377 CCTGTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 171 GAGCAC G[AGT]GC[ACT]^C BspHI 362 TCATGA T^CATGA BsrDI 109 GCAATG GCAATG(2/0) BstF5I 300 GGATG GGATG(2/0) BstYI 386 GGATCT [GA]^GATC[CT] DpnII 387 GATC ^GATC Fnu4HI 15 GCAGC GC^[ACGT]GC FokI 300 GGATG GGATG(9/13) HaeII 32 GGCGCC [GA]GCGC^[CT] Hin1I 32 GGCGCC G[GA]^CG[CT]C ItaI 15 GCAGC GC^[ACGT]GC KasI 32 GGCGCC G^GCGCC MaeI 76 CTAG C^TAG MaeIII 373 GTCAC ^GT[ACGT]AC MboI 387 GATC ^GATC MflI 386 GGATCT [GA]^GATC[CT] MnlI 153 CCTC CCTC(7/6) NarI 32 GGCGCC GG^CGCC NdeII 387 GATC ^GATC NlaIV 32 GGCGCC GG[ACGT]^[ACGT]CC RcaI 362 TCATGA T^CATGA Sau3AI 387 GATC ^GATC SfaNI 288 GCATC GCATC(5/9) SfoI 32 GGCGCC GGC^GCC TseI 15 GCAGC G^C[AT]GC Tsp45I 373 GTCAC ^GT[GC]AC XhoII 386 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 200 GTAC GT^AC 380 GTAC BalI 2 TGGCCA TGG^CCA 113 TGGCCA BsaJI 203 CCCTGG C^C[ACGT][ACGT]GG 204 CCTGGG BssKI 36 CCTGG ^CC[ACGT]GG 204 CCTGG BstNI 36 CCTGG CC^[AT]GG 204 CCTGG CfoI 33 GCGC GCG^C 107 GCGC DdeI 167 CTGAG C^T[ACGT]AG 248 CTCAG EaeI 2 TGGCCA [CT]^GGCC[GA] 113 TGGCCA EcoRII 36 CCTGG ^CC[AT]GG 204 CCTGG HaeIII 3 GGCC GG^CC 114 GGCC HhaI 33 GCGC GCG^C 107 GCGC HinP1I 33 GCGC G^CGC 107 GCGC HphI 155 TCACC GGTGA(8/7) 374 TCACC MboII 26 GAAGA GAAGA(8/7) 220 GAAGA MluNI 2 TGGCCA TGG^CCA 113 TGGCCA MscI 2 TGGCCA TGG^CCA 113 TGGCCA MspA1I 16 CAGCTG C[AC]G^C[GT]G 230 CAGCTG MvaI 36 CCTGG CC^[AT]GG 204 CCTGG NlaIII 66 CATG CATG^ 363 CATG PvuII 16 CAGCTG CAG^CTG 230 CAGCTG RsaI 200 GTAC GT^AC 380 GTAC ScrFI 36 CCTGG CC^[ACGT]GG 204 CCTGG Tsp509I 329 AATT ^AATT 344 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### AluI